Serodiagnosis of sheep hydatidosis with hydatid fluid, protoscolex, and whole body of Echinococcus granulosus antigens

Tabar, Gholamreza Hashemi; Haghparast, Alireza; Borji, Hassan

doi:10.1007/s00580-010-1112-4

Cited by 7 publications

(9 citation statements)

References 16 publications

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“…In general, different immunological responses, estimated either serologically or cytopathologically, have been reported. The mechanism of protective immunity depends on the antigenicity, the molecular size and the number of epitopes provided by the different antigens (WATTAL et al, 1986;TABAR et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…It is generally accepted that Echinococcus is unaffected by the immune response during the developing stage during primary infection. However, dead, calcified metacestodes or necrotic cysts have been reported in some cystic lesions in naturally infected sheep, indicating that the cysts can be killed during later stages of development (TABAR et al, 2012). The authors suggested that the immune response plays a key role in the death of the parasite, which may signify increased immunological stimulation upon cyst progression.…”

Section: Discussionmentioning

confidence: 99%

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Post-immunization immunohistochemical expression of Caspase 3 and p53 apoptotic markers in experimental hydatidosis

El-Aal

El-Gebaly

Al-Antably

et al. 2016

Rev. Bras. Parasitol. Vet.

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The aim of this study was to investigate post-immunization apoptotic changes in experimental hydatidosis, using Caspase 3 and p53 immunohistochemical markers. Two groups of rabbits were immunized with a crude antigen (group 1) or a partially purified antigen (group 2) and were compared to an infected non-immunized control group. More effective immune responses were obtained in group 2 than group 1, signified by fewer and smaller cystic lesions and more severe destructive changes. Normal growth of cysts was attained in the control group, with no expression of apoptotic markers. Significantly higher expression of Caspase 3 and p53 were observed in group 1 compared to group 2, as indicated by OD and area percentage, respectively (Group 1 Caspase 3: 0.89±0.21, 93.5%±6.2; Group 1 p53: 0.46±0.18, 53.26%±11.6; Group 2 Caspase 3: 0.52±0.15, 49.23%±11.7; Group 2 p53: 0.19±0.4, 18.17%±7.3). Vaccine-induced immune responses and cellular damage may underlie the expression of apoptotic markers that appeared to result in a degenerative and atrophic course of action upon immunization. The results of the current study emphasize the importance of immunization for the stimulation of protective immune responses and in preventing mechanisms of evasion to ensure normal cell growth. A cost/benefit control program that implements proper vaccine preparations should be further assessed for complete elimination of severe infections in endemic areas.Keywords: Experimental hydatidosis, Caspase 3, p53, apoptosis. ResumoO objetivo do presente estudo foi investigar mudanças de apoptose pós-imunização em hidatidose experimental, usando os marcadores imuno-histoquímicos Caspase 3 e p53. Dois grupos de coelhos foram imunizados com antígeno bruto (grupo 1) e com antígeno parcialmente purificado (grupo 2). Estes grupos foram comparados a um grupo controle infectado e não-imunizado. Respostas imunes mais eficientes foram obtidas do grupo 2, que apresentou lesões císticas menores e menos frequentes, e mudanças destrutivas mais graves. Cistos cresceram normalmente no grupo controle, sem expressão dos marcadores de apoptose. Expressões significativamente mais altas de Caspase 3 e p53 foram observadas no grupo 1 quando comparado ao grupo 2, como indicado por DO e área de percentagem, respectivamente (Grupo 1 Caspase 3: 0,89±0,21, 93,5%±6,2; Grupo 1 p53: 0, 46±0,18, 53,26%±11,6; Grupo 2 Caspase 3: 0,52±0,15, 49,23%±11,7; Grupo 2 p53: 0,19±0,4, 18,17%±7,3). Respostas imunológicas induzidas por vacinas e danos celulares podem ser a base para a expressão dos marcadores de apoptose cujos desfechos demonstraram ação degenerativa e atrófica durante imunização. Os resultados do presente estudo enfatizam a importância da imunização para o estímulo de respostas imunes de proteção e para mecanismos de prevenção de evasão para garantir crescimento celular normal. Um programa de controle de custo/benefício que implemente preparações de vacinas adequadas deve ser analisado em mais detalhe para a completa eliminação de infecções graves em áreas endêmicas.Pal...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Post-immunization immunohistochemical expression of Caspase 3 and p53 apoptotic markers in experimental hydatidosis

El-Aal

El-Gebaly

Al-Antably

et al. 2016

Rev. Bras. Parasitol. Vet.

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“…on the lamb model, the highest antibody level was produced using whole body of E. granulosus . Also, hydatid fluid had a remarkably protective level of antibody production (Hashemitabar & Razmi, 2009). Concerning the use of crude antigens, Kandil et al .…”

Section: Discussionmentioning

confidence: 99%

“…The oncospheral antigen, EG95, sheep vaccine is a well‐known representative, assisting the elimination of CE transmission with promising clinical trials conducted in China and South America (Lightowlers & Heath, 2004; Heath, Robinson & Lightowlers, 2012; Heath, Robinson, Shakes, et al, 2012; Larrieu et al., 2013; Larrieu et al., 2015). As for CE vaccination, the initial strategy is based on the crude somatic or excretory/secretory (E/S) antigens extracted from various stages of E. granulosus life cycle, including the whole body of an adult worm, crude extracts of protoscolex, brood capsules and adult worm tissues (Hashemitabar & Razmi, 2009). Besides, the next‐generation multimodal vaccines such as nano‐scale gene‐based vaccines can be specifically used for triggering the mucosal immune system and generating long‐lasting protection (Hagan, Nakaya, Subramaniam, & Pulendran, 2015; Hu et al., 2015).…”

Section: Introductionmentioning

confidence: 99%

Current situation and future prospects of Echinococcus granulosus vaccine candidates: A systematic review

Anvari

Rezaei

Ashouri

et al. 2020

Transbound Emerg Dis

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Cystic echinococcosis is a worldwide zoonotic disease, represents a threat for livestock and humans, manifests as a quiescent, subclinical and chronic hydatid cyst infection. The disease imposes high expenditures and economic losses in medical and veterinary. Prophylactic vaccination would be one of the effective preventive health care against echinococcosis. During the last decades, many studies have characterized the protective antigens of Echinococcus granulosus and their role in immunization of various animal host species. Herein, we aimed to systematically evaluate and represent the best antigens as possible vaccine candidates for cystic echinococcosis.Data were systematically searched from five databases including ProQuest, PubMed, Scopus, ScienceDirect and Web of Science, up to 1 February 2020. Two reviewers independently screened and assessed data extraction and quality assessment. A total of 47 articles were eligible for inclusion criteria in the current study. The most common antigens used for vaccination against E. granulosus were EG95 and antigen B.Freund's adjuvant and Quil A have been predominantly utilized. In addition, regarding the antigen delivery, animal models, measurement of immune responses and reduction in hydatid cyst have been discussed in the text. The data demonstrated that DNA vaccines with antigen B and recombinant protein vaccines based on EG95 antigen have the best results and elicited protective immune responses.

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“…Hydatidosis or cystic echinococcosis (CE, hydatid cyst) is one of the serious silent cyclozoonotic diseases between humans and domestic animals caused by larval stage of Echinococcus granulosus [1]. This disease with worldwide distribution is more seen in Australia, South America, Mediterranean and the Middle East countries.…”

Section: Introductionmentioning

confidence: 99%

Scolicidal Effects of Gallic Acid, One of the Major Compounds of Plants, on Protoscolices of Hydatid Cyst

Larki

Jalali

Goodarzi

2017

Zahedan J Res Med Sci

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Background: Today's, the recurrent of hydatidosis remain as a concern in many surgeries. Exposure of a suitable effective scolicidal agent with protoscolex-rich fluid can prevent these complications. In recent studies more have been considered to importance of natural products particularly herbal medcines to evaluate their scolicidal effects. Gallic acid a type of organic acid is one of the main known effective biological phenolic substances of plants. Objectives: The aim of this study was to evaluate the effect of Gallic acid on protoscolices of hydatid cysts in in vitro. Methods: In this experimental study, the protoscolices were obtained from liver hydatid cysts of infected sheep. Gallic acid solution was used at the concentration of 25, 30 and 35 mg/mL for 1, 3, and 5 minutes. The live protoscolices were detected by 0.1% eosin dye. Data analysis was performed by SPSS version 11.5 and one-way ANOVA test. Results:The Gallic acid at the concentration of 25 mg/mL disabled 35.65%, 52.21% and 65.38% of protoscolices after 1, 3 and 5 min of faced time together, respectively. Also, the scolicidal rate of Gallic acid at concentration of 30 mg/mL was 29.92%, 63.80% and 67.76% after 1, 3 and 5 min, respectively. It was observed that concentration of 35 mg/mL Gallic acid on protoscolices caused 92.08% and 100% mortality rate of parasites after 1 and 3 minutes, respectively (compared with 9.9% in control groups). Conclusions:The findings of present study showed that Gallic acid as an effective constituent of some herbal scolicidal agent can be considered as responsible for killing of protoscolices. However, further studies will be needed to confirm this phytochemicals active component in animal models.

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Serodiagnosis of sheep hydatidosis with hydatid fluid, protoscolex, and whole body of Echinococcus granulosus antigens

Cited by 7 publications

References 16 publications

Post-immunization immunohistochemical expression of Caspase 3 and p53 apoptotic markers in experimental hydatidosis

Post-immunization immunohistochemical expression of Caspase 3 and p53 apoptotic markers in experimental hydatidosis

Current situation and future prospects of Echinococcus granulosus vaccine candidates: A systematic review

Scolicidal Effects of Gallic Acid, One of the Major Compounds of Plants, on Protoscolices of Hydatid Cyst

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