Serological screening for the prevention of transfusion-acquired cytomegalovirus infection

Direct assessment of cytomegalovirus transfusion‐transmitted risks after universal leukoreduction

“…For the 3442 samples with a test result, 35% were seropositive for CMV total antibodies. The positive rate is similar to the previously reported 30%‐70% range 13,36,37 …”

“…The positive rate is similar to the previously reported 30%-70% range. 13,36,37 For the 46 CMV recipients who were seronegative at baseline, there were 1110 available retained linked donor samples and an additional 134 samples that were operationally tested at the donor center (total 1244); 460 (36.9%, 460/1244) were positive for CMV total antibody. Overall 41% of donors were female and 59% were male.…”

Detection of antibody to cytomegalovirus-induced early antigens and comparison with four serologic assays and presence of viruria in blood donors

“…The prevalence of CMV seropositivity in our donor group was relatively low compared with that in other regions of the United States and other parts of the world (1, 10). However, the prevalence of EA-ab (16%) in our donor group was similar to that (10%) in a study of 500 Italian blood donors, in which the seropositivity prevalence was 76% (14). In both groups, the EA-ab-positive donors represented a subset of the seropositive donors, in which proportionately more females than males were EA-ab positive.…”

Impairment of cytomegalovirus and host balance in elderly subjects.

“…A serological sign of active or recent infection was the concomitant presence of a titre of IgG anti-CMV induced LA of > 1/320, anti-EA of > 1/20, and anti-IEA of > 1/20; or the presence of IgM against CMV-induced LA of > 1/20. 5 To prepare IEA induced by CMV, human embryo fibroblasts infected with the Towne strain ofCMV and grown on coverslips were fixed in acetone one hour after infection. EA induced by CMV were prepared by fixing cells infected in the presence of 75 ,ug/ml of cytarabine, 72 hours after infection, to accumulate all EA synthesised before replication of viral DNA.…”