Serodiagnosis of Trypanosoma cruzi Infection Using the New Particle Gel Immunoassay - ID-PaGIA Chagas

Rabello, Ana; Luquetti, Alejandro O.; Moreira, Eliana Furtado; Gadelha, Maria de Fátima S.; Santos, José Alisson dos; Melo, Laércio de; Schwind, Peter

doi:10.1590/s0074-02761999000100016

Cited by 26 publications

(23 citation statements)

References 8 publications

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“…The screening used was the ID-PaGIA antibody test (Rabello et al 1999), and confirmation of positive samples was done by two different ELISA tests. The results yielded 21 positive samples by screening (1.2%), of which 11 were confirmed by ELISA (0.62%).…”

Section: Discussionmentioning

confidence: 99%

Epidemiology of Chagas disease in non endemic countries: the role of international migration

Schmuñis

2007

Mem. Inst. Oswaldo Cruz

473

290

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Section: Discussionmentioning

confidence: 99%

Epidemiology of Chagas disease in non endemic countries: the role of international migration

Schmuñis

2007

Mem. Inst. Oswaldo Cruz

473

290

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“…Crossreactivity to T. cruzi antigens has been detected in serum from patients with leishmaniasis (Chiller et al, 1990) or Trypanosoma rangeli infection (Guhl et al, 1987). In order to avoid false positives, the use of recombinant antigens and/or synthetic peptides has been proposed with success (Moncayo and Luquetti, 1990;Carvalho et al, 1993;Pastini et al, 1994;Peralta et al, 1994;Houghton et al, 1999 andUmezawa et al, 1999;Oelemann al., 1999;SaezAlquezar et al, 2000;Rabelo et al, 1999;Franco da Silveira et al, 2001). …”

Section: Introductionmentioning

confidence: 99%

Chagas’ disease diagnosis: a multicentric evaluation of Chagas Stat-Pak, a rapid immunochromatographic assay with recombinant proteins of Trypanosoma cruzi

Luquetti

Ponce

et al. 2003

Diagnostic Microbiology and Infectious Disease

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“…Diagnosis of chagasic patients has been made through parasitological and/ or serological methods like indirect hemagglutination (IH), indirect immunofluorescence (IFA) and indirect enzymelinked immunosorbent assay (ELISA). Other reactions such as chemiluminescence 1 , agglutination in gel 12 , lysis mediated by complement 8 and trans-sialidase inhibition 3 10 can be used. Chemiluminescence has not, however, been used for the diagnosis of Chagas' disease as it is still being standardized.…”

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confidence: 99%

Assessment of chemiluminescence and PCR effectiveness in relation to conventional serological tests for the diagnosis of Chagas' disease

Duarte¹,

Andrade

Monte

et al. 2006

Rev. Soc. Bras. Med. Trop.

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While testing 414 sera for the diagnosis of Chagas' disease, the conventional reactions of indirect hemagglutination, indirect immunofluorescence and the immunosorbent assay showed a sensitivity of 95.7%, 100% and 98.2% and a specificity of 98%, 98% and 96.4%, respectively, and an excellent association using Fisher's exact test. Chemiluminescence presented 100% sensitivity and 89.6% specificity, while PCR showed 100% specificity and 1.2% sensitivity. It is believed that the three conventional serological reactions are still adequate for diagnosing Chagas' disease. Key-words: Chagas' disease. Diagnosis. Serology. Chemiluminescence. PCR. Chagas' disease or American trypanosomiasis is an endemic infection of gradual evolution caused by the intracellular protozoa Trypanosoma cruzi. Diagnosis of chagasic patients has been made through parasitological and/ or serological methods like indirect hemagglutination (IH), indirect immunofluorescence (IFA) and indirect enzymelinked immunosorbent assay (ELISA). Other reactions such as chemiluminescence 1 , agglutination in gel 12 , lysis mediated by complement 8 and trans-sialidase inhibition 3 10 can be used. Chemiluminescence has not, however, been used for the diagnosis of Chagas' disease as it is still being standardized. RESUMOCurrent legislation 11 recommends screening tests for Chagas' disease in blood banks, but not in laboratories, using at least two methods with different methodological principles due to the false-positive and false-negative reactions found in different methodologies. IH, IFA and ELISA are considered typical reactions and are currently used. Technical scientific advances have made new tests and methods available. The suggestion to evaluate chemiluminescence and PCR as complementary tests for introduction in laboratory routines for the diagnosis of Chagas' disease, as well as their comparison with conventional methodologies is relevant, because of the need to find out precisely what their specificity, sensitivity and efficiency are. These three aspects are of major importance as they reflect not only on the results, but also on the prospect of clarifying doubts that have been raised by 386Duarte AMV et al cross-reactions with antibodies from carriers of chronic diseases and also the confirmation of indeterminate results obtained in conventional serological reactions.A total of 414 volunteers, male and female, aged between 10 and 80 years old, residents of urban and rural areas, took part in this study, which was conducted between October 2000 and January 2002. These individuals came from the municipalities of Itabira (n = 82), ferros (n = 229) and carbonita (n = 103) in the state of Minas Gerais, Brazil. Ninety-nine individuals had characteristic symptoms of the infection, such as cardiological (n = 79), digestive (n = 15) and cardiodigestive alterations (n = 5), in addition to being seropositive. Sixty-three volunteers showed no symptoms, even though they were seropositive, and eighteen experienced no symptoms and their sera were classified a...

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Serodiagnosis of Trypanosoma cruzi Infection Using the New Particle Gel Immunoassay - ID-PaGIA Chagas

Abstract: The ID-Chagas test is a particle gel immunoassay (PaGIA)

Cited by 26 publications

References 8 publications

Epidemiology of Chagas disease in non endemic countries: the role of international migration

Epidemiology of Chagas disease in non endemic countries: the role of international migration

Chagas’ disease diagnosis: a multicentric evaluation of Chagas Stat-Pak, a rapid immunochromatographic assay with recombinant proteins of Trypanosoma cruzi

Assessment of chemiluminescence and PCR effectiveness in relation to conventional serological tests for the diagnosis of Chagas' disease

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