Serodiagnosis of Toxocaral Larval Migrans in Monkeys by Enzyme-Linked Immunosorbent Assay (ELISA) with Somatic Adult and Secretory Larval Antigens

Knapen, F. van; Leusden, J. van; Buys, Janny

doi:10.2307/3281013

Cited by 8 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To detect antibodies, a wide variety of techniques have been utilized: larval microprecipitation test (OLSON, 1960); radioimmunoprecipitation (MAIZELS & MEGHI, 1984); indirect immunofluorescence (BAUFINE-DUCROCO etal., 1974); latex agglutination (SUGANE & OSHIMA, 1983). Since the isolation of a larval excretory-secretory antigen by D E SAVIGNY (1975), the chosen technique is by ELISA (VAN KNAPEN et al, 1982YANG et al, 1982;SMITH et al, 1982;CARLIER et al, 1982;MATSUMURA & ENDO, 1982;TONZ et al, 1983;JENKINS & RICKARD, 1984;SPEISER & GOTTSTEIN, 1984;REE et al, 1984;KAYES et al, 1985;GLICKMAN et al, 1985;GUILLEN et al, 1986a;BOWMAN et al, 1987). However, no agreement exists among different authors as to how specific this technique is.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of chemotherapy in experimental toxocarosis by determination of specific immune complexes

et al. 1990

View full text Add to dashboard Cite

Parasitism by the larval phase ofToxocara canisis a chronic process in which the larvae survive in the tissues, resulting in the constant stimulation of the immune system. As a result, the detection of specific antibodies may not reflect the active state of the parasite. We have studied the dynamics of the production of specific immune complexes by ELISA with the monoclonal antibody TC-1 in rabbits inoculated with single and multiple doses ofT. caniseggs. We also compared this with the production of specific antibodies and their possible modification after treatment with mebendazole. The specific antibodies against excretory-secretory antigen were detected with peaks at 10 and 12 weeks depending on the dose and remained positive during the entire experiment (62 weeks). Treatment caused an increase in the level of detectable antibodies dropping to similar levels to the controls. Specific immune complexes were detected only in multiple doses, and were then positive during the entire experiment. From the beginning of treatment the values of immune complexes fell quickly, remaining at undetectable levels during the rest of the experiment. For this reason the detection of specific immune complexes is a valid technique for monitoring the efficiency of treatment.

show abstract

Section: Introductionmentioning

confidence: 99%

Evaluation of chemotherapy in experimental toxocarosis by determination of specific immune complexes

et al. 1990

View full text Add to dashboard Cite

show abstract

Enzyme-linked immunosorbent assay using excretory/secretory and somatic antigens as a diagnostic test for human hookworm infection

Dilawari

Virk

et al. 1988

Transactions of the Royal Society of Tropical Medicine and Hygi

View full text Add to dashboard Cite

Excretory/secretory (E/S) and somatic antigens prepared from third stage infective larvae of Ancylostoma duodenale were evaluated for the diagnosis of human hookworm infection by enzyme-linked immunosorbent assay (ELISA). Taking the mean absorbance plus 2 standard deviations (SD) of normal serum as the cut-off value, the positivity rates in 30 hookworm cases were 97% and 93% respectively. False positivity with E/S antigen was 8-20% in 61 control subjects; with somatic antigen it was slightly lower. In contrast, when the mean absorbance value plus 2 SD of control groups with other parasitic infections was taken as cut-off point, the positivity was 93% with E/S antigen and 43% with somatic antigen, indicating the superiority of E/S antigen. Percentage positivity declined after treatment of hookworm cases. No relationship was found between ELISA reactivity and severity of infection.

show abstract

Detection of antibodies to secretions of Ancylostoma caninum in human eosinophilic enteritis

Loukas

Croese

Opdebeeck

et al. 1992

Transactions of the Royal Society of Tropical Medicine and Hygi

View full text Add to dashboard Cite

To evaluate the role of canine hookworms in human eosinophilic enteritis (EE) in north-eastern Australia, we tested human sera in an enzyme-linked immunosorbent assay (ELISA) which incorporated antigens of adult Ancylostoma caninum. Sera from the following groups were examined: 10 patients with EE (unexplained recurrent abdominal pain and related symptoms, with peripheral eosinophilia) from Townsville and Brisbane; 2 persons known to be infected with A. caninum and 20 presumed unexposed healthy controls; 20 patients with other gastrointestinal diseases; 20 with other identified parasitic infections; and 20 with atopic conditions. High levels of specific immunoglobulin (Ig) G and IgE antibodies were found in patients with EE but not other gastrointestinal disease. Excretory-secretory (ES) products were more discriminating than somatic antigens in the ELISA and the IgG/ES-ELISA was the most specific; occasional cross-reactions could be explained on epidemiological or parasitological grounds. The IgM-ELISA was neither specific nor sensitive. We conclude that canine ancylostomiasis is a major cause of human EE in north-eastern Australia, and the pathogenesis is based probably on hypersensitivity to antigens secreted by the parasite.

show abstract

Serodiagnosis of Toxocaral Larval Migrans in Monkeys by Enzyme-Linked Immunosorbent Assay (ELISA) with Somatic Adult and Secretory Larval Antigens

Cited by 8 publications

References 0 publications

Evaluation of chemotherapy in experimental toxocarosis by determination of specific immune complexes

Evaluation of chemotherapy in experimental toxocarosis by determination of specific immune complexes

Enzyme-linked immunosorbent assay using excretory/secretory and somatic antigens as a diagnostic test for human hookworm infection

Detection of antibodies to secretions of Ancylostoma caninum in human eosinophilic enteritis

Contact Info

Product

Resources

About