Cross antigenicity of immunodominant polypeptides of somatic antigen of Oesophagostomum columbianum with other helminths by western blotting

Abstract

Aim:Oesophagostomum columbianum in small ruminants in India is found as mixed infection commonly in sheep and goat. Haemonchus contortus, an abomasal nematode is found as concurrent infection with it. Eggs of Haemonchus and O. columbianum cannot be easily distinguished. Diagnosis of O. columbianum may only be possible if a non-cross antigenic polypeptide was available for immunodiagnosis.Materials and Methods:Somatic antigen (SoAg) of O. columbianum was fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunodominant polypeptides were identified by western blotting with homologous hyperimmune serum (HIS) and experimental sera of sheep or goat infected with other helminths.Results:SoAg of O. columbianum was immunoaffinity purified. Sharp polypeptide bands of 130, 72 and 68 KDa were observed along with several faint bands of lower molecular weight. Western blot of purified SoAg of O. columbianum with homologous HIS showed reaction with all the protein bands of 17, 28, 30, 32, 35, 38, 50, 68, 100, 130, 150, and 170 kDa. For identification of non-cross antigenic polypeptide, immunoaffinity purified SoAg of O. columbianum was reacted to heterologous HIS against H. contortus, Paramphistomum epiclitum, and Fasciola gigantica in western blotting utilizing completely dry method (i-blot). Among high molecular weight polypeptides 100 and 150 kDa were non-cross antigenic and among low molecular weight except 50 kDa polypeptide, 17, 30, 32, 35, and 38 kDa of O. columbianum were not cross antigenic with other helminths.Conclusions:Hence, polypeptides of 17, 30, 32, 35 and 38 kDa as well as 100 and 150 kDa polypeptides of O. columbianum may be exploited for immunodiagnosis of the infection in sheep and goat with extensive studies on cross antigenicity.

An Overview of Plants with Acaricidal and Anthelmintic Properties

“…Likewise, tick associated concerns as to their increasing prevalence, lowered animal productivity; their zoonotic potential and control have been considered as an important area for future research (Ashfaq et al, 2015;Jabbar et al, 2015;Zahid et al, 2016). Helminth infections are also ranked high among the factors leading to low production in animals throughout the world with varying prevalence depending on the climatic conditions, awareness of farmers on the recommended parasite control practices and accessibility to the animal health care (Khan et al, 2010b;Tasawar et al, 2010;Qamar et al, 2011;Borji et al, 2012;Katoch et al, 2012;Roeber et al, 2013;Singh et al, 2014;Bansal et al, 2015;Dalal et al, 2015;Lashari et al, 2015;Scala et al, 2015;Voigt et al, 2016).…”