SEROLOGICAL RELATIONSHIP BETWEEN
            <i>CLOSTRIDIUM BIFERMENTANS</i>
            AND
            <i>CLOSTRIDIUM SORDELLII</i>
            BASED UPON SOLUBLE ANTIGENS

Ellner, Paul D.; Green, Stanley S.

doi:10.1128/jb.86.3.605-605.1963

Cited by 7 publications

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“…previously reported that Clostridium bifermentans could be obtained by exposing a pathogenic strain of C. sordellii to heat. That C. bifermentans and C. sordellii share some common antigens has been demonstrated by many workers (Clark and Hall, 1937;Stewart, 1938;Tardieux and Nissman, 1952; Tataki and Huet, 1953;Huang, 1959;Walker, 1963;Ellner and Green, 1963). For instance, on investigating the relationship of urease activity and agglutinability of this group of organisms, Tataki and Huet (1953) reported that an anti-C. sordellii serum prepared with a toxigenic strain (C. sordellii 82) could agglutinate to high titers all the urease-positive strains, including those lacking pathogenicity.…”

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confidence: 88%

“…In both cases, the changes were associated not only with simultaneous loss of urease activity and toxigenicity, but also with acquisition of the antigenicity of C. bifermentans. Walker (1963) found no distinction between C. bifermentans and C. sordellii by precipitation ring test, but Ellner and Green (1963) found that each of these two species possessed unique species-specific antigen, as revealed by agar-gel diffusion test when absorbed serum was used. In the light of previous findings in this laboratory, we applied serological methods to test whether the heat-resistant substrains of pathogenic C. sordellii have also changed in their antigenicity from that of C. sordellii toward that of C. bifermentans.…”

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confidence: 99%

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Taxonomy of Clostridium bifermentans and Clostridium sordellii III. Agglutinability of Heat-Resistant Substrains of Clostridium sordellii

1965

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By cross-agglutination tests with Clostridium bifermentans and C. sordellii antisera, the heat-resistant substrains from each pathogenic strain of C. sordellii were found to possess antigens of C. bifermentans in addition to displaying changes in biological activities. The agglutinability and biological activities varied with the sporulating potency acquired. Agglutinin-absorption tests further proved that heat-resistant substrains of C. sordellii were immunologically identical to C. bifermentans.

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confidence: 88%

mentioning

confidence: 99%

Taxonomy of Clostridium bifermentans and Clostridium sordellii III. Agglutinability of Heat-Resistant Substrains of Clostridium sordellii

1965

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“…Beide Spezies lassen sich ohneAbsorption von allen anderen untersuchten Stammen abgrenzen.Abbildung 4 gibt die graphische Auswertung der Ergebnisse der Versuche mit Cl. biferrnentuns(7) wieder. Die Ergebnisse der Absorption sind Tabelle 5 zu entnehmen.…”

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Untersuchungen über die Möglichkeit zur serologischen Differenzierung von Clostridium species mittels Enzym‐Immun‐Verfahren

Reiche

Böhm

1991

Journal of Veterinary Medicine, Series B

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Investigation on Serotyping and Diagnosis of Clostridiu by Enzyme-Immuno-AssayA method for serotyping and diagnosis of 30 selected Clostridia strains of 12 different species hy an enzyme-immuno-assay using polystyrene balls as a solid phase is described and tested for its efficiency. The reference strains and the homologous test strains could be differentiated from the heterologous in principle. A reliable detection of the several serotypes of CI. pefringens was not perfectly to achieve with the conjugates used here, cross-reaction with CI. chuuvoei could be removed by absorption. The conjugate of CI. bifermentuns reacted with CI. sordellii and CI. difficile too, and must be absorbed prior any diagnostic application with vegetative cells of the corresponding strains.Concerning the antigenic relationships between the Clostridia strains used here, the results of ELLNER and GREEN (7, 8) as well as of POXTON and BYRNE (14) could be reconfirmed. Moreover the applied technique offers the possibility to come to a more detailed result concerning the degree of antigenic relationship. The developed method is a valuable tool for the differentiation of pathogenic Clostridia and within the group Cl. bifermentanslC1. sordellii/Cl. difficile a rapid diagnosis at the species level could be achieved. Some limitations by cross-reactions are given, but results can be improved by absorption of the conjugates. REICHE und BOHMDie Diagnostik der Clostridien basiert derzeit auf der Erfassung morphologischer Parameter, von Stoffwechseleigenschaften und auf den Ergebnissen des Tierversuchs (Toxinbildungsvermogen) (16). Die Anwendung gaschromatographischer oder serologischer Techniken setzt sich nur zogernd durch (1 7). Gerade dem Enzym-Immun-Verfahren kommt wegen des geringen Zeitbedarfs und der relativ einfachen Durchfiihrung mit bereits vorhandenen Laborgeraten immer mehr Bedeutung in der serologischen Diagnostik zu.Basierend auf den Erkenntnissen von BOHM et al. (2, 3, 4, 5 ) wurde ein Festphasen-Enzym-Immun-Verfahren entwickelt und erprobt. Im Rahmen der Untersuchungen von NIEDERW~HRMEIER (13) und REICHE (15) wurde unter anderem gepruft, inwieweit dieses Verfahren zur serologischen Differenzierung von Pseudomonadaceae, Ban'llaceae und Clostridium species eingesetzt werden kann. In folgenden Arbeiten werden die mit dieser Technik gemachten Erfahrungen und erzielten Ergebnisse mit Keimen der Gattung Clostrzdium zusammengefafit sowie mit denen von BATTY und WALKER (I), ELLNER und GREEN (7, 8) sowie POXTON und BYRNE (14) verglichen. Material und Methoden Verwendete Clostridien-StammeDie verwendeten Clostridium spp. sind in der Tabelle 1 zusammengefadt. Die Differenzierung der Feldstamme erfolgt mittels biochemischer und gaschromatographischer Verfahren. Herstellung der AntigeneDie Anziichtung des Zellmaterials erfolgt in Anaerobier-Bouillon nach SCHAEDLER. Die Kulturrohrchen werden bei 37°C solange anaerob bebrutet bis sich eine deutliche Trubung des Mediums bzw. ein grauweider Niederschlag am Boden des Rohrchens gebildet hat. Dann werden...

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Versuche zu einer serologischen Tumordiagnostik mittels sporenbildender Bakterien (I)

Mse

1970

Z Krebs-forsch

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SEROLOGICAL RELATIONSHIP BETWEEN CLOSTRIDIUM BIFERMENTANS AND CLOSTRIDIUM SORDELLII BASED UPON SOLUBLE ANTIGENS

Abstract: The problem of whether Clostridium sordellii should be assigned species status or be retained as a pathogenic variant of C. bifermentans is a perplexing one. Despite marked similarities in morphology, colony formation, and biochemical reactions (Clark and Hall,

Cited by 7 publications

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Taxonomy of Clostridium bifermentans and Clostridium sordellii III. Agglutinability of Heat-Resistant Substrains of Clostridium sordellii

Taxonomy of Clostridium bifermentans and Clostridium sordellii III. Agglutinability of Heat-Resistant Substrains of Clostridium sordellii

Untersuchungen über die Möglichkeit zur serologischen Differenzierung von Clostridium species mittels Enzym‐Immun‐Verfahren

Versuche zu einer serologischen Tumordiagnostik mittels sporenbildender Bakterien (I)

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