Serological responses of cattle after treatment and during natural re-infection with Fasciola hepatica, as measured with a dot-ELISA system

Castro, Elisabeth; Freyre, A.; Hernández, Zully

doi:10.1016/s0304-4017(00)00228-4

Cited by 26 publications

(11 citation statements)

References 19 publications

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“…Based on our results, the longevity of the parasites could be longer than 26 months, as the multiparous cows from farm C2 that had no access to outdoor areas (except for dry cows in a sandy yard) or freshly-cut grass, were still seropositive at 4 years of age and over. Lasting antibodies after elimination of the parasites is a possible scenario, but antibody levels declined within 195 days post-treatment in the present study and similar findings have been seen in other studies [51, 52]. Additionally, copro-antigen ELISA values were above the cut-off and liver fluke eggs were present in the faeces in some of these older animals, indicating active infection.…”

Section: Discussionsupporting

confidence: 89%

Patterns of Fasciola hepatica infection in Danish dairy cattle: implications for on-farm control of the parasite based on different diagnostic methods

et al. 2018

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BackgroundBovine fasciolosis is an economically important livestock disease in Europe, and represents a particular challenge for organic farms, where cattle are grazed extensively and the use of anthelmintic is limited. A two-year longitudinal study was conducted on two conventional and two organic Danish dairy farms to examine the current temporal trend of F. hepatica infection on-farm, and to gather data of practical relevance for parasite control. Data were collected both at the herd and individual level using currently available diagnostic methods: a commercial serum antibody ELISA, a commercial copro-antigen ELISA, faecal egg counts, and monthly bulk tank milk (BTM) ELISA. The temporal patterns (animal age, farm-level temporal trends and seasonality) in the animal-level test results were analysed by generalised additive mixed models (GAMM).ResultsPatterns of infection differed substantially between the farms, due to different grazing management and anthelmintic use. However, animals were first infected at the age of 1.5–2 years (heifers), and most at-risk animals sero-converted in autumn, suggesting that summer infections in snails prevail in Denmark. Our results also suggest that the lifespan of the parasite could be over 2 years, as several cows showed signs of low grade infection even after several years of continuous indoor housing without access to freshly-cut grass. The serum antibody ELISA was able to detect infection first, whereas both copro-antigen ELISA and faecal egg counts tended to increase in the same animals at a later point. Decreasing BTM antibody levels were seen on the two farms that started anthelmintic treatment during the study.ConclusionsWhile important differences between farms and over time were seen due to varying grazing management, anthelmintic treatment and climatic conditions, the young stock was consistently seen as the main high-risk group and at least one farm also had suspected transmission (re-infection) within the lactating herd. Careful interpretation of test results is necessary for older cows as they can show persistent infections several years after exposure has stopped. Rigorous treatment regimens can reduce BTM ELISA values, but further research is needed to develop a non-medicinal approach for sustainable management of bovine fasciolosis.Electronic supplementary materialThe online version of this article (10.1186/s13071-018-3248-z) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 89%

Patterns of Fasciola hepatica infection in Danish dairy cattle: implications for on-farm control of the parasite based on different diagnostic methods

et al. 2018

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“…The different methods initially developed employed somatic extracts, and then used the excretory-secretory products, partially purified or recombinant antigens (Pfister et al 1984;Hillyer and Soler de Galanes 1991;Cornelissen et al 1992;El-Bahi et al 1992;Rickard, 1995;Gorman et al 1995;Martínez et al 1996;Anderson et al 1999;Castro et al 2000;Carnevale et al 2001). The advantage of these assays is that it is possible to detect 2-3 weeks post-infection, reaching a peak at 8-10 weeks, being applicable during acute and latent phases of the disease (Santiago and Hillyer 1988;Reichel 2002).…”

Section: Discussionmentioning

confidence: 97%

Molecular diagnosis of natural fasciolosis by DNA detection in sheep faeces

Carnevale

Pantano

Kamenetzky

et al. 2015

Acta Parasitologica

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Fasciolosis is an important parasitic zoonosis considered the most important helminth infection of ruminants in tropical countries. The aim of this study was to develop a PCR assay for the sensitive and specific detection of F. hepatica in formalin preserved sheep faeces. A 405-bp fragment of the cytochrome c oxidase subunit 1 gene of F. hepatica was amplified from stool samples of infected sheep. The PCR assay showed a detection limit of 20 pg of F. hepatica DNA. No cross-reactions were observed with samples containing coccidian oocysts or gastrointestinal nematodes eggs. Our PCR technique showed to be effective for specific detection of F. hepatica infections in sheep.

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“…Several groups reported the use of dot-ELISA for screening anti-Fasciola antibodies in cattle (Ibarra et al 1998;Maisonnave 1999;Castro et al 2000), in llamas (Rickard 1995), in sheep (Zimmerman et al 1985;Arriaga de Morilla et al 1989), and in rabbits (Rivera Marrero et al 1988). This serodiagnostic test has also been applied to diagnose human fasciolosis with an emphasis on crude helminthic antigens (Itoh and Sato 1990;Shaheen et al 1994) or partially purified antigen of somatic adult worms (Shaheen et al 1989).…”

Section: Discussionmentioning

confidence: 98%

Development and evaluation of a dipstick assay in diagnosis of human fasciolosis

Ali

2011

Parasitol Res

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For the diagnosis of human fasciolosis, a new dipstick assay was developed and its diagnostic efficacy was evaluated in comparison to enzyme linked immunosorbent assay (ELISA) using Fasciola gigantica excretory-secretory antigen. Sera samples were collected from patients with confirmed fasciolosis and patients with other parasitic diseases as well as from normal healthy individuals. The sensitivity, specificity, and diagnostic accuracy of the dipstick assay as well as ELISA were 100%, 96.7%, and 98.3%, respectively. The dipstick assay is extremely easy to apply, rapid (15 min), with a visually interpreted result, field portable, inexpensive, reagent conservative, and as being both sensitive and specific in diagnosis of human fasciolosis; the test could be an acceptable alternative for use in clinical laboratories lacking specialized equipment needed for ELISA.

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Serological responses of cattle after treatment and during natural re-infection with Fasciola hepatica, as measured with a dot-ELISA system

Cited by 26 publications

References 19 publications

Patterns of Fasciola hepatica infection in Danish dairy cattle: implications for on-farm control of the parasite based on different diagnostic methods

Patterns of Fasciola hepatica infection in Danish dairy cattle: implications for on-farm control of the parasite based on different diagnostic methods

Molecular diagnosis of natural fasciolosis by DNA detection in sheep faeces

Development and evaluation of a dipstick assay in diagnosis of human fasciolosis

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