Serological responses of rams to a Brucella ovis—vitamin E adjuvant vaccine

Tengerdy, Robert P.; Ameghino, Enrique; Riemann, Hans

doi:10.1016/0264-410x(91)90111-i

Cited by 11 publications

(4 citation statements)

References 9 publications

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“…Contrary to above Franchini et al (1991) and Muneer et al (1994) did not observe any significant beneficial effect of vitamin E on humoral immune response against E. coli in poultry and Pasteurella multocida type 6B immunization in buffalo calves. However, significant immunoenhancing effect of vitamin E has been reported against Brucella ovis (Afzal et al, 1984; Tengerdy et al, 1991), Clostridium perfringens type D vaccine in sheep (Tengerdy et al, 1983) and Mycoplasma mycoides subsp. capri in rabbit (Rana and Srivastava, 1999) and P. multocide whole cell vaccine (Rawat and Jaiswal, 2004) when administered as a locally concentrated preparation in the microenvironment of water-in-oil depot of injected vaccine.…”

Section: Humoral Immune Responsementioning

confidence: 99%

Effect of Dietary Vitamin E Supplementation on Serum 慣-Tocopherol and Immune Status of Crossbred Calves

Samanta¹,

Dass²,

Rawat³

et al. 2006

Asian Australas. J. Anim. Sci

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An experiment was conducted with twenty crossbred male calves (7-15 days old) divided into 4 different experimental groups on the basis of body weights to study the effect of vitamin E supplementation on the serum α-tocopherol concentration and immune response of the calves. All the calves were fed on milk and calf starter up to 13 weeks and afterwards, they were fed on concentrate mixture and oat hay up to 32 weeks of age. In addition, the calves in groups I, II, III and IV were supplemented with 0, 125, 250 and 500 IU feed grade DL-α-tocopheryl acetate, respectively. Blood samples were collected at 0 day and subsequently at 1, 2, 3, 4, 6 and 8 months of age to monitor the serum α-tocopherol concentration in crossbred calves. After 24 weeks of experimental feeding, 4 animals from each group were intramuscularly inoculated with single dose (3 ml) of Haemorrhagic septiceaemia (Pasteurella multocida P52 strain) oil adjuvant vaccine. The cumulative group mean serum α-tocopherol concentration (µg/100 ml) was 88.12, 210.11, 235.21 and 294.02 in-groups I, II, III and IV, respectively and differed significantly (p<0.001) among the four groups. Lymphocyte stimulation indices (LSI) did not differ among the groups significantly. The pooled mean ELISA antibody titer against Pasteurella multocida (P52 strain) was 788.02, 926.85, 1,214.00 and 1,109.51 for group I, II, III and IV, respectively, which indicated higher antibody titer in groups supplemented with vitamin E as compared to the control group. It may be concluded that vitamin E supplementation increased the αtocopherol concentration in serum and dietary supplementation of vitamin E at higher level has a humoral immune enhancing effect against killed bacterial antigen.

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Section: Humoral Immune Responsementioning

confidence: 99%

Effect of Dietary Vitamin E Supplementation on Serum 慣-Tocopherol and Immune Status of Crossbred Calves

Samanta¹,

Dass²,

Rawat³

et al. 2006

Asian Australas. J. Anim. Sci

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“…The effect of vitamin E on the functional and proliferative activity of immune cells has been exploited to evaluate its influence when it is added to emulsified vaccines for chicks (Franchini et al, 1986(Franchini et al, , 1988(Franchini et al, , 1991Tengerdy et al, 1990) and mammals (Afzal et al, 1984;Tengerdy et al, 1991). Previous research (Franchini et al, 1986(Franchini et al, , 1988(Franchini et al, , 1991 using different quantities of vitamin E and mineral oils demonstrated the enhancing effect of this vitamin on the immune response to viral and bacterial antigens.…”

Section: Introductionmentioning

confidence: 95%

Vitamin E in Viral Inactivated Vaccines

et al. 1995

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This research aimed at verifying whether vitamin E added to inactivated and emulsified vaccines enhances the immune response to viral antigens in chicken. Three hundred and twenty broilers (males and females) and 16 types of vaccines, varying in viral antigen [Newcastle disease virus, egg drop syndrome 1976 virus (EDS76V), and infectious bursal disease virus] and vitamin E amount (replacing 10, 20, and 30% of mineral oil) were used. Results show that vaccines with vitamin E, especially when it replaces 20 or 30% of mineral oil, induces a more rapid and higher antibody response than control vaccines. An adjuvant effect of vitamin E was also present in viral vaccine lacking bacterial antigens. Apart from vitamin E content, the Newcastle disease virus and infectious bursal disease virus monovalent vaccines induced higher titers of specific circulating antibodies in birds than did trivalent vaccines.

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“…Kohno et al 27) and Psomiadou and Tsimidou, 28) on the basis of their photo-oxidation studies even claimed that squalene regenerates α-tocopherol and not conversely. In our former experiments, 15) there was observed a low antioxidant activity of α-tocopherol only if it was added to the system after irradiation.…”

Section: Discussionmentioning

confidence: 99%

Antioxidative Protection of Squalene Adjuvant and Rabies Vaccine with Adjuvant

Ondrejková

Süli

Harvanová

et al. 2017

Biological & Pharmaceutical Bulletin

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The authors verified the possibility of antioxidative protection of squalene adjuvant emulsions by the antioxidants α-tocopherol and β-carotene. They determined the influence of β-carotene on the stability and antigenic effectiveness of adjuvant emulsion in combination with rabies vaccine. The composition of the adjuvant emulsions or vaccines was: 2.5% squalene; 6% detergents; 0.5% antioxidant; 91% water phase. The oxidative injury after UV-irradiation was followed by the detection of the peroxide value of the emulsions. The stability of the emulsions was evaluated by the determination of the emulsion's particle size. The level of rabies antibodies (RAB) in mice sera until day 90 after vaccination, was determined by the rapid fluorescent focus inhibition test. In the in vitro system of squalene adjuvant, α-tocopherol acted as a prooxidant, while β-carotene effectively reduced the oxidative injury. The homogenization of the squalene adjuvant during a prolonged period from 8 to 10 min did not change the particle size. The oxidation processes were efficiently reduced by β-carotene during the preparation process and also during the 70-d storage. The vaccine with β-carotene induced a gradual increase in the RAB levels with the highest value on day 28. While the inactivated rabies vaccine with adjuvant without β-carotene developed a rapid formation of RAB, the application of the vaccine with β-carotene induced a slower but more uniform production of RAB. The level of RAB was significantly higher after the application of the vaccine with β-carotene and reached the protective value of 0.5 IU/mL, in contrast to the vaccine without β-carotene.

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Serological responses of rams to a Brucella ovis—vitamin E adjuvant vaccine

Cited by 11 publications

References 9 publications

Effect of Dietary Vitamin E Supplementation on Serum 慣-Tocopherol and Immune Status of Crossbred Calves

Effect of Dietary Vitamin E Supplementation on Serum 慣-Tocopherol and Immune Status of Crossbred Calves

Vitamin E in Viral Inactivated Vaccines

Antioxidative Protection of Squalene Adjuvant and Rabies Vaccine with Adjuvant

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