Serodiagnosis of Aspergillosis

Identification of a concanavalin A-binding antigen of the cell surface ofSporothrix schenckii

“…Fractions containing Con A-binding antigens are usually the most reactive antigens described for several fungi as Aspergillus Jionigatus [20], Paracoccidioides brasiliensis [21], Blastono, ces dermatitidis [22] and also for S. schenckii extracellular antigens [9]. Similarly, in our study the Con A-binding fraction was more reactive with anti-S. schenckii rabbit serum.…”

Clinical manifestations and diagnosis of invasive aspergillosis in immunocompromised children

“…These crude extracts contain complex and undefined mixtures of antigenic polysaccharide, lipid and protein components [31,32,33,69]. Consequently, problems arise such as cross-reaction of antigenic determinants, lack of specificity and sensitivity, considerable batch to batch variations, qualitative and quantitative differences in the composition of antigens and contamination that occurs during the production process.…”

Use of Recombinant Mitogillin for Improved Serodiagnosis ofAspergillus fumigatus-Associated Diseases

“…Most immunoassays for detection of circulating antibodies are based on crude extracts of A. fumigatus. These extracts contain complex and undefined mixtures of polysaccharide and protein components and are derived from various sources such as conidial, mycelial, cytoplasmic, metabolic, or cell wall fractions of the fungus (13)(14)(15)28). Consequently, there is little knowledge about the role of the humoral immune response, the cross-reactivities of different epitopes in these crude antigenic preparations, the diagnostic value of class-specific antibody detection, and antibody kinetics in human aspergillosis (7).…”