Serodiagnosis of Syphilis by an Enzyme‐Linked Immunosorbent Assay for IgG Antibodies Against the Reiter Treponeme Flagellum

Pedersen, Niels Tinggaard; Petersen, C S; Vejtorp, Mogens; Axelsen, N. H.

doi:10.1111/j.1365-3083.1982.tb00657.x

Cited by 68 publications

(33 citation statements)

References 17 publications

(13 reference statements)

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“…Because these organelles are not readily accessible to the ambient environment, they do not interact with flagellar-specific antibodies and hence circumvent the immobilization of the intact organism (13,15,30). Such antibodies often arise during infection (64,103). Furthermore, their intracellular location may also offer protection from possibly disruptive extremes in the environment such as pH or salt concentration (36).…”

Section: Selective Advantages Of Spirochetesmentioning

confidence: 97%

Genetics of Motility and Chemotaxis of a Fascinating Group of Bacteria: The Spirochetes

2002

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Spirochetes are a medically important and ecologically significant group of motile bacteria with a distinct morphology. Outermost is a membrane sheath, and within this sheath is the protoplasmic cell cylinder and subterminally attached periplasmic flagella. Here we address specific and unique aspects of their motility and chemotaxis. For spirochetes, translational motility requires asymmetrical rotation of the two internally located flagellar bundles. Consequently, they have swimming modalities that are more complex than the well-studied paradigms. In addition, coordinated flagellar rotation likely involves an efficient and novel signaling mechanism. This signal would be transmitted over the length of the cell, which in some cases is over 100-fold greater than the cell diameter. Finally, many spirochetes, including Treponema, Borrelia, and Leptospira, are highly invasive pathogens. Motility is likely to play a major role in the disease process. This review summarizes the progress in the genetics of motility and chemotaxis of spirochetes, and points to new directions for future experimentation.

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Section: Selective Advantages Of Spirochetesmentioning

confidence: 97%

Genetics of Motility and Chemotaxis of a Fascinating Group of Bacteria: The Spirochetes

2002

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“…These sera all had shown to have very high IgM and/or IgG antibody levels (absorbance >1.5) in the Reiter treponeme flagellum ELISA [17,18]. All sera were positive in WR, RPR and the FTA-absorption test.…”

Section: Control Seramentioning

confidence: 99%

Analysis of the human antibody response to outer surface protein C (OspC) of Borrelia burgdorferi sensu stricto, B. garinii, and B. afzelii

Mathiesen

Hansen

Axelsen

et al. 1996

Med Microbiol Immunol

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The aim of this study was to determine by Western blotting (WB) the prevalence of anti-outer surface protein C (OspC) IgM and IgG antibodies in patients with Lyme borreliosis according to each of the three genospecies of Borrelia burgdorferi sensu lato. Strains of B. burgdorferi sensu stricto (MUL), B. garinii (DK 6), and B. afzelii (DK 26) served as antigen, all of which expressed abundant OspC. We examined sera from 117 patients with untreated early and late Lyme borreliosis, as well as from 100 blood donors and 29 patients with syphilis. WB results were compared with the B. burgdorferi flagellum enzyme-linked immunosorbent assay (ELISA) data. OspC from B. burgdorferi sensu stricto showed the lowest diagnostic sensitivity. OspC from B. garinii and B. afzelii performed almost identically in erythema migrans, with an IgM positive rate of 36% versus 34%, whereas OspC from B. garinii performed best in neuroborreliosis (60% versus 44%). The anti-OspC IgG response was less prominent than the IgM response and was infrequent in the late stages of the disease (0-20%). The benefit of combining the evaluation of anti-OspC responses with all three species was limited. The overall diagnostic sensitivity of WB anti-B. garinii OspC evaluation was, in the early stages of the disease, comparable to the results obtained using the flagellum ELISA. In erythema migrans and neuroborreliosis, the addition of anti-OspC IgM to the flagellum ELISA increased the sensitivity by 15% and 10%, respectively. It can, therefore, be concluded that OspC from B. garinii is a suitable OspC test antigen, and that supplementary use of OspC from other species adds little to the overall diagnostic sensitivity. An ELISA based on B. garinii OspC and native flagella seems currently the most promising concept for a future antibody test in early Lyme borreliosis.

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“…Finally, because PFs are major immu-* Corresponding author. nogens in syphilis (3) and Lyme disease (28), seroreactivity to PF antigens has been used in disease diagnosis (34,41,52).…”

mentioning

confidence: 99%

Molecular genetic analysis of a class B periplasmic-flagellum gene of Treponema phagedenis

et al. 1992

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Treponema phagedenis is a host-associated spirochete with multiple polypeptides making up its periplasmic flagella (PFs). Each PF has a 39-kDa polypeptide making up the sheath (class A PF polypeptide) and two to four antigenically similar 33-to 34-kDa polypeptide species making up the core (class B PF polypeptides). A genetic analysis of the PF-deficient mutants T-40 and T-55 has shown that the PFs are involved in motility. To

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Serodiagnosis of Syphilis by an Enzyme‐Linked Immunosorbent Assay for IgG Antibodies Against the Reiter Treponeme Flagellum

Cited by 68 publications

References 17 publications

Genetics of Motility and Chemotaxis of a Fascinating Group of Bacteria: The Spirochetes

Genetics of Motility and Chemotaxis of a Fascinating Group of Bacteria: The Spirochetes

Analysis of the human antibody response to outer surface protein C (OspC) of Borrelia burgdorferi sensu stricto, B. garinii, and B. afzelii

Molecular genetic analysis of a class B periplasmic-flagellum gene of Treponema phagedenis

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