Serological Evidence for the Association of Papillomavirus and Cervical Neoplasia

Use of human papillomavirus type 11 virions in an ELISA to detect specific antibodies in humans with condylomata acuminata

“…The antigenic specificity of HPV-1 ! viral particles that is apparent in our observation is not surprising: HPV-I virions have the same specificity also (Kienzler et , 1983;Anisimov~t et al, 1990;Steele & Gallimore, 1990;Viac et al, 1990). However, the limits and mechanisms of the antigenic specificity of viral particles of different HPV types will have to be more precisely defined.…”

“…However, these results were not correlated with HPV type or disease status. Other investigators utilized denatured bovine papillomavirus (BPV) type 2 in ELISA and detected antibodies among condylomata acuminata patients with an overall accuracy up to 99~ (Baird, 1983;Beiss et al, 1987). As denatured BPV exhibits a broadly cross-reactive papillomavirus antigen (Jenson et al, 1980), the specificity of these immunoassays for condylomata acuminata is surprising, and we have not been able to replicate these results (unpublished data).…”

Comparison of ELISA and Western blotting for human papillomavirus type 16 E7 antibody determination

“…In the past few years increasing efforts have been made to develop serological tests capable of proving HPV infection. In these tests, three sources of antigens have been utilized: (i) intact or disrupted HPV virions extracted either from HPVinduced lesions (Pfister & zur Hausen, 1978;Baird, 1983;Dillner et al, 1989a;Steele & Gallimore, 1990;Anisimov/t et al, 1990) or from virus-infected xenografts in nude mice (Christensen & Kreider, 1990), (ii) genetically engineered HPV proteins, usually in the form of bacterial fusion proteins (Jenison et al, 1988(Jenison et al, , 1989Jochmus-Kudielka et al, 1989) or expressed as part of a capsid protein of genetically modified phage fd (Mfiller et al, 1990) and (iii) synthetic peptides derived from known sequences of different HPV DNA open reading frames (ORFs) (Cason et al, 1989;Dillner, 1990;Dillner et al, 1989bDillner et al, , 1990Such~.nkov/~ et al, 1990;Miiller et al, 1990).…”

Time trends in prevalence of cervical cytological abnormality in women attending a sexually transmitted diseases clinic and their relationship to trends in sexual activity and specific infections

“…The attribution of causality to observed trends in an ecological analysis such as this is difficult, especially when the time span covered by the data is only short. While, on the evidence, both herpes virus infection and smoking are candidate explanations for the apparent trend in CIN, and both have been related to CIN and invasive cervical cancer in studies of individuals (Winkelstein et al, 1984;Baird, 1983), they can be taken no further than that in this study. Indeed, it might be argued that the trend observed in herpes virus infection might be more relevant to trends in CIN occurring in the succeeding rather than the same 5-year period given the likely latent interval between infection and onset of cytological change.…”

“…The trend in smoking, however, had been present at least since 1974 (as shown by intermediate observations in 1976(Gray & Hill, 1977Hill & Gray, 1982)). It might also be argued that the trend in evidence of wart virus infection, for which there is also evidence of a role in aetiology of cervical neoplasia (Baird, 1983), between 1978 and 1982 is irrelevant as the relevant period would be 5 years earlier; a period for which we have no data. Attribution of causality, therefore, must remain uncertain.…”