Serological Evidence of Thailand Orthohantavirus or Antigenically Related Virus Infection Among Rodents in a Chronic Kidney Disease of Unknown Etiology Endemic Area, Girandurukotte, Sri Lanka

Lokupathirage, Sithumini M. W.; Muthusinghe, Devinda S.; Shimizu, Kenta; Nishigami, Kumpei; Noda, Kisho; Tsuda, Yuichi; Sarathkumara, Yomani D.; Gunawardana, Sirimevan; Arikawa, Jiro; Gamage, Chandika D.; Yoshimatsu, Kumiko

doi:10.1089/vbz.2018.2429

Cited by 8 publications

(7 citation statements)

References 38 publications

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“…Morphological identification showed that the most (99/116) of the captured small mammals were Rattus rattus . An analysis of the cytb sequences from several animals confirmed that they belonged to lineage Ib, a Sri Lankan endemic lineage of R. rattus [ 25 , 28 ]. Eleven animals were identified as Mus booduga (Little Indian field mouse) after analyzing the cytb sequences ( Supplemental Figure S1 ).…”

Section: Resultsmentioning

confidence: 99%

“…Initially, rats were the targets of trapping, and we used only cage-type traps. As a result, many seropositive rats were captured, but a virus genome was not identified from any of them [ 25 ]. A partial Lanka virus genome was first detected from a seronegative rat (#32) after heminested PCR.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, a cross-sectional study carried out with case-control comparisons in two geographically distinct CKDu endemic areas vs. a nonendemic area in Sri Lanka demonstrated that exposure to the hantavirus was an independent risk factor associated with renal disease in the CKDu endemic regions [ 24 ]. An ecoepidemiological study in Girandurukotte serologically confirmed that THAIV-like hantavirus species were highly prevalent among the Rattus rattus lineage [ 25 ]. Serological findings from both humans and rodents in the CKDu areas supported the hypothesis that exposure to hantaviruses is a risk factor for the possible development of CKDu in Sri Lanka [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

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Identification of Novel Rodent-Borne Orthohantaviruses in an Endemic Area of Chronic Kidney Disease of Unknown Etiology (CKDu) in Sri Lanka

Muthusinghe

Shimizu

Lokupathirage

et al. 2021

Viruses

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We reported the genetic evidence of circulating hantaviruses from small mammals captured in a chronic kidney disease of unknown etiology (CKDu) hotspot area of Sri Lanka. The high seroprevalence of anti-hantavirus antibodies against Thailand orthohantavirus (THAIV) has been reported among CKDu patients and rodents in Sri Lankan CKDu hotspots. We captured 116 small mammals from CKDu endemic regions in the Polonnaruwa District of Sri Lanka. Seven animals (five out of 11 Mus booduga and two out of 99 Rattus rattus) were PCR-positive for the hantavirus. A rat-borne sequence was grouped with a THAIV-like Anjozorobe virus. In contrast, Mus-borne sequences belonged to the THAIV lineage, suggesting a novel orthohantavirus species according to the phylogenetic analyses and whole-genome comparisons. Our genetic evidence indicates the presence of two THAIV-related viruses circulating in this CKDu endemic area, suggesting a basis for further investigations to identify the infectious virus in patients with CKDu and the CKDu induction mechanism of these viruses.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification of Novel Rodent-Borne Orthohantaviruses in an Endemic Area of Chronic Kidney Disease of Unknown Etiology (CKDu) in Sri Lanka

Muthusinghe

Shimizu

Lokupathirage

et al. 2021

Viruses

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“…A recombinant NP antigen of THAIV was originally prepared for serological analyses of rodent sera [15]. The amplified NP gene tagged with ClaI and XhoI recognition sites was cloned into a mammalian expression plasmid vector, pCAGGS/MCS following ClaI and XhoI restriction treatment of the NP amplicons and pCAGGS/MCS.…”

Section: Methodsmentioning

confidence: 99%

Exposure to Hantavirus is a Risk Factor Associated with Kidney Diseases in Sri Lanka: A Cross Sectional Study

Sarathkumara

Gamage

Lokupathirage

et al. 2019

Viruses

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Chronic kidney disease of unknown etiology (CKDu) imposes a substantial burden on public health in Sri Lankan agricultural communities. High seroprevalences of hantavirus have been reported in CKDu patients in several locations of Sri Lanka. We carried out a cross-sectional study followed by an unmatched case-control comparison in two geographically distinct areas of Sri Lanka, Girandurukotte (CKDu endemic) and Kandy (CKDu non-endemic) to determine whether exposure to hantaviruses is a potential risk factor in patients with kidney disease. An indirect immunofluorescent antibody assay using two antigens, Thailand orthohantavirus-infected and recombinant N protein-expressing Vero E6 cells, were used for serodiagnosis. Participants’ demographic and other socio-economic data were collected through a structured questionnaire. Fifty kidney disease patients and 270 controls from Kandy and 104 kidney disease patients and 242 controls from Girandurukotte were examined. Seropositivities were 50% and 17.4% in kidney patients and controls, respectively, in Girandurukotte, and they were 18% and 7% in Kandy. The odds of exposure to hantaviruses were higher for kidney disease patients than for controls in both Girandurukotte (OR:3.66, 95% CI:2.01 to 6.64) and Kandy (OR:2.64, 95% CI:1.07 to 6.54) in binary logistic regression models. According to statistical analysis, individuals exposed to hantaviruses had a higher risk of developing renal impairment. Therefore, hantavirus infection might be an important risk factor for development of kidney disease in Sri Lanka.

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“…Briefly, BHK-21 cells were inoculated with DPRV. After 4 days of inoculation, cells were harvested and fixed on glass slides with paraformaldehyde as described previously ( 40 ). Serum samples were 2-fold serially diluted from 1:10 to 1:40 prior to inoculation with infected BHK-21 cells.…”

Section: Methodsmentioning

confidence: 99%

Isolation of a Novel Bat Rhabdovirus with Evidence of Human Exposure in China

et al. 2022

mBio

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Serological Evidence of Thailand Orthohantavirus or Antigenically Related Virus Infection Among Rodents in a Chronic Kidney Disease of Unknown Etiology Endemic Area, Girandurukotte, Sri Lanka

Cited by 8 publications

References 38 publications

Identification of Novel Rodent-Borne Orthohantaviruses in an Endemic Area of Chronic Kidney Disease of Unknown Etiology (CKDu) in Sri Lanka

Identification of Novel Rodent-Borne Orthohantaviruses in an Endemic Area of Chronic Kidney Disease of Unknown Etiology (CKDu) in Sri Lanka

Exposure to Hantavirus is a Risk Factor Associated with Kidney Diseases in Sri Lanka: A Cross Sectional Study

Isolation of a Novel Bat Rhabdovirus with Evidence of Human Exposure in China

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