Serological and molecular detection of Mycobacterium avium subsp. paratuberculosis in cattle of dairy herds in Colombia

Abstract

The objective of this study is the detection of Mycobacterium avium subsp. paratuberculosis (MAP) by serum enzyme-linked immunosorbent assay (ELISA), fecal polymerase chain reaction (PCR), and fecal culture in Colombian dairy herds. Serum and fecal samples from asymptomatic cows (n = 307) of 14 dairy herds were tested for MAP by an unabsorbed ELISA test (ELISA-A). Serum and fecal samples from positive ELISA-A animals (n = 31) were further tested by an absorbed ELISA test (ELISA-B) and PCR. Fecal samples from animals of herds positive by ELISA-A and PCR (n = 105) were inoculated onto three different culture media. ELISA-A produced positive results in 10% of the serum samples and 71% of the herds. ELISA-B and PCR results were positive in two and six serum and fecal samples from positive ELISA-A animals, respectively. Fecal samples were negative for MAP on all culture media. The results of this study confirmed the presence of MAP in local dairy herds and the difficulties of MAP detection in asymptomatic animals by ELISA, PCR, and fecal culture.

Diagnóstico clínico e histopatológico de paratuberculosis bovina en un hato lechero en Colombia

“…Los casos compatibles con paratuberculosis se presentaron siempre de manera individual y esporádica a lo largo del tiempo. Varios estudios diagnósticos realizados a diferentes animales en ese hato entre 2000 y 2010, han proporcionado resultados que indican la presencia de MAP en el rebaño (16)(17)(18). A pesar del seguimiento de los casos compatibles y los exámenes realizados a los animales del hato, los resultados de diagnóstico clínico e histopatológico obtenidos durante el periodo de tiempo 2000-2008 no se habían analizado sistemáticamente hasta la fecha.…”

Serological and molecular diagnosis of paratuberculosis in dairy cattle

“…Positivity was detected in 89 (4.6%) of 1950 serum samples (6) and in 424 (12.4%) of 10,280 samples in cattle (21), while 3.48% of 3961 cattle over 2 years of age were found to be positive in terms of seroprevalence in Poland (22). In Colombia, Fernandez-Silva et al (23) determined positivity at a rate of 10.1%, while in Turkey, Makav and Gokce (24) found a 3.5% prevalence rate for subclinical paratuberculosis in the Kars region, and in Burdur Province a rate of 6.2% was reported by Ozturk et al (25). In the present study, in Kayseri Province, 12.24% and 10.88% of 147 blood samples taken from paratuberculosis-suspected animals were detected as positive and suspicious by ELISA, respectively.…”

Mycobacterium avium subsp. paratuberculosis in Colombia (1924-2016): A review

“…It is necessary to define the exact status of the disease through well-designed prevalence/incidence studies, considering that no whole national data is available. In this regard, just some local estimates are currently available (Patiño and Estrada, 1999;Ramírez et al, 2001;Fernández et al, 2011a;2011b;Benavides et al, 2016;Correa et al, 2016).…”

“…Only one previous study reported molecular characterization of strains isolated in Colombia (Fernández et al, 2011b), being this insufficient to consider the definition of "indigenous strains" and the ulterior design of vaccines. It would be necessary to conduct studies on wider regions, considering infectionassessment on cattle and other-than-cattle susceptible populations (even local wildlife) to generate prophylactic strategies according to Colombian MAP molecular and epidemiological diversity.…”