Serodiagnosis of Oesophagostomum Columbianum infection in Goats using indirect ELISA

Abstract

To determine the seroprevalence of Oesophagostomum columbianum antibodies in goats in Kashmnir, a study was conducted in Srinagar and Ganderbal Districts, located in central parts of Kashmir valley. ELISA was standardized and evaluated to detect goat Oesophagostomosis in experimental and clinical cases using somatic whole adult antigen of Oesophagostomum columbianum. Plate ELISA was standardized using 5µg/well antigen concentration with 1:100 and 1:1000 of sera and conjugate dilution. Indirect plate ELISA was able to demonstrate the antibody titer at different weeks post infection in experimental goats. A comparison of plate ELISA on suspected field sera and faecal sample examination by floatation method revealed that 68 (34.34%) samples were found to be positive using ELISA and twelve by faecal examination. The highest prevalence was found in lower age groups in both the seasons, with the increase in age, the infection level decreased. The prevalence was higher in wet season as compared to dry season. The results were stastically significant (P < 0.05). No sex predisposition was seen. Sensitivity and specificity of ELISA was 80.0%, and 21.42%, respectively. This test is therefore quite sensitive for clinical cases; an early diagnosis, however lacks specificity of faecal examination were 10.81% and 90.0%, respectively.

Cross antigenicity of immunodominant polypeptides of somatic antigen of Oesophagostomum columbianum with other helminths by western blotting

“…columbianum with. H. contortus was reported using DID and similar results employing ELISA [ 4 ]. Surface reactivity patterns among strongylid nematodes using immunoperoxidase assay revealed cross antigenicity of O. columbianum with H .…”

“…Clinical symptoms of these infections are overlapping making specific diagnosis difficult. Although, studies on immunodiagnosis of O. columbianum was reported previously by few worker employing ELISA and double immunodiffusion (DID) test [ 4 - 8 ]. Antigenic cross-reactivity of O .…”

Influence of Massive and Long Distance Migration on Parasite Epidemiology: Lessons from the Great Wildebeest Migration

“…All four helminths recovered from the gastrointestinal tract of the migratory wildebeests had relatively low infection intensities and, quite surprisingly, were generalist species that commonly infect livestock (Dube et al 2004;Tariq et al 2008). Helminths recovered from migrating wildebeest in this study could result from cross-infection from the domestic reservoirs along pastoral landscapes that dominate the wildebeest migratory routes (Dash 1973;Nwosu et al 1996, Lone et al 2011Junker et al 2014). The relative rarity of C. raja may be related to the scarcity of swampy areas required for the survival of intermediate snail host on the migratory pathways of wildebeest.…”