Serological Relationship between Galactans from Normal Bovine Lung and from Mycoplasma mycoides

Shifrine, M.; Gourlay, R. N.

doi:10.1038/208498a0

Cited by 24 publications

(5 citation statements)

References 8 publications

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“…munofluorescence and immunoelectrophoresis can be accounted for by specific antimycoplasma antibody or whether some is directed against other, possibly hostderived, antigens. We did not test extensively for autoimmunity in our mycoplasma-injected rabbits, although autoimmune phenomena are not uncommon in other mycoplasmal diseases (36)(37)(38)(39)(40)(41)(42)(43)(44)(45). We did, however, observe the specific attachment of rabbit Ig to cells in synovial frozen sections, suggesting that some RF activity was present which was not detected by serologic assay.…”

Section: Discussionmentioning

confidence: 90%

Chronic arthritis of rabbits induced by mycoplasmas II.antibody response and the deposition of immune complexes

Washburn

Cole

Ward

1980

Arthritis & Rheumatism

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Chronic mycoplasma-induced arthritis of rabbits was accompanied by a highly active humoral immune response. Peak antibody titers coincided with the "disappearance" of mycoplasmas from joint tissues at 7 weeks. High synovial fluid antibody titers and immunoglobulin-producing cells in frozen sections of synovial membrane suggested local antibody production. Persistence of granular deposits containing IgG and complement in synovium and cartilage may be a mechanism for continuing inflammation in the apparent absence of cultivable mycoplasmas.A single intraarticular injection of Mycoplasma arthritidis or Mycoplasma pulmonis readily induced a chronic arthritis in rabbits lasting for at least 1 year in many animals. The histologic, clinical, and microbiologic characteristics of this disease over a 1-year period were described in a previous communication (1). One of the most interesting observations made in this study was the apparent "disappearance" of mycoplasmas from joint tissues about 7 weeks after injection, despite the persistence of active arthritis. Although Mycoplasma hyorhinis induces a similar disease in swine

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Section: Discussionmentioning

confidence: 90%

Chronic arthritis of rabbits induced by mycoplasmas II.antibody response and the deposition of immune complexes

Washburn

Cole

Ward

1980

Arthritis & Rheumatism

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“…This clearly pointed towards a role of capsular polysaccharides in Mmm persistence and dissemination. Antigenic cross-reactivity between Mmm capsular galactan and the pneumogalactan isolated from bovine lung was proposed to be at the origin of autoimmune reactions [15] and thus to participate in the inflammation process. In addition, intravenous injection of free, extracellular EPS induced specific effects on the vascular system of the lung and respiratory system of cattle [16] and promoted persistent bacteremia in calves, when it preceded inoculation of an Mmm strain [17], [18].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Free Exopolysaccharides Secreted by Mycoplasma mycoides Subsp. mycoides

et al. 2013

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Contagious bovine pleuropneumonia is a severe respiratory disease of cattle that is caused by a bacterium of the Mycoplasma genus, namely Mycoplasma mycoides subsp. mycoides (Mmm). In the absence of classical virulence determinants, the pathogenicity of Mmm is thought to rely on intrinsic metabolic functions and specific components of the outer cell surface. One of these latter, the capsular polysaccharide galactan has been notably demonstrated to play a role in Mmm persistence and dissemination. The free exopolysaccharides (EPS), also produced by Mmm and shown to circulate in the blood stream of infected cattle, have received little attention so far. Indeed, their characterization has been hindered by the presence of polysaccharide contaminants in the complex mycoplasma culture medium. In this study, we developed a method to produce large quantities of EPS by transfer of mycoplasma cells from their complex broth to a chemically defined medium and subsequent purification. NMR analyses revealed that the purified, free EPS had an identical β(1−>6)-galactofuranosyl structure to that of capsular galactan. We then analyzed intraclonal Mmm variants that produce opaque/translucent colonies on agar. First, we demonstrated that colony opacity was related to the production of a capsule, as observed by electron microscopy. We then compared the EPS extracts and showed that the non-capsulated, translucent colony variants produced higher amounts of free EPS than the capsulated, opaque colony variants. This phenotypic variation was associated with an antigenic variation of a specific glucose phosphotransferase permease. Finally, we conducted in silico analyses of candidate polysaccharide biosynthetic pathways in order to decipher the potential link between glucose phosphotransferase permease activity and attachment/release of galactan. The co-existence of variants producing alternative forms of galactan (capsular versus free extracellular galactan) and associated with an antigenic switch constitutes a finely tuned mechanism that may be involved in virulence.

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“…My coplasma mycoides was shown by Shifrine & Gourlay (121) to induce anti lung antibodies. Shifrine & Moulton (122) suggested that these antibodies attach to sites in the lungs and cause tissue damage; they postulated that M mycoides attaches to the site in the lung that contains antibody.…”

Section: Mycoplasmamentioning

confidence: 93%

Antigens Common to Hosts and Parasites

DeVay¹,

Adler²

1976

Annu. Rev. Microbiol.

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Serological Relationship between Galactans from Normal Bovine Lung and from Mycoplasma mycoides

Cited by 24 publications

References 8 publications

Chronic arthritis of rabbits induced by mycoplasmas II.antibody response and the deposition of immune complexes

Chronic arthritis of rabbits induced by mycoplasmas II.antibody response and the deposition of immune complexes

Characterization of Free Exopolysaccharides Secreted by Mycoplasma mycoides Subsp. mycoides

Antigens Common to Hosts and Parasites

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