Serodiagnosis of Neurocysticercosis

Schantz, Peter; Tsang, Victor C. W.; Maddison, Shirley E.

doi:10.1093/clinids/10.6.1231-b

Cited by 18 publications

(13 citation statements)

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“…Despite the high specificity of the WB test commonly observed by various authors 2,7,12,24,29,32 , crossreactions with other parasites may occur 18 . The wide divergence in the sensitivity (69.0 to 87.0%) of serologic tests observed by various authors 6,7,20 reflects differences not only in the methodology employed (antigen, reagents, techniques), but also in the characteristics of the population investigated 23 .…”

Section: Discussionmentioning

confidence: 98%

Frequency of serum anti-cysticercus antibodies in the population of a rural Brazilian community (Cássia dos Coqueiros, SP) determined by ELISA and immunoblotting using Taenia crassiceps antigens

Bragazza

Vaz

Passos

et al. 2002

Rev. Inst. Med. trop. S. Paulo

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Considering the impact of cysticercosis on public health, especially the neurologic form of the disease, neurocysticercosis (NC), we studied the frequency of positivity of anti-Taenia solium cysticercus antibodies in serum samples from 1,863 inhabitants of Cássia dos Coqueiros, SP, a municipal district located 80 km from Ribeirão Preto, an area considered endemic for cysticercosis. The 1,863 samples were tested by enzyme linked immunosorbent assay (ELISA) using an antigenic extract from Taenia crassiceps vesicular fluid (Tcra). The reactive and inconclusive ELISA samples were tested by immunoblotting. Of the 459 samples submitted to immunoblotting, 40 were strongly immunoreactive to the immunodominant 18 and 14 kD peptides. Considering the use of immunoblotting as confirmatory due to its high specificity, the anti-cysticercus serum prevalence in this population was 2.1%.

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Section: Discussionmentioning

confidence: 98%

Frequency of serum anti-cysticercus antibodies in the population of a rural Brazilian community (Cássia dos Coqueiros, SP) determined by ELISA and immunoblotting using Taenia crassiceps antigens

Bragazza

Vaz

Passos

et al. 2002

Rev. Inst. Med. trop. S. Paulo

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“…Therefore, the search for IgG antibodies to a crude extract by means of ELISA should be considered only as a screening test that could be of particular value in schistosomiasis-free and echinococcosis-free areas (7). However, the introduction of immunoblotting techniques may allow for species-specific immunodiagnosis (26), even through the use of crude extracts (27). Gottstein et al (15) have identified two polypeptides, with molecular weights of 26,000 and 8,000 recognized by CSF and serum antibodies from patients with NC but not patients with other helminthic infections.…”

Section: Qmentioning

confidence: 99%

Antibodies to the metacestode of taenia solium in the saliva from patients with neurocysticercosis

Acosta¹

1990

Clinical Laboratory Analysis

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IgG antibodies to antigens of Taenia solium metacestodes were detected in saliva samples from patients with intracerebral cysticercosis by means of an enzyme-linked immunosorbent assay (ELISA). When compared for IgG antibody activity, saliva samples from patients with various nonparasitic neurological disorders and from clinically healthy individuals yielded significantly lower (p less than 0.001) absorbance values than saliva samples from patients with neurocysticercosis. However, no differences were observed in IgA anti-T. solium activity between patients with neurocysticercosis and controls. These results indicate that the detection of anticysticercus IgG antibodies in saliva by means of ELISA may be of value in the diagnosis of neurocysticercosis. Moreover, collection of saliva provides a noninvasive sampling method for immunoepidemiological surveys on this disease.

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“…Experimental intraperitoneal murine cysticercosis is also well known as a source of cross-reacting antigens useful in immunodiagnosis of human cestode disease (4,5,6), and as a practical model to test candidate vaccines against porcine Taenia solium cysticercosis (7,8,9). It is also a manageable experimental system designed to explore the role of biological factors involved in host susceptibility (10,11,12).…”

Section: Introductionmentioning

confidence: 99%

Gonadectomy and progesterone treatment induce protection in murine cysticercosis

Vargas-Villavicencio¹,

Larralde²,

Morales‐Montor³

2006

Parasite Immunology

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The effects of progesterone on castrated mice of both sexes infected with Taenia crassiceps cysticerci were studied. Gonadectomy and treatment with progesterone before infection decreased parasite loads by 100% compared with intact uninfected mice. mRNA levels of IFN-gamma and IL-2 (typically associated to Th1-like profiles) were markedly decreased in infected gonadectomized (Gx) mice, whereas progesterone treatment of infected Gx mice did not affected its expression. mRNA levels of IL-4, and IL-10 (typically associated with Th2-like profiles) were reduced by gonadectomy, whereas restitution with progesterone did not affected this pattern in infected Gx progesterone-treated mice. Infection markedly induced expression of progesterone receptor isoform A in splenocytes of Gx mice (5-fold), whereas isoform B had no changes. Progesterone metabolism to dehydroepiandrosterone (DHEA) in Gx animals was increased 3-fold only in infected progesterone-treated uninfecteds of both sexes, but was not detectable in infected Gx progesterone-treated mice. Conversely, DHEA levels increased 100-fold in infected Gx progesterone-treated mice. However, androgen receptor expression in splenocytes of male mice showed a reduction by gonadectomy, and by infection, whereas in females AR expression showed no changes in the different mouse groups. These results suggest that progesterone, through its metabolism to DHEA, negatively affects the establishment, growth, and reproduction of Taenia crassiceps, by a mechanism that does not implicate a classic genomic pathway involving a nuclear androgen receptor.

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Serodiagnosis of Neurocysticercosis

Cited by 18 publications

References 0 publications

Frequency of serum anti-cysticercus antibodies in the population of a rural Brazilian community (Cássia dos Coqueiros, SP) determined by ELISA and immunoblotting using Taenia crassiceps antigens

Frequency of serum anti-cysticercus antibodies in the population of a rural Brazilian community (Cássia dos Coqueiros, SP) determined by ELISA and immunoblotting using Taenia crassiceps antigens

Antibodies to the metacestode of taenia solium in the saliva from patients with neurocysticercosis

Gonadectomy and progesterone treatment induce protection in murine cysticercosis

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