Frequency of serum anti-cysticercus antibodies in the population of a rural Brazilian community (Cássia dos Coqueiros, SP) determined by ELISA and immunoblotting using Taenia crassiceps antigens

Bragazza, Lúcia Maria; Vaz, Adelaide José; Passos, Afonso Dinis da Costa; Takayanagui, Osvaldo Massaiti; Nakamura, Paulo Mutuko; Espíndola, Noeli Maria; Pardini, Alessandra Xavier; Bueno, Ednéia Casagranda

doi:10.1590/s0036-46652002000100002

Cited by 26 publications

(23 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…ELISA tests showed sensibility of 100% in patients diagnosed with NC in our survey. The frequency of anti-T. solium cysticercus antibodies in the studied population is higher than those found by Bragazza et al (2002), using a similar methodology in a population of Cássia dos Coqueiros (2.1%), a rural area near Ribeirão Preto, São Paulo, also considered an endemic region for cysticercosis. Surveys by ELISA by Vaz et al (1990) in a population from five counties of São Paulo, and Silveira-Lacerda et al (2002) in blood donors from four cities in Minas Gerais found a frequency of anti-T. solium cysticercus antibodies of 2.3 and 5.6% respectively.…”

Section: Resultscontrasting

confidence: 54%

See 1 more Smart Citation

Taeniosis-cysticercosis complex in individuals of a peasants' settlement (Teodoro Sampaio, Pontal of Paranapanema, SP, Brazil)

Prestes-Carneiro

Freitas

Zago

et al. 2006

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

In order to evaluate the taeniosis-cysticercosis complex in a population of a peasants ' settlement, located at Teodoro Sampaio, state of São Paulo, Brazil (longitude 52°36'12", latitude 22°17'12") The taeniosis-cysticercosis complex is an important cause of morbidity and mortality, widespread not only in most underdeveloped countries, but also in industrialized ones (Raether & Hanel 2003). There is strong evidence supporting the high prevalence of Taenia infection in humans from resource-poor areas, especially in rural areas with deficient sanitation, low hygienic standards, and unusual customs, such as consumption of raw pork (Phiri et al. 2003).Taenia solium and Taenia saginata are two taenids of great economic and medical importance, causing bovine and porcine cysticercosis and taeniosis in humans. The encysted larva, known as cysticercus from T. saginata and T. solium are found in cattle and swine, respectively, and the adult tapeworm of T. saginata and T. solium are found in man. The cycle is completed when eggs in proglottids shed from the human tapeworm are disseminated to the environment through feces, followed by the ingestion by cattle or swine, and then the viable cysts are eaten by man in undercooked beef (Lees et al. 2002). People are also infected by ingestion of T. solium or T. saginata eggs by contact with carriers or contaminated foods. Cysticercosis is an infection caused by cysticercus, the larval stage of Cestoda Family Taeniidae. Neurocysticercosis (NC), the nervous-system form of cysticercosis is caused by larvae of tapeworm T. solium (Carpio 2002).In Brazil, several previous studies have shown that NC in humans is an important public-health problem; although, very few reports on the prevalence and distribution of porcine cysticercosis have been published (Sakai et al. 2001). In the state of São Paulo, the frequency of anti-T. solium cysticercus antibodies in humans was determined in few counties (Vaz et al. 1990, Bragazza et al. 2002. Nonetheless, studies about the prevalence of T. saginata infection in humans or the presence of T. saginata cysticercus in cattle are scarce (Ungar & Germano 1992). Dias et al. (1991) found in 311 samples of stools of humans living in the state of São Paulo an occurrence of 0.5% in Taenia sp. eggs; of which 273 were identified as T. saginata proglottidis.The ELISA screening test is commonly used for the serological diagnosis of cysticercosis, although it crossreacts with cestoda parasites such as Hymenolepis nana and Echinococcus granulosos (Garcia et al. 2003, Ishida et al. 2003. Low-molecular-weight glycoproteins obtained from T. solium by affinity chromatography, considerably improved the specificity of ELISA screening tests, but a large amount of antigens is required (Tsang et al. 1989). Recently, it has successfully been reported the use of low-molecular-weight peptides obtained from the vesicular fluid of T. crassiceps antigens (Tcra-VF) and T. crassiceps cysticercus glycoproteins (Tcra-GP and Tcra(18-14)-GP in ELISA and immunoblot assays , Ishida e...

show abstract

Section: Resultscontrasting

confidence: 54%

“…In the state of São Paulo, the frequency of anti-T. solium cysticercus antibodies in humans was determined in few counties (Vaz et al 1990, Bragazza et al 2002. Nonetheless, studies about the prevalence of T. saginata infection in humans or the presence of T. saginata cysticercus in cattle are scarce (Ungar & Germano 1992).…”

mentioning

confidence: 99%

Taeniosis-cysticercosis complex in individuals of a peasants' settlement (Teodoro Sampaio, Pontal of Paranapanema, SP, Brazil)

Prestes-Carneiro

Freitas

Zago

et al. 2006

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

show abstract

“…The population studied range in age from six to 81 years with the adults' serum samples being more reactive. The results agreed with other studies 1,3,5 , indicating that the higher prevalence among adults was presumably due to their longer period of exposure and/or different immune response 15 .…”

supporting

confidence: 91%

Anti-Taenia solium metacestode IgG antibodies in serum samples from inhabitants of a central-western region of Brazil

Oliveira

Rodrigues

Barcelos

et al. 2006

Rev. Inst. Med. trop. S. Paulo

View full text Add to dashboard Cite

SUMMARYA total of 354 serum samples from inhabitants who frequent the Clinical Laboratory in Catalão, Goiás, in the central-western region of Brazil, were collected from June to August, 2002. The samples were evaluated by indirect immunofluorescence antibody tests and an enzyme linked immunosorbent assay in order to detect anti-Taenia solium metacestode IgG antibodies. Reactive and inconclusive samples were tested by Western blotting (WB). Considering WB as a confirmation, the frequency of antibodies in the serum samples of the above population was 11.3% (CI 5.09 -17.51). The immunodominant bands most frequently recognized in WB were 64-68 kDa (97.5%) and 47-52 kDa (80%). The percentage of seropositivity to cysticercosis was significantly higher for individuals residing in areas without sewage systems (p < 0.0001). In conclusion, the results indicate a probable endemic situation of cysticercosis in this population. These results reinforce the urgent need for control and prevention measures to be taken by the local public health services.

show abstract

“…In the non NC group we obtained positive results mainly with the Tso antigen. The specificity of the ELISA with Tcra-vf has been reported as 80% and 96% by BUENO et al 5 and BRAGAZZA et al 4 , respectively, while PERALTA et al 17 reported 98% specificity for ELISA with 14 kDa glycoprotein of T. crassiceps. In this study the Tcra-gp antigen showed similar sensitivity and better specificity than that of Tcra-vf, so Tcra-gp seems to be the best choice to be attempt in order to improve the specificity of ELISA for screening purpose and efforts are underway through purification of the specific and immunodominant antigens.…”

Section: Discussionmentioning

confidence: 97%

Serodiagnosis of neurocysticercosis in patients with epileptic seizure using ELISA and immunoblot assay

Ishida

Peralta

Livramento

et al. 2006

Rev. Inst. Med. trop. S. Paulo

Self Cite

View full text Add to dashboard Cite

SUMMARYSera from 88 patients from Santa Catarina and São Paulo states of Brazil, with epileptic seizures who underwent cerebral computed tomography (CT) were analyzed for the detection of antibodies to T. solium cysticercus by ELISA and Immunoblot (IB) with the following antigens: Taenia solium cysticercus total saline (Tso), Taenia crassiceps cysticercus vesicular fluid (Tcra-vf) and T. crassiceps cysticercus glycoproteins (Tcra-gp). ELISA carried out with Tso, Tcra-vf and Tcra-gp antigens showed 95%, 90% and 80% sensitivities, respectively, and 68%, 85% and 93% specificities, respectively. In the epileptic patients group, ELISA positivity was 30%, 51% and 35% with Tso, Tcra-vf and Tcra-gp antigens respectively. Considering the IB as the confirmatory test, the positivity was 16% (14/88) in the epileptic patients total group and 22% (12/54) in the epileptic patients with positive CT and signals of cysticercosis. We found a significant statistical correlation among ELISA or IB results and the phase of the disease when any antigens were used (p < 0.05). We emphasize the need to introduce in the laboratory routine the search for neurocysticercosis (NC) in patients presenting with epileptic seizures because of the high risk of acquiring NC in our region and its potential cause of epilepsy.

show abstract

Frequency of serum anti-cysticercus antibodies in the population of a rural Brazilian community (Cássia dos Coqueiros, SP) determined by ELISA and immunoblotting using Taenia crassiceps antigens

Cited by 26 publications

References 24 publications

Taeniosis-cysticercosis complex in individuals of a peasants' settlement (Teodoro Sampaio, Pontal of Paranapanema, SP, Brazil)

Taeniosis-cysticercosis complex in individuals of a peasants' settlement (Teodoro Sampaio, Pontal of Paranapanema, SP, Brazil)

Anti-Taenia solium metacestode IgG antibodies in serum samples from inhabitants of a central-western region of Brazil

Serodiagnosis of neurocysticercosis in patients with epileptic seizure using ELISA and immunoblot assay

Contact Info

Product

Resources

About