Infecção por Corynebacterium pseudotuberculosis em equinos: aspectos microbiológicos, clínicos e preventivos

Guedes, Maria Tereza B.; Souza, Bianca Carla Azevedo de; Sousa, Thiago J.; Loureiro, Dan; Moura-Costa, Lília Ferreira de; Azevedo, Vasco; Meyer, Roberto; Portela, Ricardo Wagner

doi:10.1590/s0100-736x2015000800001

Cited by 5 publications

(8 citation statements)

References 67 publications

(84 reference statements)

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“…This disease continues to spread worldwide, not only in small ruminants but also with pathogenic relevance in horses (Barba et al, 2015;Boysen, Davis, Beard, Lubbers, & Raghavan, 2015;Guedes et al, 2015;Spier & Azevedo, 2017), cattle (Rocha, 2011;Shpigel, Elad, Yeruham, Winkler, & Saran, 1993;Smith Bradford, 2015), wild ungulates such as the Iberian ibex (Capra pyrenaica hispanica) (Colom-Cadena et al, 2014) and swine (Oliveira et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Utility assessment of an Enzyme‐linked immunosorbent assay for detection of subclinical cases of caseous lymphadenitis in small ruminant flocks

Costa

Huerta

Galán‐Relaño

et al. 2020

Veterinary Medicine & Sci

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The actual prevalence of CLA (caseous lymphadenitis) in small ruminant flocks is underestimated in many countries, and because it is not a notifiable disease, it will continue to spread without data and information about its real economic impact. The difficulty in the accurate identification of the causative agent in internal subclinical cases allows the disease to spread within and between flocks. This research intends to assess the utility of an ELISA (enzyme‐linked immunosorbent assay) test in the detection of internal subclinical cases of CLA in farms and to simultaneously add data on the seroprevalence of the disease in Portugal. Sera from 756 small ruminants, 70% sheep (528/756) and 30% goats (228/756) were screened for antibodies against Corynebacterium pseudotuberculosis using the ELISA technique based on a recombinant phospholipase D (ELITEST CLA # CK105A®). The animals showing internal lesions (n ꞊ 58) were sampled for the identification of the aetiological agent. In this investigation, the prevalence of CLA was 34% (258/756), with the ELISA test showing a low specificity (78%) and high sensitivity (100%). The proof was able to detect 57% (13/23) of subclinical cases of CLA confirmed by postmortem examination and conventional PCR (polymerase chain reaction). The results also reveal that goats have a higher propensity for the disease, and dairy farms and non‐extensive production units appear to be more susceptible to CLA. This research clarifies an actual problem and pointed out the importance of CLA in small ruminant herds in Portugal. Finally seems to demonstrate that the ELISA test is a good diagnostic tool for use in CLA eradication programmes.

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Section: Introductionmentioning

confidence: 99%

Utility assessment of an Enzyme‐linked immunosorbent assay for detection of subclinical cases of caseous lymphadenitis in small ruminant flocks

Costa

Huerta

Galán‐Relaño

et al. 2020

Veterinary Medicine & Sci

View full text Add to dashboard Cite

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“…As lesões são mais comuns nos membros posteriores, especialmente distais ao jarrete, com formação de nódulos dolorosos, edemaciados, os quais fistulizam, drenando pus com característica cremosa (Reed et al, 2009;Thomassian, 2006). Não existe predileção por idade, sexo ou raça (Guedes et al, 2015).…”

Section: Introductionunclassified

“…O tratamento varia de acordo com a manifestação clínica da doença (Guedes et al, 2015). Contudo, de modo geral, exercício, drenagem cirúrgica, higienização e proteção por bandagens da área e longo período de antibióticos fazem parte do protocolo terapêutico (Guedes et al, 2015;Mueller, 2007).…”

Section: Introductionunclassified

“…A antibioticoterapia consiste no uso de penicilina, tetraciclina e sulfa com trimetropim, sendo sugeridas em alguns estudos injeções parenterais de penicilina, tetraciclina ou penicilina G procaína, substâncias cujos agentes patogênicos se mostram sensíveis (Guedes et al, 2015;Radostits et al, 2010;Thomassian, 2005;Thomassian, 2006).…”

Section: Introductionunclassified

“…Bom a reservado em lesões iniciais; reservado a mal se fibrose grave estiver presente (Mueller, 2007). Existem métodos eficientes para prevenir a infecção, tais como: higiene dos estábulos, baias, pastos e picadeiros, controle constante de vetores, isolamento dos animais infectados, e evitar a aglomeração constante da população equina (Guedes et al, 2015;Radostits et al, 2010;Sureshjani et al, 2015).…”

Section: Introductionunclassified

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