Seroimmunological affiliation of African swine fever virus isolated in the Russian Federation

Балышев, В. М.; Kalantaenko, Yu. F.; Bolgova, M. V.; Prodnikova, E. Yu.

doi:10.3103/s1068367411050053

Cited by 11 publications

(20 citation statements)

References 3 publications

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“…Eight ASFV serogroups have been identified, although more likely exist (Balyshev et al, 1995(Balyshev et al, , 2010Sereda & Balyshev, 2011;Sereda et al, 1992;Vishnjakov et al, 1995). Notably and of particular significance for vaccine development, ASF protective immunity appears to be serotype-specific, as viruses within a serogroup cross-protect against one another (Balyshev et al, 1995;Sereda & Balyshev, 2011;Sereda et al, 1992;Vishnjakov et al, 1991;A.…”

mentioning

confidence: 99%

African swine fever virus CD2v and C-type lectin gene loci mediate serological specificity

Malogolovkin

Burmakina

Tulman

et al. 2015

Journal of General Virology

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African swine fever (ASF) is an emerging disease threat for the swine industry worldwide. No ASF vaccine is available and progress is hindered by lack of knowledge concerning the extent of ASF virus (ASFV) strain diversity and the viral antigens responsible for protection in the pig. Available data from vaccination/challenge experiments in pigs indicate ASF protective immunity is haemadsorption inhibition (HAI) serotype-specific. A better understanding of ASFV HAI serological groups and their diversity in nature, as well as improved methods to serotype ASFV isolates, is needed. Here, we demonstrated that the genetic locus encoding ASFV CD2v and Ctype lectin proteins mediates HAI serological specificity and that CD2v/C-type lectin genotyping provides a simple method to group ASFVs by serotype, thus facilitating study of ASFV strain diversity in nature, and providing information necessary for eventual vaccine design, development and efficacious use.

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confidence: 99%

African swine fever virus CD2v and C-type lectin gene loci mediate serological specificity

Malogolovkin

Burmakina

Tulman

et al. 2015

Journal of General Virology

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“…Nine distinct seroimmunological groups (seroimmunotypes) have been identified to date (the reference strains are shown in parentheses): I (Lisbon‐57), II (Congo‐49), III (Mozambique‐78), IV (France‐32), V (TSP‐80), VI (TS‐7), VII (Uganda), VIII (Rhodesia and Stavropol 2008), and IX (Davis). All Russian ASFVs isolated since 2007 were identified as the seroimmunotype VIII, whereas the European viruses represent the seroimmunotype I or IV (Balyshev et al., , , , ; Sereda and Balyshev, ; Vlasova et al., ). As the current genotype classification is irrelevant to antigenic and immunogenic properties of ASFV (Malogolovkin et al., ), the seroimmunological characteristics of ASFV isolates should be taken into consideration in the development of the systems for serological diagnosis of ASF (Balyshev et al., , ; Sereda and Balyshev, ).…”

Section: Introductionmentioning

confidence: 99%

“…All Russian ASFVs isolated since 2007 were identified as the seroimmunotype VIII, whereas the European viruses represent the seroimmunotype I or IV (Balyshev et al., , , , ; Sereda and Balyshev, ; Vlasova et al., ). As the current genotype classification is irrelevant to antigenic and immunogenic properties of ASFV (Malogolovkin et al., ), the seroimmunological characteristics of ASFV isolates should be taken into consideration in the development of the systems for serological diagnosis of ASF (Balyshev et al., , ; Sereda and Balyshev, ). The isolate Stavropol 2008 was one of the first ASFV isolated from the domestic pigs in the Russian Federation.…”

Section: Introductionmentioning

confidence: 99%

Recombinant Protein p30 for Serological Diagnosis of African Swine Fever by Immunoblotting Assay

Kazakova

Imatdinov

Dubrovskaya

et al. 2016

Transbound Emerg Dis

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This article is devoted to the development and evaluation of the immunoblotting test system for serological diagnosis of African swine fever (ASF), based on the highly purified recombinant p30 of ASF virus (ASFV) strain Stavropol 01/08 (Stavropol 2008), representative of the ASFV currently circulating in the Russian Federation. The main project stages are as follows: (i) cloning of the central hydrophilic region of the ASFV gene CP204L (p30) into a prokaryotic vector; (ii) expression and chromatographic purification of the recombinant product p30 with thioredoxin and poly-histidine site (p30e1_TrxA_6xHis); (iii) development of the immunoblotting test system (Rec p30-IB) using the highly purified recombinant p30; and (iv) evaluation of Rec p30-IB using sera and organ samples from domestic pigs and wild boars experimentally or naturally infected by ASFV. Testing of the Rec p30-IB showed the diagnostic specificity and sensitivity of the assay to be 98.75% and 100.00%, respectively. High sensitivity of the Rec p30-IB allowed the detection of ASFV-specific antibodies in samples of organs of the immune system and blood sera, collected from domestic pigs and wild boars, starting from 6 to 8 days post-infection, regardless of virus virulence, seroimmunotype and geographic origin of the samples (East Europe, South Europe, West Europe, Central and south-east Africa).

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“…The effective development of intensive pig farming is hindered to a large extent by different infectious diseases which cause a great economic damage characterised with significant mortality and animal productive capacity losses (Sobko et al, 1988;Balyshev et al, 2010). At present, African swine fever (Pestis Africana suum, ASF) constitutes a threat of highest priority for global pig industry (Makarov et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

African swine fever in the Krasnodar region

Prutsakov¹,

Kruzhnov²,

Bolotsky³

et al. 2015

BJVM

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Monitoring of the epidemic situation with African swine fever was carried out in the Krasnodar region (federal subject of Russia) in 2008-2013. This made it possible to determine sources, routes and factors promoting African swine fever spread. The major sources of the epidemic were presented by sick and dead wild and domestic pigs, non-decontaminated condemned meat products. During this time forty three outbreaks of this disease were reported in this region; more than 280 ths of pigs died and were destroyed, 36 pig enterprises were closed and it caused a great economic damage to pig farming industry of the region. Transmission factors were as followed: transport facilities that visited affected focal points and were not decontaminated to an accomplished standard, contaminated by African swine fever virus feeds, equipment, etc. Predictions on a possibility of occurrence of new disease cases in time and space are made using available information on ASF spread in the territory of the RF. As a result, a possibility to coordinate future efforts aimed at the disease surveillance in corresponding territories independently of reports from the given region emerges.

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Seroimmunological affiliation of African swine fever virus isolated in the Russian Federation

Cited by 11 publications

References 3 publications

African swine fever virus CD2v and C-type lectin gene loci mediate serological specificity

African swine fever virus CD2v and C-type lectin gene loci mediate serological specificity

Recombinant Protein p30 for Serological Diagnosis of African Swine Fever by Immunoblotting Assay

African swine fever in the Krasnodar region

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