Serological study of hantavirus in man in the Autonomous Community of Madrid, Spain

Lledó, Lourdes; Gegúndez, María Isabel; Saz, José Vicente; Alves, Maria João; Beltrán, María

doi:10.1099/0022-1317-51-10-861

Cited by 13 publications

(18 citation statements)

References 16 publications

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“…In the second study, more than 40% of all seroreactive samples originated from the group of 60-69-year-old individuals (Koch et al, unpublished data). In line with this, the mean age of antihantavirus-positive persons was found to be about 45-60 years in Spain, Austria, and Estonia [10,16,57].…”

Section: Seroprevalence In the Lithuanian Serum Panel Seems To Be Incsupporting

confidence: 50%

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Detection of Human Hantavirus Infections in Lithuania

et al. 2005

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Section: Seroprevalence In the Lithuanian Serum Panel Seems To Be Incsupporting

confidence: 50%

“…In contrast, in Spain, Estonia, and Germany a slight and in Austria a strong dominance of infections in males was reported [10,16,57,Koch et al,unpublished data]. Similarly, in several countries the majority of HFRS patients are males, e.g.…”

Section: Seroprevalence In the Lithuanian Serum Panel Seems To Be Incmentioning

confidence: 69%

Detection of Human Hantavirus Infections in Lithuania

et al. 2005

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“…In Spain, seroprevalence of 0.06% was found in 2003 in more than 10,000 sera from blood donors using methods comparable to those used in our study [22], while other studies published in 2002 and 2009, based on smaller number of samples and using different methods for screening and confirmation, reported a seroprevalence of 0.31% and 2% respectively for distinct areas in Spain [23,24]. In Italy, a serological study on sera collected in 2002 in the north of the country from 488 forestry workers revealed no serum reaction to PUUV and, although a low seroprevalence for hantaviruses (0.4%) was found in bank voles [25]; no human cases of hantavirus infection were reported in Italy between 2000 and 2010 [4,26].…”

Section: Discussionmentioning

confidence: 57%

“…Due to time-consuming preparatory work, the period of blood collection in the military personnel could not be handled flexibly and could not be postponed to the following year: in that year, increased numbers of hantavirus infections were documented in Germany [4,11]. Prevalence data from various populations, including blood donors, risk groups and symptomatic or asymptomatic volunteers from the general population, are available from several European countries, rendering them attractive for comparative analysis between different regions [18,[22][23][24]. But, as we have shown, the methods applied to determine prevalence data need to be considered as well, when comparing different studies, since the influence of the test sensitivity and specificity on the determined seroprevalence may be substantial.…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence of hantavirus infections in Switzerland in 2009: difficulties in determining prevalence in a country with low endemicity

et al. 2013

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In several European countries, diagnosis of nephropathia epidemica, a mild form of haemorrhagic fever with renal syndrome (HFRS) caused by Puumalavirus infection, has increased over the past 10-20 years. In Switzerland, despite its geographical proximity to regions with epidemic outbreaks in Germany and France, infections are detected only sporadically. To estimate the actual prevalence and potential risk factors of human hantavirus infections in Switzerland, a seroepidemiological study was performed in 2009 on serum samples from 4,559 blood donors and 1,810 military personnel. Sera were screened using commercial Puumala IgG and hantavirus IgG enzyme-linked immunosorbent assays indicating a seroprevalence of 1% and 9%, respectively. Subsequently, the samples were analysed by immunofluorescence assay and immunoblot assay, showing a much lower prevalence, of 0.4% and 0.3%, respectively. Two of the serum samples achieved an 80% reduction in plaque-forming units in a neutralisation test. Statistical evaluation of questionnaires only identified an association of age (above 50 years) with hantavirus seropositivity when adjusted for sex (odds ratio: 2.36; 95% confidence interval: 1.10-5.05). This study provides baseline data (0.3-0.4%) for future monitoring of hantavirus seroprevalence in Switzerland and highlights the challenges in estimating the seroprevalence of these viruses in a country with very low endemicity.

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“…In our study the average age of seropositive males was 63.3 years (ages ranging from 35 to 83 years) and for females 52.2 years (ranging from 29 to 71 years). Similarly, seroepidemiological studies in Spain, Austria, Estonia and Lithuania revealed the average age of anti-hantavirus positive subjects to be about 50-65 years [1,9,16,27].…”

Section: Discussionmentioning

confidence: 95%

Seroepidemiological study in a Puumala virus outbreak area in South-East Germany

Mertens

Wölfel

Ullrich³

et al. 2009

Med Microbiol Immunol

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Puumala virus (PUUV) is the cause of the majority of haemorrhagic fever with renal syndrome cases in Germany. In 2004, a nephropathia epidemica outbreak was recorded in Lower Bavaria, South-East Germany. For a seroepidemiological study in this region including the resident population at four locations (n = 178) and soldiers from one location (n = 208) indirect immunoglobulin M (IgM) and immunoglobulin G (IgG) enzyme-linked immunosorbent assays (ELISAs) and immunoblot tests based on a yeast-expressed PUUV nucleocapsid protein were established. The validation using human serum panels originating from Germany revealed a diagnostic sensitivity and specificity of 98/100% for the IgM ELISA, 99/99% for the IgG ELISA, 99/100% for the IgM immunoblot test and 100/96% for the IgG immunoblot test. Using the novel IgG assays as well as a commercial IgG ELISA and an immunofluorescence assay for the resident population an average prevalence of 6.7% (12 of 178) with a range of 0% (0 of 21) to 11.9% (7 of 59) was observed. Positive serological results were equally distributed between males and females with an average age of 63 for males and 52 for females. The seroprevalence in the soldier group was found to be about 1% with one positive male of 203 (age 46 years) and one positive female of five (age 47 years). In conclusion, the PUUV seroprevalence in the residents of the outbreak region in Lower Bavaria was found to be up to fivefold higher than the average hantavirus seroprevalence of the German population.

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Serological study of hantavirus in man in the Autonomous Community of Madrid, Spain

Cited by 13 publications

References 16 publications

Detection of Human Hantavirus Infections in Lithuania

Detection of Human Hantavirus Infections in Lithuania

Seroprevalence of hantavirus infections in Switzerland in 2009: difficulties in determining prevalence in a country with low endemicity

Seroepidemiological study in a Puumala virus outbreak area in South-East Germany

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