Serological proteome analysis of Corynebacterium pseudotuberculosis isolated from different hosts reveals novel candidates for prophylactics to control caseous lymphadenitis

Seyffert, Núbia; Silva, Renata Faria; Jardin, Julien; Silva, Wanderson Marques; Castro, Thiago Luiz de Paula; Tartaglia, Natayme Rocha; Santana, Karina Talita de Oliveira; Portela, Ricardo Wagner; Silva, Artur M. S.; Miyoshi, Anderson; Loir, Yves Le; Azevedo, Vasco

doi:10.1016/j.vetmic.2014.08.024

Cited by 17 publications

(12 citation statements)

References 26 publications

(28 reference statements)

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“…In C. diphtheriae , it was demonstrated that a protein with trans-sialidase activity promotes cellular invasion [ 63 , 64 ]. In addition, NanH was reported to be immunoreactive in the immunoproteome of 1002_ ovis , showing the antigenicity of this protein [ 65 ]. Interestingly, genomic difference in relation to gene involved in the adhesion and invasion process, also already were observed between biovar ovis strain and biovar equi strains, mainly in genes related to pilus [ 10 , 12 ].…”

Section: Resultsmentioning

confidence: 99%

Label-free quantitative proteomics of Corynebacterium pseudotuberculosis isolates reveals differences between Biovars ovis and equi strains

et al. 2017

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Background Corynebacterium pseudotuberculosis is a pathogen classified into two biovars: C. pseudotuberculosis biovar ovis, the etiologic agent of caseous lymphadenitis and C. pseudotuberculosis biovar equi, which causes ulcerative lymphangitis. The available whole genome sequences of different C. pseudotuberculosis strains have enabled identify difference of genes related both virulence and physiology of each biovar. To evaluate be this difference could reflect at proteomic level and to better understand the shared factors and the exclusive ones of biovar ovis and biovar equi strains, we applied the label-free quantitative proteomic to characterize the proteome of the strains: 1002_ovis and 258_equi, isolated from goat (Brazil) and equine (Belgium), respectively.ResultsFrom this analysis, we characterized a total of 1230 proteins in 1002_ovis and 1220 in 258_equi with high confidence. Moreover, the core-proteome between 1002_ovis and 258_equi obtained here is composed of 1122 proteins involved in different cellular processes, which could be necessary for the free living of C. pseudotuberculosis. In addition, 120 proteins from this core-proteome presented change in abundant with statistically significant differences. Considering the exclusive proteome, we detected strain-specific proteins to each strain. When correlated, the exclusive proteome of each strain and proteome with change in abundant, the proteomic differences, between the 1002_ovis and 258_equi, this related to proteins involved in cellular metabolism, information storage and processing, cellular processes and signaling.ConclusionsThis study reports the first comparative proteomic study of the biovars ovis and equi of C. pseudotuberculosis. The results generated in this study provide information about factors which can contribute to understanding both the physiology and the virulence of this pathogen.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3835-y) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Label-free quantitative proteomics of Corynebacterium pseudotuberculosis isolates reveals differences between Biovars ovis and equi strains

et al. 2017

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“…The presence of these systems shows the versatility of 258_ equi in acquiring iron from different sources. Interestingly, the HtaA protein was detected to be immunoreactive in an immunoproteomic study of C. pseudotuberculosis biovar ovis (Seyffert et al, 2014 ). Taken together, these results indicate that, similar to other pathogens, iron acquisition likely plays an important role in 258_ equi virulence as this strain uses distinct iron acquisition systems during infection.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, putative antigenic proteins were identified through reverse vaccinology (Soares et al, 2013a ). Some studies have shown that extracellular proteins are related to the pathogenic process of C. pseudotuberculosis (Wilson et al, 1995 ; Billington et al, 2002 ; Pacheco et al, 2012 ; Seyffert et al, 2014 ). However, phospholipase D (Pld) exotoxin, which contributes to bacterial spread in the host, is considered the major virulence factor of this pathogen (McKean et al, 2007 ).…”

Section: Introductionmentioning

confidence: 99%

Quantitative Proteomic Analysis Reveals Changes in the Benchmark Corynebacterium pseudotuberculosis Biovar Equi Exoproteome after Passage in a Murine Host

Silva

Carvalho

Dorella

et al. 2017

Front. Cell. Infect. Microbiol.

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Corynebacterium pseudotuberculosis biovar equi is the etiologic agent of ulcerative lymphangitis. To investigate proteins that could be related to the virulence of this pathogen, we combined an experimental passage process using a murine model and high-throughput proteomics with a mass spectrometry, data-independent acquisition (LC-MSE) approach to identify and quantify the proteins released into the supernatants of strain 258_equi. To our knowledge, this approach allowed characterization of the exoproteome of a C. pseudotuberculosis equi strain for the first time. Interestingly, the recovery of this strain from infected mouse spleens induced a change in its virulence potential, and it became more virulent in a second infection challenge. Proteomic screening performed from culture supernatant of the control and recovered conditions revealed 104 proteins that were differentially expressed between the two conditions. In this context, proteomic analysis of the recovered condition detected the induction of proteins involved in bacterial pathogenesis, mainly related to iron uptake. In addition, KEGG enrichment analysis showed that ABC transporters, bacterial secretion systems and protein export pathways were significantly altered in the recovered condition. These findings show that secretion and secreted proteins are key elements in the virulence and adaptation of C. pseudotuberculosis. Collectively, bacterial pathogenesis-related proteins were identified that contribute to the processes of adherence, intracellular growth and evasion of the immune system. Moreover, this study enhances our understanding of the factors that may influence the pathogenesis of C. pseudotuberculosis.

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“…Esses estudos moleculares sofreram grande avanço após o sequenciamento do genoma de diversas cepas de C. pseudotuberculosis, e modelos vacinais baseados em bactérias recombinantes têm apresentado resultados promissores . Uma promessa tem sido a análise proteômica de proteínas reconhecidas por soros de pequenos ruminantes naturalmente infectados pela bactéria, a qual tem revelado novos alvos para o desenvolvimento de vacinas (Seyffert et al 2014). Apesar das pesquisas em vacinas estarem direcionadas, em sua grande parte, para pequenos ruminantes, as mesmas podem ser importantes para fundamentar novos antígenos a serem empregados na imunoprofilaxia da doença em equídeos.…”

Section: Prevençãounclassified

Infecção por Corynebacterium pseudotuberculosis em equinos: aspectos microbiológicos, clínicos e preventivos

et al. 2015

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Serological proteome analysis of Corynebacterium pseudotuberculosis isolated from different hosts reveals novel candidates for prophylactics to control caseous lymphadenitis

Cited by 17 publications

References 26 publications

Label-free quantitative proteomics of Corynebacterium pseudotuberculosis isolates reveals differences between Biovars ovis and equi strains

Label-free quantitative proteomics of Corynebacterium pseudotuberculosis isolates reveals differences between Biovars ovis and equi strains

Quantitative Proteomic Analysis Reveals Changes in the Benchmark Corynebacterium pseudotuberculosis Biovar Equi Exoproteome after Passage in a Murine Host

Infecção por Corynebacterium pseudotuberculosis em equinos: aspectos microbiológicos, clínicos e preventivos

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