Serological Diagnosis of Neosporosis in a Herd of Dairy Cattle in Southern Brazil

Locatelli-Dittrich, Rosângela; Soccol, V. T.; Richartz, R. R. T. B.; Gasino-Joineau, M. E.; Vinne, R.; Pinckney, R. D.

doi:10.2307/3285330

Cited by 12 publications

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“…Neosporosis has been described as an important cause of abortion in southern Brazil (CORBELLINI et al, 2001(CORBELLINI et al, , 2002LOCATELLI-DITTRICH et al, 2001). Locatelli-Dittrich et al (2001) reported that the abortion risk in N. caninum-seropositive dairy cows was four times higher than in seronegative dairy cows.…”

Section: Introductionmentioning

confidence: 99%

“…Locatelli-Dittrich et al (2001) reported that the abortion risk in N. caninum-seropositive dairy cows was four times higher than in seronegative dairy cows. Corbellini et al (2002) used both histopathological and serum sample examination from bovine aborted fetuses, and described a high rate of occurrence of N. caninum as the cause of abortion throughout the state of Rio Grande do Sul.…”

Section: Introductionmentioning

confidence: 99%

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Neospora caninum: evaluation of vertical transmission in slaughtered dairy cows (Bos taurus)

Macedo

Cardim

et al. 2013

Rev. Bras. Parasitol. Vet.

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Neospora caninum is a worldwide parasite recognized as one of the main parasites responsible for abortion in cattle. The objective of this study was to evaluate vertical transmission of N. caninum in dairy cows (Bos taurus) that were slaughtered at an abattoir in the state of Santa Catarina, southern Brazil. Blood samples (with and without EDTA) from 60 pregnant dairy cows and blood and tissue samples (brain, lung, heart and liver) from their fetuses were collected and used for PCR and serological evaluation. Blood samples from 60 non-pregnant cows were collected and used to detect antibodies. Anti-N. caninum antibodies were detected by indirect ELISA. Antibodies against N. caninum were observed in 41.6% (25/60) of the pregnant cows and in 43.3% (26/60) of the non-pregnant cows. Antibodies against the parasite were detected in sera from three fetuses (5.5%). PCR analysis revealed that 3.3% (2/60) of the cows and 6.6% (4/60) of the fetuses evaluated were positive for specific N. caninum primers. These positive fetuses were between 4-6 months of age. Therefore, considering PCR and serological tests to be indicative of vertical transmission in fetuses, 11.6% (7/60) of the fetuses were infected by N. caninum during gestation.Keywords: Neospora caninum, dairy cattle, vertical transmission, Bos taurus. ResumoNeospora caninum é um parasita de distribuição mundial reconhecido como um dentre os principais parasitas, responsável por abortamento em bovinos. O objetivo deste estudo foi avaliar a transmissão vertical de N. caninum em vacas leiteiras (Bos taurus) que foram submetidas ao abate em matadouro no Estado de Santa Catarina, sul do Brasil. Sangue (com e sem EDTA) de 60 vacas leiteiras prenhas e amostras de sangue e tecidos (cérebro, pulmão, coração e fígado) de seus fetos foram coletados e utilizados para PCR e avaliação sorológica. Amostras de sangue de 60 vacas não-gestantes foram obtidas e utilizadas para detecção de anticorpos. A detecção de anticorpos séricos anti-N. caninum foi avaliada pelo ELISA-teste indireto. Anticorpos anti-N. caninum foram observados em 41,6% (25/60) das vacas prenhas e em 43,3% (26/60) das vacas não-gestantes. Três fetos (5,5%) foram soros positivos para N. caninum. Análise pela PCR revelou que 3,3% (2/60) das vacas e 6,6% (4/60) dos fetos avaliados foram positivos para N. caninum. As idades dos fetos positivos eram de 4 a 6 meses. Portanto, considerando a PCR e a sorologia como indicativo de transmissão vertical em fetos, 11,6% (7/60) dos fetos foram infectados por N. caninum durante a gestação.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Neospora caninum: evaluation of vertical transmission in slaughtered dairy cows (Bos taurus)

Macedo

Cardim

et al. 2013

Rev. Bras. Parasitol. Vet.

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“…However, most of these reports were provisional and not a case controlled study. In PR, samples from a herd collected over a nine-yr follow-up period were analyzed for Neospora infections (LOCATELLI-DITTRICH et al, 2001). They found that the proportion of abortions was 20% and 8%, respectively for the seropositive and seronegative animals.…”

Section: Clinical Infectionsmentioning

confidence: 99%

All about neosporosis in Brazil

Cerqueira‐Cézar

Calero‐Bernal

Dubey

et al. 2017

Rev. Bras. Parasitol. Vet.

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Neospora caninum is protozoan parasite with domestic and wild dogs, coyotes and grey wolves as the definitive hosts and many warm-blooded animals as intermediate hosts. It was cultivated and named in 1988. Neosporosis is a major disease of cattle and has no public health significance. Since 1990's N. caninum has emerged as a major cause of abortion in cattle worldwide, including in Brazil. N. caninum also causes clinical infections in several other animal species. Considerable progress has been made in understanding the biology of N. caninum and there are more than 200 papers on this subject from Brazil. However, most of the reports on neosporosis from Brazil are serological surveys. Overall, little is known of clinical neosporosis in Brazil, particularly cattle. The few reports pertain to sporadic cases of abortion with no information on epidemics or storms of abortion. The objective of the present review is to summarize all reports from Brazil and suggest topic for further research, including prevalence of N. caninum oocysts in soil or in canine feces, and determining if there are additional definitive hosts, other than the domestic dog. There is need for a national survey in cattle using defined parameters. Future researches should focus on molecular characterization of N. caninum strains, possibility of vaccine production and relationship between wildlife and livestock epidemiology.Keywords: Neospora caninum, neosporosis, domestic animals, wild animals, Brazil. ResumoNeospora caninum é um protozoário parasita que possui os canídeos domésticos e selvagens, coiotes e lobos cinzentos como hospedeiros definitivos e vários animais de sangue quente como hospedeiros intermediários. Foi cultivado e nomeado em 1988. A neosporose é uma das principais doenças em bovinos e não tem significância em saúde pública. Desde 1990, N. caninum tem emergido como uma das principais causas de aborto em bovinos em todo o mundo, inclusive no Brasil. N. caninum também causa infecções clínicas em várias outras espécies animais. Consideráveis avanços foram feitos na compreensão da biologia desse parasita e há mais de 200 trabalhos sobre o assunto no Brasil. No entanto, a maioria dos relatos de neosporose do Brasil são relacionados a sorologia. Em geral, pouco se sabe sobre a neosporose clínica no Brasil, particularmente em bovinos. Os poucos relatos referem-se a casos esporádicos de aborto sem informações sobre epidemias ou surtos de aborto. O objetivo da presente revisão é resumir todos os relatos sobre N. caninum no Brasil e sugerir tópicos para pesquisas futuras, incluindo a prevalência de oocistos de N. caninum no solo ou em fezes caninas e determinar se há hospedeiros definitivos adicionais, exceto o cão doméstico no país. Uma pesquisa nacional em bovinos usando parâmetros definidos seria de grande importância. Pesquisas futuras deveriam ser focadas na caracterização de cepas de N. caninum, possibilidade de produção de vacinas e a relação epidemiológica entre a vida selvagem e o gado.Palavras-chave: Neospora caninum, neospo...

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“…Brazilian studies have contributed to the knowledge of distribution of infection in cows (Gennari 2004), its association with cases of abortion (Locatelli-Dittrich et al 2001, Corbellini et al 2002, its risk factors (Melo et al 2001, Guimarães Júnior et al 2004, and the isolation of the parasite (Gondim et al 2001) . However, there is much more to be investigated, since Brazilian territory has a wide climate variation with many different ecosystems, what implicates a great diversity in the breed population according to the geographic areas which could affect the distribution of neosporosis.…”

Section: Introductionmentioning

confidence: 99%

Neospora caninum seropositivity in cattle breeds in the South Fluminense Paraíba Valley, state of Rio de Janeiro

et al. 2009

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RESUMO.-[Soropositividade para Neospora caninum e raças bovinas no vale do Paraíba sulfluminense, Estado do Rio de Janeiro.] A realização deste estudo teve como objetivo verificar a ocorrência diferenças na distribuição da infecção por Neospora caninum em bovinos leiteiros da raça Holandesa Preta e Branca e mestiços na Mesorregião Fluminense a maior bacia leiteira do Rio de Janeiro. De um total de 2.491 vacas foram coletadas amostras de sangue de 563 de forma não randomizada, pertencentes a 57 propriedades localizadas nos municípios de Resende e Rio Claro. O tamanho da amostra por propriedade foi proporcional ao número de animais alvo da mesma. O abortamento ou distúrbios reprodutivos não foram utilizados como critérios de seleção. Foi verificada associação altamente significativa entre a soropositividade e o padrão racial (p=0,006). Ao conduzir as análises entre apenas dois padrões verificou-se os seguintes resultados: HPB versus zebuínos (p=0,0028) com 2,65 mais chances de sorpositividade em bovinos HPB; HPB versus (zebuínos + Gir x Holandês) p=0,01, com 2,23 mais chances de sopositividade também na raça HPB. Não houve diferença significativa entre HPB versus Gir x Holandês (p=0,08) e entre os zebuínos e Gir x Holandês (p=0,11). Este estudo suporta a hipótese que existe uma estreita relação entre a presença da infecção e a The infection by Neospora caninum of different cattle breeds in dairy properties in two municipalities of the South Fluminense Paraíba Valley, state of Rio de Janeiro, was evaluated. Considering a sampling universe of 2,491 cows, blood samples were collected from 563 dairy cows in 57 farms, which were randomically selected in proportion to the number of animals, using a random stratified sampling system. For each property the number of selected cows was proportional to the herd size. Abortion or other reproductive disorders were not considered as criteria for selecting the animals, and seropositivity was determined by indirect enzyme-linked immunosorbent assay (ELISA). A high association (p=0.006) between seropositivity and racial patterns was found. The analysis revealed that in comparison of pure black-and-white Holstein cows versus Zebu (p=0.0028), Holstein cows showed 2.65 times greater odds for seropositivity. In the same way, by comparing black-and-white Holstein versus Zebu + crossbreed Zebu/Holstein (p=0.01), it was noted that there is 2.23 times more chance for seropositivity in Holstein cattle. There were no significant differences concerning the comparison of Holstein cattle versus crossbreed Zebu/Holstein (p=0.08) or Zebu versus crossbreed Zebu/Holstein (p=0.11). This study supports the hypothesis that there is a close association between cattle breeds and the frequency of infection by N. caninum.

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Serological Diagnosis of Neosporosis in a Herd of Dairy Cattle in Southern Brazil

Cited by 12 publications

References 0 publications

Neospora caninum: evaluation of vertical transmission in slaughtered dairy cows (Bos taurus)

Neospora caninum: evaluation of vertical transmission in slaughtered dairy cows (Bos taurus)

All about neosporosis in Brazil

Neospora caninum seropositivity in cattle breeds in the South Fluminense Paraíba Valley, state of Rio de Janeiro

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