Serological and coprological comparison for rapid diagnosis of Fasciola hepatica infection in small ruminants from sub-tropical area of Pakistan

Afshan, Kiran; Qayyum, Mazhar; Rizvi, Syed Shakeel Raza; Mukhtar, Muhammad Mahmood; Mushtaq, Maria; Miller, James E.

doi:10.1016/j.smallrumres.2013.01.020

Cited by 15 publications

(20 citation statements)

References 24 publications

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“…IgG antibody. These findings may reflect a low immune response to the antigenic stimulus from the migrating or mature flukes in the cattle which result in undetectable antibody ( Afshan et al . 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Risk Factors of Bovine Fascioliasis in Kelantan, Malaysia: A Cross-Sectional Study

Ahmad-Najib

Wan-Nor-Amilah

Kin

et al. 2021

TLSR

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Bovine fascioliasis is an important zoonotic parasitic disease that causes significant economic losses to the livestock industry. The aim of this study was to determine the prevalence and risk factors of bovine fascioliasis in Kelantan. In this cross-sectional study, a total of 308 stool and blood samples of farmed cattle were collected from December 2017 to June 2018. The stool samples were examined microscopically for the presence of Fasciola spp. eggs following a formalin-ether sedimentation process. The blood samples were subjected to a commercial ELISA kit (Bio-X-Diagnostic, Rochefort, Belgium) for the detection of anti-Fasciola IgG antibody. The association between coprological findings and risk factors was determined using Pearson’s chi-square (χ2). The coproprevalence and seroprevalence of bovine fascioliasis was 14.6% and 37.3%, respectively. There were significant (P < 0.05) associations between the risk of infections and the sex, type of feedings, anthelmintic treatment and farm hygiene. Female cattle (OR: 3.104; 95% CI: 1.265, 7.615), feeding by grazing (OR: 4.458; 95% CI: 1.823, 10.90), untreated cattle (OR: 3.833; 95% CI: 1.620, 9.071), non-schedule anthelminthic treatment (OR: 3.927; 95% CI: 1.685, 9.152) and farm that have never been cleaned (OR: 2.829; 95% CI: 1.428, 5.608) showed higher odds of Fasciola spp. infection. These findings suggested bovine fascioliasis is a serious veterinary disease in Kelantan. Thus, appropriate control, prevention and monitoring strategies of this parasitic infection are urgently needed to reduce the burden of the disease.

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Section: Discussionmentioning

confidence: 99%

Prevalence and Risk Factors of Bovine Fascioliasis in Kelantan, Malaysia: A Cross-Sectional Study

Ahmad-Najib

Wan-Nor-Amilah

Kin

et al. 2021

TLSR

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“…However, the costs for performing the coproantigen ELISA are much higher than those for the coproscopic method, which needs only basic laboratory equipment and tap water. The detection of specific anti- F. hepatica -antibodies in serum samples with an ELISA technique is a suitable method for early diagnosis of infection [ 34 ]. In experimentally infected sheep, specific antibodies were detectable within two weeks p.i.…”

Section: Fasciolidae With Emphasis On the Common Liver Flukementioning

confidence: 99%

Chronic Wasting Due to Liver and Rumen Flukes in Sheep

Kahl

Samson‐Himmelstjerna

Krücken

et al. 2021

Animals

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Grazing sheep and goats are constantly exposed to helminth infections in many parts of the world, including several trematode species that causes a range of clinical diseases. The clinical picture of flukes is dependent upon the organs in which they develop and the tissues they damage within the respective organs. Accordingly, infections with the common liver fluke Fasciola hepatica, which, as juvenile worm migrates through the liver parenchyma for several weeks, may be associated with hepatic disorders such as impairment of carbohydrate, protein and fat metabolism, followed by chronic wasting. In contrast, the lancet fluke Dicrocoelium dendriticum, which does not exhibit tissue migration and thus does not lead to major tissue damage and bleeding, also does not lead to significant clinical symptoms. Rumen flukes such as Cotylophoron daubneyi cause catarrhal inflammation during their migration through the intestinal and abomasal epithelium during its juvenile stages. Depending on the infection intensity this may result in a range of clinical symptoms including diarrhoea, inappetence or emaciation. In this review, we aim to provide an update on the current knowledge on flukes particularly concerning the clinical relevance of the most important fluke species in sheep.

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“…The prevalence of infection was recorded to be higher for coproantigen ELISA (77.20%) than indirect ELISA serological test (73.46%). Additionally, 3.74% coproantigen-positive goats were found to be negative to serum IgG1 ELISA, which may indicate that the goat's immune system did not respond adequately to the antigenic stimulus from migrating or mature flukes [ 8 ]. Prevalence of 43.0% was detected using indirect ELISA in goats from a semidesert area in the northwest of Mexico [ 10 ].…”

Section: Discussionmentioning

confidence: 99%

“…In Galega goats from northwestern Spain, seroprevalence of 22.7% was found using capture ELISA (MM3 antigen comprised cathepsins L1 and L2 and a Kunitz-like protein) [ 32 ]. The prevalence of fasciolosis in Pakistan has been reported using a commercially available indirect ELISA kit (DRG, Germany) in goats and ranged between 4.08% and 49.36% [ 8 , 33 ]. Over the past decade, the prevalence of F. hepatica infection has risen in part due to climate change, increased animal movement, and changing farming practices [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

“…Although caprine infection is lower than ovine or bovine fasciolosis, goats are extremely sensitive and susceptible to both natural and experimental infections [ 6 , 7 ]. F. hepatica has been also detected in temperate cooler areas of high lands of tropical and subtropical regions [ 8 ]. Mexico possesses 8.6 million goats [ 9 ] located in areas with lowest human development index, with high potential to improve economy.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Diagnosis of Serum IgG1 and Coproantigen ELISA for Fasciolosis Detection of Goats in Mexico

Villa-Mancera

Molina-Mendoza

Hernández-Guzmán

et al. 2016

BioMed Research International

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The objective of present study was to determine the prevalence of natural caprine fasciolosis in the Mixteca region of Mexico using coproantigen and serum IgG1 ELISA tests for comparative purposes. A total of 1070 serum and faecal samples were analyzed for IgG1 antibodies and coproantigens, using ELISA with E/S products as antigen and a monoclonal antibody-based sandwich ELISA. Prevalence of 73.46% was found using the serological ELISA and a percentage of 77.20 was found for coproantigen ELISA. The diagnostic sensitivity and specificity for serum ELISA were 86.7% and 96.4%, and for the coproantigen ELISA they were 93.1% and 97.8%, respectively. The seropositive samples were further categorized as low, medium, or high positivity. Results show a great proportion of low and medium positive goats when the serum ELISA test was used. Correlation coefficients between coproantigens and seropositivity were statistically significant (P < 0.01) for low seropositivity (r = 0.93) and medium seropositivity (r = 0.84). The accuracy of faecal antigen ELISA was higher compared to indirect ELISA serological test. Two ELISAs were shown to be useful for demonstrating the current status of F. hepatica infection in the endemic areas and can be employed in studies on epidemiology as well as anthelmintics treatment for preventing economic loss and the risk of transmission to humans.

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Serological and coprological comparison for rapid diagnosis of Fasciola hepatica infection in small ruminants from sub-tropical area of Pakistan

Cited by 15 publications

References 24 publications

Prevalence and Risk Factors of Bovine Fascioliasis in Kelantan, Malaysia: A Cross-Sectional Study

Prevalence and Risk Factors of Bovine Fascioliasis in Kelantan, Malaysia: A Cross-Sectional Study

Chronic Wasting Due to Liver and Rumen Flukes in Sheep

Comparative Diagnosis of Serum IgG1 and Coproantigen ELISA for Fasciolosis Detection of Goats in Mexico

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