Serological cross-reactivity between Strongyloides venezuelensis and Syphacia muris in Wistar rats (Rattus norvegicus)

Sousa, José Eduardo Neto de; Carvalho, Edson Fernando Goulart de; Levenhagen, Marcelo Arantes; Faria, Lucas Silva de; Gonçalves-Pires, Maria do Rosário F.; Costa-Cruz, Júlia Maria

doi:10.1016/j.parint.2015.11.005

Cited by 6 publications

(4 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The 30-40 kDa band was recognized in the acute and recovery phases of infection in both groups. In agreement with previous reports [10][11][12] , this mass range has been considered an immunogenic band in experimental strongyloidiasis. In addition, 30-40 kDa bands are considered important for the diagnosis of S. stercoralis infections 6,13,14 .…”

supporting

confidence: 92%

“…Few studies have evaluated immunogenic bands during experimental infection [10][11][12] . In the present study, immunogenic bands were recognized (Figure 2), independent of the antigenic fraction and the phase of infection.…”

mentioning

confidence: 99%

“…In a recent study of experimental strongyloidiasis 11 , immunoreactive bands at 17, 38 and 50 kDa were demonstrated using S. venezuelensis iL3 alkaline extract. In addition, 36, 68, 76, 83 and 102 kDa bands for a saline extract were considered antigenic in another experimental investigation with S. venezuelensis 12 .…”

mentioning

confidence: 99%

See 2 more Smart Citations

Immunoreactivity of proteins within 30-40 kDa range during the acute and the recovery phases in rats experimentally infected with Strongyloides venezuelensis

Fonseca

Corral

Meisel

et al. 2020

Rev. Inst. Med. trop. S. Paulo

View full text Add to dashboard Cite

show abstract

supporting

confidence: 92%

mentioning

confidence: 99%

See 1 more Smart Citation

Immunoreactivity of proteins within 30-40 kDa range during the acute and the recovery phases in rats experimentally infected with Strongyloides venezuelensis

Fonseca

Corral

Meisel

et al. 2020

Rev. Inst. Med. trop. S. Paulo

View full text Add to dashboard Cite

show abstract

“…Serological tests for tissue invasive helminths are known to show a certain degree of cross-reactivity with antibodies against other helminth antigens which negatively affects assay specificity [ 31 – 33 ]. According to the manufacturer of the ICT IgG-IgM POC test, possible cross-reactivity with T .…”

Section: Discussionmentioning

confidence: 99%

Performance of a rapid immuno-chromatographic test (Schistosoma ICT IgG-IgM) for detecting Schistosoma-specific antibodies in sera of endemic and non-endemic populations

et al. 2022

View full text Add to dashboard Cite

Background Schistosomiasis, an acute and chronic parasitic disease caused by human pathogenic Schistosoma species, is a neglected tropical disease affecting more than 220 million people worldwide. For diagnosis of schistosomiasis, stool and urine microscopy for egg detection is still the recommended method, however sensitivity of these methods is limited. Therefore, other methods like molecular detection of DNA in stool, detection of circulating cathodic antigen in urine or circulating anodic antigen in urine and serum, as well as serological tests have gained more attention. This study examines the sensitivity and specificity of a rapid diagnostic test based on immunochromatography (Schistosoma ICT IgG-IgM, LD Bio, Lyon, France) for simultaneous detection of specific IgG and IgM antibodies in serum, against Schistosoma spp. in endemic and non-endemic populations. Methodology/Principal findings Frozen banked serum samples from patients with confirmed schistosomiasis, patients with other helminth infections, patients with seropositive rheumatoid arthritis and healthy blood donors were used to assess the sensitivity and the specificity of the Schistosoma ICT IgG-IgM rapid diagnostic test. The test showed a sensitivity of 100% in patients with parasitologically confirmed schistosomiasis, irrespective of the species (S. mansoni, S. haematobium, S. japonicum, S. mekongi). In healthy blood donors and patients with rheumatoid factor positive rheumatoid arthritis from Europe, specificity was 100%. However, in serum samples of patients with other tissue invasive helminth infections, the test showed some cross-reactivity, resulting in a specificity of 85%. Conclusion/Significance With its high sensitivity, the Schistosoma ICT IgG-IgM rapid diagnostic test is a suitable screening test for detection of Schistosoma specific antibodies, including S. mekongi. However, in populations with a high prevalence of co-infection with other tissue invasive helminths, positive results should be confirmed with other diagnostic assays due to the test’s imperfect specificity.

show abstract

Parasitological and immunological aspects of oral and subcutaneous prednisolone treatment in rats experimentally infected with Strongyloides venezuelensis

et al. 2020

View full text Add to dashboard Cite

Serological cross-reactivity between Strongyloides venezuelensis and Syphacia muris in Wistar rats (Rattus norvegicus)

Cited by 6 publications

References 31 publications

Immunoreactivity of proteins within 30-40 kDa range during the acute and the recovery phases in rats experimentally infected with Strongyloides venezuelensis

Immunoreactivity of proteins within 30-40 kDa range during the acute and the recovery phases in rats experimentally infected with Strongyloides venezuelensis

Performance of a rapid immuno-chromatographic test (Schistosoma ICT IgG-IgM) for detecting Schistosoma-specific antibodies in sera of endemic and non-endemic populations

Parasitological and immunological aspects of oral and subcutaneous prednisolone treatment in rats experimentally infected with Strongyloides venezuelensis

Contact Info

Product

Resources

About