Parasitological and immunological aspects of oral and subcutaneous prednisolone treatment in rats experimentally infected with Strongyloides venezuelensis

Couto, Bruna Patricia do; Corrêa, Luisa Queiroz; Sousa, José Eduardo Neto de; Carvalho, Edson Fernando Goulart de; Gonzaga, Henrique Tomaz; Costa-Cruz, Júlia Maria

doi:10.1016/j.actatropica.2020.105349

Cited by 2 publications

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“…Antibody levels: indirect ELISA to detect total IgG, IgG1 and IgG2a levels Saline extract used was produced from 300,000 larioid larvae (Gonzaga et al 2011). The follow up of speci c S. venezuelensis IgGantibodies and IgG1 and IgG2a detection were done by indirect ELISA, according to (Couto et al 2020). ELISA protocols were performed using pooled serum samples from each group for each experimental day tested.…”

Section: Parasite Burden: Eggs Per Gram Of Feces and Parasitic Female...mentioning

confidence: 99%

“…A signi cant difference between S. stercoralis, the human pathogen, and S. venezuelensis, the rodent one, life cycles is that hyperinfection and extremely chronic infections are present only in S. stercoralis infection (Breloer and Abraham 2017). Studies have used murine infected with S. venezuelensis and pharmacologically immunosuppressed by glucocorticoid treatments as an experimental model of chronic and complicated forms of strongyloidiasis (Carvalho et al 2015;Chaves et al 2015;Couto et al 2020).…”

Section: Introductionmentioning

confidence: 99%

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Route of dexamethasone administration influences parasite burden in Strongyloides hyperinfection model

et al. 2023

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Rodents infected with Strongyloides venezuelensis are experimental models applied to strongyloidiasis research. This study evaluated oral and subcutaneous dexamethasone (DEX) treatments to establish immunosuppression in an experimental model of Strongyloides hyperinfection. Rattus norvegicus Wistar were divided: G I (−): untreated and uninfected animals, G II (+): untreated and infected, G III (o −) orally treated and uninfected, G IV (o +) orally treated and infected, G V (sc −) subcutaneously treated and uninfected, G VI (sc +) subcutaneously treated and infected. For oral administration, DEX was diluted in sterile water (5 µg / ml) and made available to the animals on intervals in experimental days − 5 to 0, 8 to 13 and 21 to 26. For subcutaneous administration, animals received daily injections of DEX disodium phosphate (2mg / kg). Infection was established by the subcutaneous inoculation of 3000 S. venezuelensis larioid larvae. Groups were evaluated by egg per gram of feces and parasite females counts and IgG, IgG1 and IgG2a detection. GIV (o+) had egg peaks count on days 13 and 26 and maintained egg elimination until the last experimental day. Parasitic females recovery at day 30 was signi cantly higher in G IV (o+) when compared to G VI (sc +). Levels of IgG, IgG1 and IgG2a of all groups, except the positive control GII (+), were below the detection threshold. Pharmacological immunosuppression induced by oral administration of DEX produced high parasitic burden, and is a noninvasive method, useful to study immunosuppression in strongyloidiasis hyperinfection model in rats.

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Section: Parasite Burden: Eggs Per Gram Of Feces and Parasitic Female...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Route of dexamethasone administration influences parasite burden in Strongyloides hyperinfection model

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

Route of dexamethasone administration influences parasite burden in Strongyloides hyperinfection model

Corrêa

Couto

Carvalho

et al. 2022

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Rodents infected with Strongyloides venezuelensis are experimental models applied to strongyloidiasis research. This study evaluated oral and subcutaneous dexamethasone (DEX) treatments to establish immunosuppression in an experimental model of Strongyloides hyperinfection. Rattus norvegicus Wistar were divided: G I (−): untreated and uninfected animals, G II (+): untreated and infected, G III (o −) orally treated and uninfected, G IV (o +) orally treated and infected, G V (sc −) subcutaneously treated and uninfected, G VI (sc +) subcutaneously treated and infected. For oral administration, DEX was diluted in sterile water (5 µg / ml) and made available to the animals on intervals in experimental days − 5 to 0, 8 to 13 and 21 to 26. For subcutaneous administration, animals received daily injections of DEX disodium phosphate (2mg / kg). Infection was established by the subcutaneous inoculation of 3000 S. venezuelensis filarioid larvae. Groups were evaluated by egg per gram of feces and parasite females counts and IgG, IgG1 and IgG2a detection. GIV (o+) had egg peaks count on days 13 and 26 and maintained egg elimination until the last experimental day. Parasitic females recovery at day 30 was significantly higher in G IV (o+) when compared to G VI (sc +). Levels of IgG, IgG1 and IgG2a of all groups, except the positive control GII (+), were below the detection threshold. Pharmacological immunosuppression induced by oral administration of DEX produced high parasitic burden, and is a noninvasive method, useful to study immunosuppression in strongyloidiasis hyperinfection model in rats.

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Parasitological and immunological aspects of oral and subcutaneous prednisolone treatment in rats experimentally infected with Strongyloides venezuelensis

Cited by 2 publications

References 30 publications

Route of dexamethasone administration influences parasite burden in Strongyloides hyperinfection model

Route of dexamethasone administration influences parasite burden in Strongyloides hyperinfection model

Route of dexamethasone administration influences parasite burden in Strongyloides hyperinfection model

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