Seroepidemiology of Spotted Fever Group Rickettsiae in Small Field Rodents in Japan

Ishikura, Mitsuhiro; Watanabe, Mamoru; Nakayama, Takashi; Matsuura, Kumiko; Morita, Osayuki; Uchida, Takahiro

doi:10.1111/j.1348-0421.1992.tb02066.x

Cited by 6 publications

(5 citation statements)

References 17 publications

(19 reference statements)

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“…The detection rate of the rickettsial gltA genes from ticks was an average of 26%. We already reported that many field rodents in Japan possess anti-SFG rickettsia antibody (13). In the present study, we clarified that an average of 26% of ticks in Japan carry the rickettsiae, and most rickettsiae in those ticks were SFG rickettsiae.…”

Section: Discussionsupporting

confidence: 58%

Phylogenetic Analysis of Spotted Fever Group Rickettsiae Based on gltA, 17‐kDa, and rOmpA Genes Amplified by Nested PCR from Ticks in Japan

Ishikura

Ando

Shinagawa

et al. 2003

Microbiology and Immunology

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118

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Rickettsiae belongs to the order Rickettsiales, whose members were described as obligate intracellular gramnegative microorganisms. Several species cause diseases in humans and other vertebrate or invertebrate hosts, and have a worldwide distribution. For the last 15 years, nine new rickettsiosis have been reported, that is, Japanese spotted fever caused by Rickettsia japonica (19,39), Flinders Island spotted fever caused by R. honei (33,34), Astrakhan tick bite fever caused by Astrakhan fever rickettsia (5), African tick bite fever caused by R. africae (15), California flea typhus caused by R. felis (the ELB agent) (35), and four other unnamed spotted fevers caused by R. mongolotimonae (30,44), R. slovaca (18, 31, 36), R. helvetica (11, 25), and "R. heilongjiangii," (17, 45) respectively. In Japan, 63 tick-associated rickettsiae strains, belonging to at least three serotypes, have been isolated over the last several years (8-10), including R. japonica isolated from the Dermacentor taiwanensis and Haemaphysalis frava ticks, respectively; R. helvetica (strains IP-1 and IM-1), strains Abstract: In order to understand the natural situation of rickettsiae in the ticks in Japan, the rickettsial genes, gltA gene, rOmpA gene, and 17-kDa gene, were amplified from the ticks by nested PCR. The prevalences of rickettsial gltA genes among Haemaphysalis formosensis, H. longicornis, H. megaspinosa, Ixodes ovatus, H. flava, H. kitaokai, and I. persulcatus were 62, 57, 24, 24, 19, 13, and 10%, respectively; 26% (186/722) being the average. The gltA genes amplified from the ticks were classified into 9 genotypes (I to IX) by the difference in nucleotide sequences. Genotype I was detected from 7 species of ticks. Genotype II mainly was detected from H. longicornis and H. formosensis. Genotypes III and VII mainly were detected from H. flava and I. ovatus. The polarization in the distribution of genotypes among regions where the ticks were collected was not clear. Based on the phylogenetic analysis of the three genes presented here, genotypes I, III, and IV (detected from H. formosensis, H. hystricia, and I. ovatus) are genetically close with each other, but rickettsiae of the same property still have not been isolated from ticks anywhere in the world. These genotypes should be considered as new species among SFG rickettsiae. Genotype II was identical with strain FUJ-98, genetically close to R. japonica which has been isolated from ticks in China. Genotype V was identical with R. felis and strain California 2 isolated from the cat flea. This is the first report on the detection of R. felis from ticks. Genotype VI detected from Ixodes sp. did not seem to belong to genus Rickettsia. Based on the previous antigenic data and the phylogenetic analysis presented here, Genotype VII should be considered a variant of R. helvetica and genotype VIII detected from I. ovatus and I. persulcatus were identical with R. helvetica. Genotype IX detected from I. nipponensis was genetically close to the strains IRS3, IRS4, and IrR/Munich isolated from...

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Section: Discussionsupporting

confidence: 58%

Phylogenetic Analysis of Spotted Fever Group Rickettsiae Based on gltA, 17‐kDa, and rOmpA Genes Amplified by Nested PCR from Ticks in Japan

Ishikura

Ando

Shinagawa

et al. 2003

Microbiology and Immunology

Self Cite

118

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“…8 Laboratory studies have demonstrated that some small rodents species are competent hosts of rickettsiae and develop rickettsemia levels capable of infecting their ectoparasites, despite the short period of rickettsemia. 3,9 There have been reports showing R. rattus to contain antibodies against rickettsial antigens [8][9][10] Furthermore, our study is the this first to identify a prevalent immune response in the other mammalian species by IFA using specific rickettsial antigens. Further studies are necessary to better understand the actual importance of these animals in the sylvan and domestic cycles of BSF, as well their interaction with their arthropods vectors.…”

Section: Discussionmentioning

confidence: 92%

Spotted Fever Group Rickettsia in Small Rodents from Areas of Low Endemicity for Brazilian Spotted Fever in the Eastern Region of Minas Gerais State, Brazil

Milagres¹,

Padilha²,

Montandon³

et al. 2013

The American Society of Tropical Medicine and Hygiene

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Abstract. We investigated the humoral immune response against different species of Rickettsia in serum samples from small rodents collected in two areas of a silent focus for Brazilian spotted fever in the eastern region of Minas Gerais State, Brazil. Sera samples were analyzed by indirect immunofluorescence assay using antigens from Rickettsia species of the spotted fever, ancestral, and transition groups. Titers 1:64 were considered positive. In Santa Cruz do Escalvado, 94% (30 of 32) of the samples collected from Rattus rattus, 22% (5 of 23) from Nectomys squamipes, and 80% (4 of 5) from Akodon sp., reacted by indirect immunofluorescence assay with Rickettsia antigens of the spotted fever group. In the municipality of Pingo D'Á gua, 84% (26 of 31) of the samples collected from R. rattus, 86% (6 of 7) of the samples from Oryzomys subflavus, 86% (6 of 7) from N. squamipes, and 100% (1 of 1) from Bolomys sp. contained antibodies that reacted with rickettsial antigens of the spotted fever group. These results demonstrated the previous exposure of small rodents to spotted fever group Rickettsia, suggesting the participation of these animals in the natural history of these rickettsiae in this region.

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“…These findings suggest that SFG rickettsia is distributed in Okinawa and that dogs are also an important mammalian reservoir of SFG rickettsia in addition to the usual wild rodents (7,9,26). However, the SFG rickettsia in Okinawa has not been genetically characterized.…”

Section: Discussionmentioning

confidence: 78%

Characterization of Spotted Fever Group Rickettsiae Detected in Dogs and Ticks in Okinawa, Japan

Satoh

Motoi

Camer

et al. 2002

Microbiology and Immunology

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Spotted fever group (SFG) rickettsial DNAs were detected in 2.4% of 340 canine blood samples and a pool of 84 tick pool samples (229 ticks) collected in Okinawa, Japan by PCR using a citrate synthase and an SFG rickettsial 190‐kDa surface antigen gene primer pair. The sequences of both genes from canine blood and tick samples showed high levels of similarity with those of Rickettsia japonica and several SFG rickettsiae (R. aeschlimannii, R. massiliae, R. rhipicephali and Bar‐29 strain). Phylogenesis of canine blood and tick samples was closely related to that of reference SFG rickettsiae. Serological evidence of SFG rickettsial infection in dogs and humans in Okinawa, where no clinical human cases have been reported, has been obtained. In this study, genetical characterization of SFG rickettsia in Okinawa was investigated phylogenetically.

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Seroepidemiology of Spotted Fever Group Rickettsiae in Small Field Rodents in Japan

Cited by 6 publications

References 17 publications

Phylogenetic Analysis of Spotted Fever Group Rickettsiae Based on gltA, 17‐kDa, and rOmpA Genes Amplified by Nested PCR from Ticks in Japan

Phylogenetic Analysis of Spotted Fever Group Rickettsiae Based on gltA, 17‐kDa, and rOmpA Genes Amplified by Nested PCR from Ticks in Japan

Spotted Fever Group Rickettsia in Small Rodents from Areas of Low Endemicity for Brazilian Spotted Fever in the Eastern Region of Minas Gerais State, Brazil

Characterization of Spotted Fever Group Rickettsiae Detected in Dogs and Ticks in Okinawa, Japan

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