Seroepidemiology of human bocavirus in Apulia, Italy

Guido, Marcello; Zizza, Antonella; Bredl, Simon; Lindner, Juha; Donno, Antonella De; Quattrocchi, M.; Grima, Pierfrancesco; Modrow, Susanne

doi:10.1111/j.1469-0691.2011.03756.x

Cited by 22 publications

(22 citation statements)

References 20 publications

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“…Our results was in agreement with Jiang et al, (2016) who reported that the infections almost completely confine to infants and young children also in agreement with most of the previous studies by Allender et al, (2005), Choi et al, (2006), Chow et al, (2009) and Abdel-Moneim et al, (2013). However, some authors reported that HBoV is also a frequent virus in adults with respiratory disease Guido et al, (2012) and Ghietto et al, (2012).…”

Section: Resultssupporting

confidence: 94%

Detection of Human Bocavirus in Egyptian Children Suffering from Acute Lower Respiratory Tract Infection

Atef¹,

Elbehedy²

2017

Int.J.Curr.Microbiol.App.Sci

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Section: Resultssupporting

confidence: 94%

Detection of Human Bocavirus in Egyptian Children Suffering from Acute Lower Respiratory Tract Infection

Atef¹,

Elbehedy²

2017

Int.J.Curr.Microbiol.App.Sci

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“…A serological survey performed in Italy to determine the prevalence of antibodies against human bocavirus in an Apulian population, indicate HBoV infection is ubiquitous in the general population and early acquired from preschool children (Guido et al 2012), with more than 90 % of subjects older than 4 years displaying previous exposure to HBoV. Our results support the notion that HBoV is widely circulating in the population with prevalence of human bocavirus 2 and 3, and a large number of samples containing both these species.…”

Section: Discussionsupporting

confidence: 80%

Frequent Detection and Genetic Diversity of Human Bocavirus in Urban Sewage Samples

et al. 2016

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The prevalence and genetic diversity of human bocaviruses (HBoVs) in sewage water samples are largely unknown. In this study, 134 raw sewage samples from 25 wastewater treatment plants (WTPs) in Italy were analyzed by nested PCR and sequencing using species-specific primer pairs and broad-range primer pairs targeting the capsid proteins VP1/VP2. A large number of samples (106, 79.1 %) were positive for HBoV. Out of these, 49 were classified as HBoV species 2, and 27 as species 3. For the remaining 30 samples, sequencing results showed mixed electropherograms. By cloning PCR amplicons and sequencing, we confirmed the copresence of species 2 and 3 in 29 samples and species 2 and 4 in only one sample. A real-time PCR assay was also performed, using a newly designed TaqMan assay, for quantification of HBoVs in sewage water samples. Viral load quantification ranged from 5.51E+03 to 1.84E+05 GC/L (mean value 4.70E+04 GC/L) for bocavirus 2 and from 1.89E+03 to 1.02E+05 GC/L (mean value 2.27E+04 GC/L) for bocavirus 3. The wide distribution of HBoV in sewages suggests that this virus is common in the population, and the most prevalent are the species 2 and 3. HBoV-4 was also found, representing the first detection of this species in Italy. Although there is no indication of waterborne transmission for HBoV, the significant presence in sewage waters suggests that HBoV may spread to other water environments, and therefore, a potential role of water in the HBoV transmission should not be neglected.

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“…Thus, acute infections can be diagnosed by antibody assays; detection of IgM, seroconversion, or an increase in IgG titer in paired serum samples and low IgG avidity are markers of an acute primary infection (8,(40)(41)(42). In addition, epidemiological information can be gathered by measuring the IgG seroprevalence in the population (9,(43)(44)(45)(46). These studies report seroprevalences of up to 100% in adults.…”

mentioning

confidence: 99%

Mapping Antigenic Epitopes on the Human Bocavirus Capsid

Kailasan

Garrison

Ilyas

et al. 2016

J Virol

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Human bocaviruses (HBoV1 to -4) are emerging pathogens associated with pneumonia and/or diarrhea in young children. Currently, there is no treatment or vaccination, so there is a need to study these pathogens to understand their disease mechanisms on a molecular and structural level for the development of control strategies. Here, we report the structures of six HBoV monoclonal antibody (MAb) fragment complexes, HBoV1-15C6, HBoV2-15C6, HBoV4-15C6, HBoV1-4C2, HBoV1-9G12, and HBoV1-12C1, determined by cryo-electron microscopy and three-dimensional image reconstruction to 18.0-to 8.5-Å resolution. Of these, the 15C6 MAb cross-reacted with HBoV1, HBoV2, and HBoV4, while the 4C2, 12C1, and 9G12 MAbs recognized only HBoV1. Pseudoatomic modeling mapped the 15C6 footprint to the capsid surface DE and HI loops, at the 5-fold axis and the depression surrounding it, respectively, which are conserved motifs in Parvoviridae. The footprints for 4C2, 12C1, and 9G12 span the surface loops that assemble portions of the 2-/5-fold wall (a raised surface feature between the 2-fold and 5-fold axes of symmetry) and the shoulder of the 3-fold protrusions. The MAb footprints, cross reactive and strain specific, coincide with regions with high and low sequence/structural identities, respectively, on the capsid surfaces of the HBoVs and identify potential regions for the development of peptide vaccines for these viruses. IMPORTANCEHuman bocaviruses (HBoVs) may cause severe respiratory and gastrointestinal infections in young children. The nonenveloped parvovirus capsid carries determinants of host and tissue tropism, pathogenicity, genome packaging, assembly, and antigenicity important for virus infection. This information is currently unavailable for the HBoVs and other bocaparvoviruses. This study identifies three strain-specific antigenic epitopes on the HBoV1 capsid and a cross-reactive epitope on the HBoV1, HBoV2, and HBoV4 capsids using structures of capsid-antibody complexes determined using cryo-electron microscopy and image reconstruction. This is the first study to report the highly conserved parvovirus DE loop at the 5-fold axis as a determinant of antigenicity. Additionally, knowledge of the strain-specific and conserved antigenic epitopes of the bocaviruses can be instrumental in characterization of the virus life cycle, development of peptide vaccines, and generation of gene delivery vectors for cystic fibrosis given the strict tropism of HBoV1 for human airway epithelial cells.

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Seroepidemiology of human bocavirus in Apulia, Italy

Cited by 22 publications

References 20 publications

Detection of Human Bocavirus in Egyptian Children Suffering from Acute Lower Respiratory Tract Infection

Detection of Human Bocavirus in Egyptian Children Suffering from Acute Lower Respiratory Tract Infection

Frequent Detection and Genetic Diversity of Human Bocavirus in Urban Sewage Samples

Mapping Antigenic Epitopes on the Human Bocavirus Capsid

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