Development and evaluation of a dipstick assay in diagnosis of human fasciolosis

Ali, Nematallah

doi:10.1007/s00436-011-2678-8

Cited by 14 publications

(6 citation statements)

References 31 publications

(35 reference statements)

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“…Such a rapid test could therefore be practically implemented in epidemiological surveys. A sensitivity of 100% and a specificity of 96·7% have been preliminarily noted (Ali, 2012), although appropriate independent complete evaluation is still pending.…”

Section: Diagnosis With Blood Samplesmentioning

confidence: 99%

Diagnosis of human fascioliasis by stool and blood techniques: update for the present global scenario

2014

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Before the 1990s, human fascioliasis diagnosis focused on individual patients in hospitals or health centres. Case reports were mainly from developed countries and usually concerned isolated human infection in animal endemic areas. From the mid-1990s onwards, due to the progressive description of human endemic areas and human infection reports in developing countries, but also new knowledge on clinical manifestations and pathology, new situations, hitherto neglected, entered in the global scenario. Human fascioliasis has proved to be pronouncedly more heterogeneous than previously thought, including different transmission patterns and epidemiological situations. Stool and blood techniques, the main tools for diagnosis in humans, have been improved for both patient and survey diagnosis. Present availabilities for human diagnosis are reviewed focusing on advantages and weaknesses, sample management, egg differentiation, qualitative and quantitative diagnosis, antibody and antigen detection, post-treatment monitoring and post-control surveillance. Main conclusions refer to the pronounced difficulties of diagnosing fascioliasis in humans given the different infection phases and parasite migration capacities, clinical heterogeneity, immunological complexity, different epidemiological situations and transmission patterns, the lack of a diagnostic technique covering all needs and situations, and the advisability for a combined use of different techniques, at least including a stool technique and a blood technique.

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Section: Diagnosis With Blood Samplesmentioning

confidence: 99%

Diagnosis of human fascioliasis by stool and blood techniques: update for the present global scenario

2014

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“…Fasciolosis can affect human beings and all ruminant animals of agricultural importance [4], [5]. The geographically wide distribution of these parasites makes fasciolosis one of the most important helminth diseases with a global distribution [6]–[9].…”

Section: Introductionmentioning

confidence: 99%

Comparative Characterization of MicroRNAs from the Liver Flukes Fasciola gigantica and F. hepatica

et al. 2012

PLoS ONE

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MicroRNAs (miRNAs) are key regulators of gene expression at the post-transcription level. The present study specifically explored and compared the miRNA expression profiles of F. gigantica and F. hepatica using an integrated sequencing and bioinformatics platform and quantitative real-time PCR. Nineteen and 16 miRNA candidates were identified from F. gigantica and F. hepatica, respectively. The two parasites shared 11 miRNAs, with 8 also showing similarity to miRNAs of Schistosoma japonicum. Another 8 miRNAs were identified as F. gigantica-specific and 5 as F. hepatica-specific, most of which were novel. Predicted target analysis with 11465 mRNA and EST sequences of F. hepatica and F. gigantica revealed that all of the miRNAs had more than one target, ranging from 2 to 398 with an average of 51 targets. Some functions of the predicted targets were only found in F. gigantica, such as “transcription regulator”, while some others were only found in F. hepatica, such as “reproduction” and “response to stimulus”, indicating the different metabolism and gene regulation patterns of the two parasites. The present study represents the first global comparative characterization of miRNA expression profiles of F. gigantica and F. hepatica, which has provided novel valuable resources for a better understanding of the two zoonotic trematodes.

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“…Therefore, it requires more diagnostic tools to assist in the diagnosis. In the acute stage, serum immunological examination, such as ELISA, indirect fluorescent antibody test (IFA), indirect hemagglutination test (IHA), and counter-current immuneelectrophoresis (CIE), can be used as identification tools (16)(17)(18)(19). But the serological tests may have similar results with other schistosome infections.…”

Section: Discussionmentioning

confidence: 99%

Case Report: Fascioliasis Hepatica Precisely Diagnosed by Metagenomic Next-Generation Sequencing and Treated With Albendazole

Zhang

Liu

et al. 2021

Front. Med.

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The clinical manifestations of fascioliasis hepatica in humans are unspecific. Traditional diagnosis relies on evidence of live parasites or eggs in the bile or feces. However, due to similar imaging manifestations, they are often misdiagnosed as malignant tumors. Here, we report a case of a 43-year-old woman with fever and space-occupying liver disease. Liver biopsy, parasite-specific antibody screening, and stool testing did not find any pathogens. Therefore, metagenomic next-generation sequencing (mNGS) and routine microbiological examinations were performed. Finally, Fasciola hepatica was only identified by mNGS. The body temperature of the patient and the eosinophil count remained normal, and the space-occupying liver lesions were significantly absorbed after more than 7 months of treatment with albendazole. The details of this case highlight the timely use of mNGS to identify parasites and judge therapeutic effects after treatment, providing important help for clinical decision-making.

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Development and evaluation of a dipstick assay in diagnosis of human fasciolosis

Cited by 14 publications

References 31 publications

Diagnosis of human fascioliasis by stool and blood techniques: update for the present global scenario

Diagnosis of human fascioliasis by stool and blood techniques: update for the present global scenario

Comparative Characterization of MicroRNAs from the Liver Flukes Fasciola gigantica and F. hepatica

Case Report: Fascioliasis Hepatica Precisely Diagnosed by Metagenomic Next-Generation Sequencing and Treated With Albendazole

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