Analysis of the human antibody response to outer surface protein C (OspC) of Borrelia burgdorferi sensu stricto, B. garinii, and B. afzelii

Mathiesen, Marianne J.; Hansen, Klaus; Axelsen, N. H.; Halkier-Sørensen, L; Theisen, Michael

doi:10.1007/s004300050021

Cited by 24 publications

(22 citation statements)

References 29 publications

(34 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As a signi®cant heterogeneity has been described for OspC in European strains [35,38,39], one might reason that in this study an immune response to OspC and/or Osp17 was detected only in patients with B. afzelii infection. However, concerning OspC, we found that the IgM response is primarily directed against conserved epitopes [37], a ®nding con®rmed also by Mathiesen et al [23]. For Osp17, in can not be ruled out that heterogeneity may play a greater role compared to OspC.…”

Section: Lymphoproliferation To Lysate Antigenssupporting

confidence: 69%

Characterization of the cellular and humoral immune response to outer surface protein C and outer surface protein 17 in children with early disseminated Lyme borreliosis

Pohl‐Koppe

Kaunicnik

Wilske³

2001

Med Microbiol Immunol

View full text Add to dashboard Cite

OspC and Osp17 are immunodominant proteins of Borrelia burgdorferi eliciting a clear humoral immune response in adult patients with systemic Lyme disease. In this study, the cellular immune response to B. burgdorferi and the major outer surface proteins OspC and Osp17 was investigated in children during the course of early disseminated B. burgdorferi infection. Lymphoproliferative responses to recombinant proteins were compared to the protein-specific humoral immune reaction. OspC induced a clear antibody response but elicited an even stronger cellular immune response. In contrast, a cellular as well as humoral immune reaction to Osp17 was only rarely detected. Follow-up examinations demonstrated that the lymphoproliferative response to B. burgdorferi and OspC persisted for several months after antibiotic therapy. Here, we show that in early disseminated Lyme disease of childhood, OspC is a potent antigen influencing both the humoral and cellular immunity, while Osp17 plays only a minor role in immune activation.

show abstract

Section: Lymphoproliferation To Lysate Antigenssupporting

confidence: 69%

Characterization of the cellular and humoral immune response to outer surface protein C and outer surface protein 17 in children with early disseminated Lyme borreliosis

Pohl‐Koppe

Kaunicnik

Wilske³

2001

Med Microbiol Immunol

View full text Add to dashboard Cite

show abstract

“…Mathiesen et al, who reported also that OspC of the B. garinii strain utilized by them was as sensitive alone as in combination with the OspCs of the representatives of the two other species (28). However, in an evaluation of IgG WBs with recombinant OspCs derived from the three species, OspC of PKo was the most sensitive and OspC of PBi was the least sensitive (42).…”

Section: Discussionmentioning

confidence: 99%

Diagnostic Value of Proteins of ThreeBorreliaSpecies (Borrelia burgdorferiSensu Lato) and Implications for Development and Use of Recombinant Antigens for Serodiagnosis of Lyme Borreliosis in Europe

Hauser

Lehnert

Wilske

1998

Clin Diagn Lab Immunol

View full text Add to dashboard Cite

More and more assays for the serodiagnosis of Lyme borreliosis (LB) are based on recombinant antigens. However, so far, there is no consensus as to which are the most specific and sensitive proteins and how they should be used in combination to obtain tests with the best discrimination abilities. The present study was preceded by a detailed analysis of Western blots (WB) using whole-cell lysates ofBorrelia burgdorferi sensu stricto strain PKa2, B. afzelii PKo, and B. garinii PBi (U. Hauser, G. Lehnert, R. Lobentanzer, and B. Wilske, J. Clin. Microbiol. 35:1433–1444, 1997). For the present work, the data bank from that study, containing information about the reactivities of 330 sera (from patients at different stages of LB [n = 189]; control group, n = 141), was reused. The specificities and sensitivities of various combinations of proteins from different strains were calculated for different interpretation criteria. For immunoglobulin G (IgG) WB, the recommended combination of antigens available to date as recombinant proteins included p83/100 of PKa2, p83/100 of PKo, p39 of PKo, p39 of PBi, and OspC of PBi (interpretation criterion, at least one reactive band required for a positive WB; specificity, 96.5%; sensitivity, 56.1%). The further addition of p58 of PKo, p17 of PKo, or p14 of PKo was most favorable in terms of both a considerable gain of sensitivity and little loss of specificity. IgG Western blotting with a whole-cell lysate of strain PKo might be improved by the addition of OspC of PBi. For IgG WB, the best combination, out of all bands, was p83/100, p58, p39, p30, and p21 of all three strains and OspC of PBi, p17b of PBi, p56 of PKa2, p43 of PKo, p17 of PKo, and p14 of PKo (interpretation criterion, at least two reactive bands required for a positive WB; specificity, 97.2%; sensitivity, 61.4%). An interpretation criterion of at least two reactive bands is more reliable than one of only one reactive band. For IgM WB, the best combination was OspC of PKo, OspC of PBi, p39 of all three strains, p17 of PKo, and strong reactions with p41 of all three strains (interpretation criterion, at least one reactive band required; specificity, 97.9%; sensitivity, 47.0%).

show abstract

“…Highly passaged in vitro-cultured B. burgdorferi sensu lato does not express OspC, which explains why the importance of this antigen was unrecognized in early studies that used high-passage B. burgdorferi sensu lato as the source of antigen (63,70). OspC is heterogeneous, and amino acid differences exist among the sequences of OspC proteins from the different B. burgdorferi sensu lato species (135,197,336). Furthermore, intraspecies differences also exist; for example, at least 13 OspC serotypes have been identified in B. garinii by using a panel of monoclonal antibodies (361).…”

Section: Immunologic Diagnosis Of B Burgdorferi Sensu Lato Infectionmentioning

confidence: 99%

“…For example, OspC of B. garinii strains has better immunoreactivity than OspC of other B. burgdorferi sensu lato species in detecting IgM antibodies in patients with neuroborreliosis. This is most likely explained by the fact that B. garinii is the species that most frequently causes neuroborreliosis in Europe (197).…”

Section: Immunologic Diagnosis Of B Burgdorferi Sensu Lato Infectionmentioning

confidence: 99%

Diagnosis of Lyme Borreliosis

et al. 2005

View full text Add to dashboard Cite

A large amount of knowledge has been acquired since the original descriptions of Lyme borreliosis (LB) and of its causative agent, Borrelia burgdorferi sensu stricto. The complexity of the organism and the variations in the clinical manifestations of LB caused by the different B. burgdorferi sensu lato species were not then anticipated. Considerable improvement has been achieved in detection of B. burgdorferi sensu lato by culture, particularly of blood specimens during early stages of disease. Culturing plasma and increasing the volume of material cultured have accomplished this. Further improvements might be obtained if molecular methods are used for detection of growth in culture and if culture methods are automated. Unfortunately, culture is insensitive in extracutaneous manifestations of LB. PCR and culture have high sensitivity on skin samples of patients with EM whose diagnosis is based mostly on clinical recognition of the lesion. PCR on material obtained from extracutaneous sites is in general of low sensitivity, with the exception of synovial fluid. PCR on synovial fluid has shown a sensitivity of up to >90% (when using four different primer sets) in patients with untreated or partially treated Lyme arthritis, making it a helpful confirmatory test in these patients. Currently, the best use of PCR is for confirmation of the clinical diagnosis of suspected Lyme arthritis in patients who are IgG immunoblot positive. PCR should not be used as the sole laboratory modality to support a clinical diagnosis of extracutaneous LB. PCR positivity in seronegative patients suspected of having late manifestations of LB most likely represents a false-positive result. Because of difficulties in direct methods of detection, laboratory tests currently in use are mainly those detecting antibodies to B. burgdorferi sensu lato. Tests used to detect antibodies to B. burgdorferi sensu lato have evolved from the initial formats as more knowledge on the immunodominant antigens has been collected. The recommendation for two-tier testing was an attempt to standardize testing and improve specificity in the United States. First-tier assays using whole-cell sonicates of B. burgdorferi sensu lato need to be standardized in terms of antigen composition and detection threshold of specific immunoglobulin classes. The search for improved serologic tests has stimulated the development of recombinant protein antigens and the synthesis of specific peptides from immunodominant antigens. The use of these materials alone or in combination as the source of antigen in a single-tier immunoassay may someday replace the currently recommended two-tier testing strategy. Evaluation of these assays is currently being done, and there is evidence that certain of these antigens may be broadly cross-reactive with the B. burgdorferi sensu lato species causing LB in Europe

show abstract

Analysis of the human antibody response to outer surface protein C (OspC) of Borrelia burgdorferi sensu stricto, B. garinii, and B. afzelii

Cited by 24 publications

References 29 publications

Characterization of the cellular and humoral immune response to outer surface protein C and outer surface protein 17 in children with early disseminated Lyme borreliosis

Characterization of the cellular and humoral immune response to outer surface protein C and outer surface protein 17 in children with early disseminated Lyme borreliosis

Diagnostic Value of Proteins of ThreeBorreliaSpecies (Borrelia burgdorferiSensu Lato) and Implications for Development and Use of Recombinant Antigens for Serodiagnosis of Lyme Borreliosis in Europe

Diagnosis of Lyme Borreliosis

Contact Info

Product

Resources

About