Serological Studies of Infectious Bronchitis Vaccines against Japanese Field Isolates of Homologous and Heterologous Genotypes

Shimazaki, Yohko; Harada, Makiko; Horiuchi, Takashi; Yoshida, Keiko; Tanimura, Chieko; Nakamura, Shigeyuki; Mase, Masaji; Suzuki, Shinichi

doi:10.1292/jvms.71.891

Cited by 10 publications

(10 citation statements)

References 19 publications

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“…Sequence analysis of Sulaimani isolate S1 gene revealed its close relatedness only to three field isolates in Israel and Egypt (Table 2). Since high rate of antiserum immunized with different homologous field genotype could neutralize each other (Shimazaki et al, 2009;Wang and Huang, 2000), which suggest genotypes based on S1 sequence analysis to some extent correlate with serotypes of IBV strains, therefore Isolates from Egypt, Iraq and Israel can be considered as the same novel Middle East genotype, and most likely to be the same serotype.…”

Section: Discussionmentioning

confidence: 99%

Isolation and molecular characterization of Sul/01/09 avian infectious bronchitis virus, indicates the emergence of a new genotype in the Middle East

Mahmood¹,

Sleman

Uthman

2011

Veterinary Microbiology

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Infectious bronchitis virus (IBV) was isolated from trachea and kidney tissues of eight broiler farms in Kurdistan region of North Iraq from 2008 to 2010. The birds were suffering from respiratory and nephropathological symptoms and lesions. A 1116 bp hyper mutable spike glycoprotein (S1) gene was amplified and sequenced using conventional RT-PCR. Sequence analysis and BLAST homology search in GenBank data base indicate that two of the farms were infected with the 4/91 strain, one with an unidentified IBV and five were infected with Sul/01/09. The birds in the latter five farms were suffering from nephropathogenic lesions, however, the virus was isolated from kidney but not from trachea in these cases. The birds were vaccinated regularly with 4/91 or MA5 vaccine. The deduced amino acid sequence of the isolated and amplified S1 subunit (372 aa) of Sul/01/09 was differed in 27-28% from that of all three vaccine strains (4/91, MA5, and H120) used in the region. This dissimilarity is most likely the cause of poor efficacy of vaccines used in the region, at least in five of these farms. Amino acid sequence comparison and phylogenetic tree analysis with other published IBV genotypes indicate that this newly isolated virus together with other regionally related and recently published isolates from Israel (IS/720/99, IS/885) and Egypt (egypt/Benisuef/01) belong to a new genotype. This is the first report of identification and genotyping of IBV isolate in Iraq, which indicate the circulation of 4/91 along with a new variant (Sul/01/09) of IBV in vaccinated broiler farms.

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Section: Discussionmentioning

confidence: 99%

Isolation and molecular characterization of Sul/01/09 avian infectious bronchitis virus, indicates the emergence of a new genotype in the Middle East

Mahmood¹,

Sleman

Uthman

2011

Veterinary Microbiology

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“…It is noteworthy that the detection of 4/91 strains in Japan began after marketing of the 4/91 vaccine in this country. Shimazaki [14] reported that one of the 4/91 isolates in Japan might be derived from the vaccine, and others have been closely linked to French and Spanish isolates. In the U.S.A., after the introduction of a new vaccine from the Netherlands, a new IBV variant became endemic, and the S1 gene sequence was highly homologous to that of the vaccine strain [13].…”

Section: Discussionmentioning

confidence: 99%

Classification of IBV S1 Genotypes by Direct Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) and Relationship between Serotypes and Genotypes of Strains Isolated between 1998 and 2008 in Japan

Ariyoshi

Kawai

Honda

et al. 2010

The Journal of Veterinary Medical Science

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ABSTRACT. In this study, we attempted to establish a simple detection method for classification of IBV S1 genotypes by direct reverse transcriptase-polymerase chain reaction (RT-PCR). Then, to evaluate the usefulness of the S1 genotype-specific RT-PCR, we examined the relationship between S1 genotypes and serotypes of IBV in Japan. Sequencing of the S1 genes of IBV and phylogenetic tree analysis were conducted. On the basis of the sequencing data of the S1 genotype samples, we determined primer sets specific for each genotype. Five vaccine strains in Japan as reference strains and 46 field isolates were classified into different genetic clusters by phylogenetic tree analysis (JP-1, JP-II, JP-III, Mass and 4/91) and were matched to the results of S1 genotype-specific RT-PCR. A cross virus-neutralizing test showed that the five vaccine strains in Japan exhibited different serotypes from each other. The concordance rate of the 46 field isolates between the S1 genotypes and serotypes was 65.2%. The present study indicates that genotype-specific RT-PCR could be a convenient and useful tool for determining IBV serotypes and could contribute to the control of IBV outbreaks in Japan.

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“…This vaccine could be predicted in accordance with the genotype of field isolates. And since the S1 glycoprotein is responsible for infection of host cells, consequently the S1 protein is usually used for IBV typing [67] . Combination of antigenic epitopes of B cells and T cells that are conserved across many strains as a approach to evoke humoral and cytotoxic T cell (CTI) immune responses will potentially lead to a broad -based vaccine that could reduce the challenges in using live attenuated vaccine technology in the control of IBV infection in poultry .…”

Section: Designing Of Vaccine Epitopesmentioning

confidence: 99%

Untitled

2018

RJLBPCS

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Infectious bronchitis disease is a contiguous disease in poultry, treated mainly by vaccination which is failed at high rate due to high variation of the Infectious Bronchitis Virus (IBV).Epitope -based vaccine as promising approach was used. In this study epitopes were derived from consensus sequence of vaccine strains used in Iraq, characterized and some of them docked with chicken MHC I molecules. Two B cell epitopes were obtained:51-59 (THTAQSGYY), 76-84(FMYGSYHPS) which satisfied the required criteria. Five CLT epitopes: 22-30 (TLELTNFTF), 57-65(GYYNFNFSF), 68-76(SFVYKESNF), 73-81(ESNFMYGSY), 99-107(NSLSVSLAY) were obtained according to acceptable criteria and docked very well with MHC I molecules of chickens .Six epitopes for helper T cell were obtained: 56-70(SGYYNFNFSFLSSFV), 57-71(GYYNFN FSFLSSFVY), 58-72(YYNFNFSFLSSFVYK), 61-75(FNFSFLSSFVYKESN) ,63-77(FSFLSSF VYKESNFM) ,64-78(SFLSSFVYKESNFMY), they react with many HLA alleles at high affinity.

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Serological Studies of Infectious Bronchitis Vaccines against Japanese Field Isolates of Homologous and Heterologous Genotypes

Cited by 10 publications

References 19 publications

Isolation and molecular characterization of Sul/01/09 avian infectious bronchitis virus, indicates the emergence of a new genotype in the Middle East

Isolation and molecular characterization of Sul/01/09 avian infectious bronchitis virus, indicates the emergence of a new genotype in the Middle East

Classification of IBV S1 Genotypes by Direct Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) and Relationship between Serotypes and Genotypes of Strains Isolated between 1998 and 2008 in Japan

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