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Abstract

Infectious bronchitis disease is a contiguous disease in poultry, treated mainly by vaccination which is failed at high rate due to high variation of the Infectious Bronchitis Virus (IBV).Epitope -based vaccine as promising approach was used. In this study epitopes were derived from consensus sequence of vaccine strains used in Iraq, characterized and some of them docked with chicken MHC I molecules. Two B cell epitopes were obtained:51-59 (THTAQSGYY), 76-84(FMYGSYHPS) which satisfied the required criteria. Five CLT epitopes: 22-30 (TLELTNFTF), 57-65(GYYNFNFSF), 68-76(SFVYKESNF), 73-81(ESNFMYGSY), 99-107(NSLSVSLAY) were obtained according to acceptable criteria and docked very well with MHC I molecules of chickens .Six epitopes for helper T cell were obtained: 56-70(SGYYNFNFSFLSSFV), 57-71(GYYNFN FSFLSSFVY), 58-72(YYNFNFSFLSSFVYK), 61-75(FNFSFLSSFVYKESN) ,63-77(FSFLSSF VYKESNFM) ,64-78(SFLSSFVYKESNFMY), they react with many HLA alleles at high affinity.