Serological evaluation of viral infections in bovine respiratory tract

Yavru, Sibel; Şimşek, Atilla; Yapkic, O.; Kale, Mehmet

doi:10.2298/avb0503219y

Cited by 22 publications

(2 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The individual seropositivity (59.78%) obtained in this study was in accordance with previous studies that reported seropositivity of BRSV in cattle ranged between 46.06% and 73% in Turkey (Yavru et al 2005 ; Yeşilbağ and Güngör, 2008 ; Kadiroğlu et al 2020 ). The highest seropositivity (73%) was found in Marmara region of Turkey.…”

Section: Discussionsupporting

confidence: 92%

“…There is limited knowledge available about the prevalence of BRSV infection (Yavru et al 2005 ; Yeşilbağ and Güngö, 2008 ; Kadiroğlu et al 2020 ) and genetic features of BRSV strains circulating in Turkey (Hacıoğlu et al 2019 ; Timurkan et al 2019 ; Yazici et al 2020 ). Furthermore, the possible risk factors for BRSV infection in cattle have not been studied in Turkey.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Risk factors and genetic characterization of bovine respiratory syncytial virus in the inner Aegean Region, Turkey

İnce

Şevik

Ozgur

et al. 2021

Trop Anim Health Prod

View full text Add to dashboard Cite

Bovine respiratory syncytial virus (BRSV) is one of the causative viral agents of the bovine respiratory disease complex. This study was conducted to determine the seropositivity and risk factors associated with BRSV infection and to evaluate the phylogenetic relatedness of the BRSVs in the inner Aegean region of Turkey. In this cross-sectional study, serum samples ( n = 557) and nasal swabs ( n = 21) were collected from cattle herds ( n = 43) between February 2018 and March 2019. A commercial indirect-ELISA kit was used for the detection of antibodies in the sera samples. Reverse-transcriptase PCR was used to detect viral RNA in nasal swabs. Nasal samples were also examined for the detection of bovine parainfluenza-3, bovine viral diarrhoea virus, and bovine herpesvirus 1 by molecular detection methods. Genetic characterization of the local BRSV field isolates was conducted by sequencing attachment glycoprotein (G) gene segment. Epidemiological data on potential risk factors were collected from each sampled herd during blood collection. All herds had at least one seropositive animal. After adjustment for assay sensitivity and specificity, the overall true seropositivity was 58.48% (95% CI: 53.32–63.47). BRSV RNA was detected in 2 of the 21 nasal swabs, whereas other infectious agents were not detected in the investigated samples. Phylogenetic analysis showed that the field isolates of BRSV obtained in this study belonged to subgroup III, but they were located on separate branch from previously characterised Turkish subgroup III isolates. BRSV field strains from this study displayed 3 new amino acid substitutions (P89S, D115G, and S165L) in the G protein chains compared to other main reference BRSV isolates, demonstrating that BRSV is still evolving. Generalised estimating equation model showed that there were positive associations between BRSV infection, age (OR = 2.36, p = 0.001), herd size (OR = 10.32, p < 0.001), herd type (OR = 8.97, p < 0.001), a past history of respiratory disease (OR = 4.06, p < 0.001). The results of this study revealed that BRSV infection is common among cattle herds in the inner Aegean region of Turkey. The obtained epidemiological and genetic data on BRSV infection from this study could be beneficial for designing effective biosecurity practices and vaccination strategies.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Risk factors and genetic characterization of bovine respiratory syncytial virus in the inner Aegean Region, Turkey

İnce

Şevik

Ozgur

et al. 2021

Trop Anim Health Prod

View full text Add to dashboard Cite

show abstract

A serosurvey for bovine respirovirus 3 in Turkish domestic ruminants: The first comparison study of A and C genotypes

Müftüoğlu

Kuruçay

Elhag

et al. 2021

Veterinary Medicine & Sci

View full text Add to dashboard Cite

Bovine parainfluenza virus‐3 (BPIV‐3), also known as bovine respirovirus 3, causes serious respiratory infection in ungulates, often involving other pathogens, such as viruses, bacteria and mycoplasmas. In this study, we evaluated antibody titers against virus genotypes A (BPIV‐3a) and C (BPIV‐3c). We conducted a serological survey and comparison analysis of archived serum samples from small and large ruminants reared in four Turkish provinces. A total of 1,307 samples, consisting of sheep (n = 444), cattle (n = 402), water buffalo (n = 261) and goat (n = 200) sera, were randomly selected from stock samples collected between 2015 and 2019 and screened by standard virus neutralisation assay. We found that 49.9% (653/1307) of all samples were positive for neutralising antibody titers. Goats had the highest titer, with total seropositivity of 63% (126/200), followed in descending order by cattle, sheep and water buffalo at 56.2% (226/402), 32.2% (143/444) and 26% (68/261) total seropositivity, respectively. BPIV‐3c had the highest neutralising antibody rate at 34.3% (448/1307), whereas BPIV‐3a had a 24.3% (317/1307) seropositivity rate. Neutralising antibody titers for positive samples ranged between 1/4 and 1/512 per the SN50 test. Seropositivity rates ranged from a low of 8.9% to a high of 18.3%. Our study was the first to compare antibody seroprevalence for two BPIV‐3 genotypes in small and large domestic ruminants, which were shown to be more commonly exposed to BPIV‐3c than BPIV‐3a. This finding could have significant implications as current vaccines mainly use the BPIV‐3a genotype. Further research can determine if current vaccines protect against different BPIV‐3 virus genotypes.

show abstract

Seroprevalence of bovine respiratory viruses in North-Western Turkey

Yeşilbağ

Güngör

2007

Trop Anim Health Prod

View full text Add to dashboard Cite

Bovine respiratory disease complex is a very important health problem around the world. Present study describes serological distribution of bovine major respiratory viruses in non-vaccinated cattle population of Marmara region in north-western Turkey. Neutralising antibodies specific to bovine viral diarrhoea virus (BVDV), bovine herpesvirus 1 (BHV-1), bovine respiratory syncytial virus (BRSV), bovine parainfluenza virus 3 (PI-3), bovine adenovirus serotype 1 (BAV-1) and serotype 3 (BAV-3) were investigated. Among 584 serum samples collected from 39 establishments in 7 provinces, 41.4% were positive for BVDV, 17.1% for BHV-1, 73.0% for BRSV, 43.0% for PI-3, 89.5% for BAV-1 and 92.3% for BAV-3. There were significant differences observed between seroprevalence rates detected in neighbouring provinces. Serological prevalence of BVDV, BHV-1 and BRSV were extremely higher in large capacity dairy farms than of small capacity farms (p<0.0001). This study demonstrates that herd capacity is a very important risk factor for respiratory viruses and, on the other hand bovine adenoviruses and BRSV are the common reason of respiratory diseases in the region.

show abstract

Serological evaluation of viral infections in bovine respiratory tract

Cited by 22 publications

References 4 publications

Risk factors and genetic characterization of bovine respiratory syncytial virus in the inner Aegean Region, Turkey

Risk factors and genetic characterization of bovine respiratory syncytial virus in the inner Aegean Region, Turkey

A serosurvey for bovine respirovirus 3 in Turkish domestic ruminants: The first comparison study of A and C genotypes

Seroprevalence of bovine respiratory viruses in North-Western Turkey

Contact Info

Product

Resources

About