Serological relationship between cattle exposed to Brucella abortus, Yersinia enterocolitica O:9 and Escherichia coli O157:H7

Nielsen, Klaus; Smith, Phillip D.; Widdison, J; Gall, D.; Kelly, L.; Kelly, William A.; Nicoletti, P

doi:10.1016/j.vetmic.2003.12.010

Cited by 101 publications

(69 citation statements)

References 23 publications

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“…and a number of other microorganisms, primarily Yersinia enterocolitica O:9, which are difficult to distinguish from specific anti-Brucella reactions. Similarity of the OPS, the immunodominant region of the LPS molecule, is responsible for most of the observed cross reactions (5,13,22). Thus, quick and accurate diagnosis of porcine brucellosis is very important for an effective outcome of eradication and monitoring programmes and precise establishing of epidemiological status of herds of pigs and individual animals.…”

Section: Discussionmentioning

confidence: 99%

“…If a molecule is labelled, the rotation can be determined by measuring polarised light intensity in vertical and horizontal planes (19). It is host species-independent method and can be performed also with whole blood or milk (9,11,(20)(21)(22). According to OIE Terrestrial Manual 2009, the FPA is recommended as a prescribed test for bovine and porcine brucellosis for determining the health status of animals (19).…”

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confidence: 99%

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Value of Fluorescence Polarisation Assay in Comparison to Traditional Techniques in Diagnosis of Porcine Brucellosis

Weiner

Iwaniak

Szulowski

2012

Bulletin of the Veterinary Institute in Pulawy

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The aim of this study was the evaluation of fluorescence polarisation assay (FPA) in the diagnosis of porcine brucellosis in comparison with Rose Bengal test (RBT), serum agglutination test (SAT), complement fixation test (CFT), 2-mercaptoethanol test, and ELISA. Eight hundred seventeen sera from pigs, including 612 sera from healthy animals, seven sera from Brucella suis bv2 culture positive animals, and 198 sera classified as false positive, originated from confirmatory investigations, were used. All sera from healthy animals, negative in RBT, SAT, CFT, and ELISA were also negative in FPA. All sera positive in serological examination, originated from Brucella infected animals, were also positive in FPA. Among sera classified as false positive almost half of the samples tested (49.49%) reacted positively in FPA. The examinations confirmed the usefulness of FPA in diagnosis of porcine brucellosis, but the method, like the other tests, does not allow resolving the problem of discrimination cross-reacting from specific antibodies.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Value of Fluorescence Polarisation Assay in Comparison to Traditional Techniques in Diagnosis of Porcine Brucellosis

Weiner

Iwaniak

Szulowski

2012

Bulletin of the Veterinary Institute in Pulawy

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“…may cause false positive reactions. Therefore, the antibodies should be examined for cross-reactivity (Behan and Klein, 1982;Nielsen et al, 2004).…”

Section: Classical Immuno-assaysmentioning

confidence: 99%

Current and emerging assays for Francisella tularensis detection: a review

Pohanka¹,

Hubálek²,

Neubauerová³

et al. 2008

Vet. Med. - Czech

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This paper presents an overview of methods for detection and identification of the pathogenic bacterium Francisella tularensis such as cultivation tests, enzyme-linked immunosorbent assays, flow cytometry, polymerase chain reaction, immunosensor, microarray, mass spectrometry, and chromatography. Included references are chosen according to their practical importance or perspectives for the future.

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“…[8] Brucella LPS is a strong immunogen but its epitopes cross-reacts with other Gramnegative bacteria especially Yersinia enterocolitica O:9, Vibrio cholerae O:1, Escherichia coli O:157, Salmonella O:30, Francisella tularensis, etc., thereby increasing the rate of false positivity. [9,10] The Enzyme-linked immunosorbent assay (ELISA) measures immunoglobulin G, immunoglobulin M, and immunoglobulin A, which is sensitive and allows for a better interpretation of the clinical situation.…”

Section: Introductionmentioning

confidence: 99%

Missed opportunities for the diagnosis of Brucella infection among slaughterhouse workers at the Kumasi Abattoir, Ghana

Amegashie

Annan

Awuah

et al. 2017

JER

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Brucellosis is a zoonotic disease in humans with its public health importance. Laboratory diagnostic methods targeting brucellosis are not performed in hospital settings across Ghana. Very little is known about the comparative diagnostic abilities of the various tests available presently. The aim of this study therefore was to evaluate and compare diagnostic performances of Rose Bengal Plate Test (RBPT), Enzyme Linked Immunosorbent Assay (ELISA) and Polymerase Chain Reaction (PCR) employed in diagnosing Brucella infection. Two hundred and twenty Abattoir workers were randomly selected in Kumasi, Ghana. Blood samples were collected, serum extracted and tested for the presence of anti-Brucella antibodies and compared among three different techniques, using ELISA, RBPT and PCR. From the 220 participants tested for antibodies against Brucella spp., 3 (1.4%), 4 (1.8%) and 21 (9.6%) were positive for Rose Bengal Plate test, anti-Brucella ELISA IgM and anti-Brucella ELISA IgG respectively. A total of 98 (44.5%) participants tested positive by PCR. The sensitivity, specificity, positive predictive value, negative predictive values and Kappa value for Rose Bengal in comparison with PCR were 66.7%, 55.8%, 2.0%, 100% and 0.013 respectively while that for ELISA IgG in comparison with PCR were 85.7%, 71.3%, 18.4%, 98.5% and 0.212 respectively. PCR yielded the highest sensitivity and specificity among the three diagnostic methods in this study and should be considered for use at strategic reference laboratories to augment existing routine serological tests for brucella performed in laboratories in Ghana.

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Serological relationship between cattle exposed to Brucella abortus, Yersinia enterocolitica O:9 and Escherichia coli O157:H7

Cited by 101 publications

References 23 publications

Value of Fluorescence Polarisation Assay in Comparison to Traditional Techniques in Diagnosis of Porcine Brucellosis

Value of Fluorescence Polarisation Assay in Comparison to Traditional Techniques in Diagnosis of Porcine Brucellosis

Current and emerging assays for Francisella tularensis detection: a review

Missed opportunities for the diagnosis of Brucella infection among slaughterhouse workers at the Kumasi Abattoir, Ghana

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