Serological Identification of Enterotoxigenic Staphylococci from Cheese

Donnelly, C. B.; Leslie, J. E.; Black, L. A.; Lewis, Keith H.

doi:10.1128/am.15.6.1382-1387.1967

Cited by 55 publications

(6 citation statements)

References 15 publications

(4 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results presented in this paper show that the S. aureus strains examined most often produced enterotoxin A. Similar results have been obtained by workers in other countries (6,9,21,25). Of all the strains examined, 51% were toxigenic, and this result is also in agrement with other reports (4).…”

Section: Discussionsupporting

confidence: 91%

“…Micrococcal nuclease (Worthington Biochemical Corp., Freehold, N.J.) was used for preparation of a standard curve. The ability of each strain to produce thermonuclease was determined from the buffer solution of dialysis sac cultures incubated in Brain Heart Infusion (Difco) for 24 h at 37 C, as described by Donnelly et al (9). Before pipetting, samples were heated for 15 min at 100 C. Uninoculated buffer solution was used as negative control.…”

Section: Assay Ofthermonuc/easementioning

confidence: 99%

“…Phage-typing has been used in attempt to clarify the origin (human or animal) of staphylococci and the immediate source of contamination in cases of food poisoning. It has frequently been reported that toxigenic strains isolated in connection with food poisoning outbreaks have belonged to phage group III (9,23,28). Strains isolated from the nasal cavity, considered the most important reservoir of toxigenic staphylococci, have also mainly belonged to phage group III (17) while strains from udder infections have mostly belonged to group IV (13,26).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Correlation of Enterotoxin and Thermonuclease Production with Some Physiological and Biochemical Properties of Staphylococcal Strains Isolated From Different Sources

Niskanen

Koiranen

1977

Journal of Food Protection

View full text Add to dashboard Cite

A total of 276 Staphylococcus aureus strains isolated from routine sampling, food poisoning outbreaks, and mastitic milk were examined for production of enterotoxins A, B, C, D, and E. Quantitative determination of thermonuclease was carried out from the dialysis sac culture supernatant fluids obtained during the enterotoxin assay. The toxic properties of the strains were compared with other biochemical properties (coagulase, phosphate, production of pigment, and hemotoxin) and with their sensitivity to antibiotics and phages. Fifty one percent of the strains examined produced enterotoxin, and of the toxigenic strains 53% produced enterotoxin A, 4% produced B, 38% C, and 20% D. Production of enterotoxin E was not observed with any of the strains. Production of thermonuclease averaged 19.4 and 25.5 μg/ml for toxigenic and non-toxigenic strains, respectively. All toxigenic strains produced coagulase, 98% produced phosphatase, 92% hemotoxin, and 79% yellow or orange pigment. Of the toxigenic strains isolated from food, 56% belonged to phage group III, and of the toxigenic strains isolated from mastitis cases 31.4% belonged to phage group M and 21% to phage group III. Resistance to antibiotics was slightly more widespread among the toxigenic than the non-toxigenic strains.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Assay Ofthermonuc/easementioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Correlation of Enterotoxin and Thermonuclease Production with Some Physiological and Biochemical Properties of Staphylococcal Strains Isolated From Different Sources

Niskanen

Koiranen

1977

Journal of Food Protection

View full text Add to dashboard Cite

show abstract

“…Nuclease (DNAse) and thermonuclease (TNAse) were determined by the metachromatic Toluidine blue O agar diffusion-DNA technique according to Lachica et al [32]. Supernatants obtained by the sac culture method of Donnelly et al [33] as described below were transferred to the wells of plates containing metachromatic Toluidine blue O agar. The culture supernatant was first heated in a water bath for 20 min for the detection of TNAse.…”

Section: Methodsmentioning

confidence: 99%

“…For the evaluation of the production of enterotoxins and TSST-1, the sac culture method [33] was used to determine the toxigenic profile of the strains. Dialysis sacs filled with 50 ml doubleconcentrated BHI broth were placed in U-shaped Erlenmeyer flasks and autoclaved at 121uC for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Correction: Peritoneal Dialysis-Related Peritonitis Due to Staphylococcus aureus: A Single-Center Experience over 15 Years

Barretti¹,

Moraes²,

Camargo³

et al. 2012

PLoS ONE

View full text Add to dashboard Cite

Peritonitis caused by Staphylococcus aureus is a serious complication of peritoneal dialysis (PD), which is associated with poor outcome and high PD failure rates. We reviewed the records of 62 S. aureus peritonitis episodes that occurred between 1996 and 2010 in the dialysis unit of a single university hospital and evaluated the host and bacterial factors influencing peritonitis outcome. Peritonitis incidence was calculated for three subsequent 5-year periods and compared using a Poisson regression model. The production of biofilm, enzymes, and toxins was evaluated. Oxacillin resistance was evaluated based on minimum inhibitory concentration and presence of the mecA gene. Logistic regression was used for the analysis of demographic, clinical, and microbiological factors influencing peritonitis outcome. Resolution and death rates were compared with 117 contemporary coagulase-negative staphylococcus (CoNS) episodes. The incidence of S. aureus peritonitis declined significantly over time from 0.13 in 1996-2000 to 0.04 episodes/patient/year in 2006-2010 (p = 0.03). The oxacillin resistance rate was 11.3%. Toxin and enzyme production was expressive, except for enterotoxin D. Biofilm production was positive in 88.7% of strains. The presence of the mecA gene was associated with a higher frequency of fever and abdominal pain. The logistic regression model showed that diabetes mellitus (p = 0.009) and b-hemolysin production (p = 0.006) were independent predictors of non-resolution of infection. The probability of resolution was higher among patients aged 41 to 60 years than among those .60 years (p = 0.02). A trend to higher death rate was observed for S. aureus episodes (9.7%) compared to CoNS episodes (2.5%), (p = 0.08), whereas resolution rates were similar. Despite the decline in incidence, S. aureus peritonitis remains a serious complication of PD that is associated with a high death rate. The outcome of this infection is negatively influenced by host factors such as age and diabetes mellitus. In addition, b-hemolysin production is predictive of non-resolution of infection, suggesting a pathogenic role of this factor in PD-related S. aureus peritonitis.

show abstract

Specificity, cross-reactivity and competition profile of monoclonal antibodies to staphylococcal enterotoxins B and C1 detected by indirect enzyme-linked immunosorbent assays

Kienle

Buschmann

1989

Med Microbiol Immunol

View full text Add to dashboard Cite

A group of monoclonal antibodies to the staphylococcal enterotoxins B and C1 without any cross-reactivity to the other known staphylococcal enterotoxins A, C2, C3, D and E was developed. The monoclonal antibodies were compared in competition ELISA's with regard to affinity and epitope recognition. Three different groups could be classified: Group 1, consisting of the MAbs B/3-4 and B/3-8, recognizes in identical manner staphylococcal enterotoxin B. Also group 2 (MAb B/3-5) recognizes only staphylococcal enterotoxin B, yet group 1 and 2 do not compete with each other in the competition ELISA and therefore own different paratopes. Group 3 (MAb C1/2-3 and C1/4-6) reacts exclusively with staphylococcal enterotoxin C1, not even with staphylococcal enterotoxins C2 and C3.

show abstract

Serological Identification of Enterotoxigenic Staphylococci from Cheese

Cited by 55 publications

References 15 publications

Correlation of Enterotoxin and Thermonuclease Production with Some Physiological and Biochemical Properties of Staphylococcal Strains Isolated From Different Sources

Correlation of Enterotoxin and Thermonuclease Production with Some Physiological and Biochemical Properties of Staphylococcal Strains Isolated From Different Sources

Correction: Peritoneal Dialysis-Related Peritonitis Due to Staphylococcus aureus: A Single-Center Experience over 15 Years

Specificity, cross-reactivity and competition profile of monoclonal antibodies to staphylococcal enterotoxins B and C1 detected by indirect enzyme-linked immunosorbent assays

Contact Info

Product

Resources

About