2018
DOI: 10.1590/1678-4162-9369
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Abstract: This study aimed to identify serogroups of Escherichia coli important for human health in isolates from psittacine of illegal wildlife trade in Ceará State. In addition, hemolysis and production of Extended Spectrum Beta-Lactamases (ESBL) was assessed in the isolates. A total of 78 E. coli strains isolated from different Psittaciformes species from a wildlife rehabilitation center in Fortaleza, Brazil. The isolates used in this study were previously identified and stored. Serogroup identification was performed… Show more

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“…Hemolysin production was demonstrated as described by Edberg et al [ 45 ]. Overnight single colony was sub-cultured on 5% defibrinated sheep blood agar overlaid on the Nutrient agar base (Oxoid, UK) and incubated at 37 °C for 72 h. A clear halo zone indicating lysis of the red blood cells around the colony indicates haemolysin production [ 46 ]. A phenotypic assay for protease production was performed according to Suganthi et al [ 47 ].…”
Section: Methodsmentioning
confidence: 99%
“…Hemolysin production was demonstrated as described by Edberg et al [ 45 ]. Overnight single colony was sub-cultured on 5% defibrinated sheep blood agar overlaid on the Nutrient agar base (Oxoid, UK) and incubated at 37 °C for 72 h. A clear halo zone indicating lysis of the red blood cells around the colony indicates haemolysin production [ 46 ]. A phenotypic assay for protease production was performed according to Suganthi et al [ 47 ].…”
Section: Methodsmentioning
confidence: 99%