Serodiagnosis as Adjunct Assay for Pertussis Infection in São Paulo, Brazil

Vaz-de-Lima, Lourdes R A; Martin, Monte D.; Pawloski, Lucia; Leite, Daniela; Rocha, Karen C. P.; Brito, Cyro Alves de; Vaz, Tânia; Martins, Luciano Moura; Alvarenga, Danielly P.; Ribeiro, Ana Freitas; Carvalhanas, Telma Regina Marques Pinto; Nakasaki, Rosa M. D.; Oliveira, Silvia Silva de; Waldman, Eliseu Alves; Tondella, M. Lucia

doi:10.1128/cvi.00760-13

Cited by 13 publications

(11 citation statements)

References 23 publications

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“…Despite this limitation, serology provides an additional option for patients presenting to the physician late in their illness. Similarly, data from IgG-PT ELISAs used for routine diagnosis in Massachusetts and São Paulo, Brazil have demonstrated that the inclusion of serology provides a more accurate estimate of the true burden of disease by identifying adolescent and adult pertussis infections that were missed by culture and PCR [ 3 , 26 , 27 ].…”

Section: Discussionmentioning

confidence: 99%

Clinical evaluation and validation of laboratory methods for the diagnosis of Bordetella pertussis infection: Culture, polymerase chain reaction (PCR) and anti-pertussis toxin IgG serology (IgG-PT)

et al. 2018

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IntroductionThe appropriate use of clinically accurate diagnostic tests is essential for the detection of pertussis, a poorly controlled vaccine-preventable disease. The purpose of this study was to estimate the sensitivity and specificity of different diagnostic criteria including culture, multi-target polymerase chain reaction (PCR), anti-pertussis toxin IgG (IgG-PT) serology, and the use of a clinical case definition. An additional objective was to describe the optimal timing of specimen collection for the various tests.MethodsClinical specimens were collected from patients with cough illness at seven locations across the United States between 2007 and 2011. Nasopharyngeal and blood specimens were collected from each patient during the enrollment visit. Patients who had been coughing for ≤ 2 weeks were asked to return in 2–4 weeks for collection of a second, convalescent blood specimen. Sensitivity and specificity of each diagnostic test were estimated using three methods—pertussis culture as the “gold standard,” composite reference standard analysis (CRS), and latent class analysis (LCA).ResultsOverall, 868 patients were enrolled and 13.6% were B. pertussis positive by at least one diagnostic test. In a sample of 545 participants with non-missing data on all four diagnostic criteria, culture was 64.0% sensitive, PCR was 90.6% sensitive, and both were 100% specific by LCA. CRS and LCA methods increased the sensitivity estimates for convalescent serology and the clinical case definition over the culture-based estimates. Culture and PCR were most sensitive when performed during the first two weeks of cough; serology was optimally sensitive after the second week of cough.ConclusionsTiming of specimen collection in relation to onset of illness should be considered when ordering diagnostic tests for pertussis. Consideration should be given to including IgG-PT serology as a confirmatory test in the Council of State and Territorial Epidemiologists (CSTE) case definition for pertussis.

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Section: Discussionmentioning

confidence: 99%

Clinical evaluation and validation of laboratory methods for the diagnosis of Bordetella pertussis infection: Culture, polymerase chain reaction (PCR) and anti-pertussis toxin IgG serology (IgG-PT)

et al. 2018

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“…Typical symptoms of pertussis, such as paroxysmal cough, vomiting after coughing, and inspiratory wheezing, are of limited value in the clinical diagnosis of pertussis (197,198) (2A). Antipertussis immunoglobulin G (anti-PT-IgG), polymerase chain reaction (PCR), and bacterial culture are helpful in the diagnosis of pertussis (199)(200)(201)(202)(203)(204)…”

Section: (2b)mentioning

confidence: 99%

Clinical Practice Guidelines for Diagnosis and Management of Cough—Chinese Thoracic Society (CTS) Asthma Consortium

Lai

Shen

Zhou³

et al. 2018

J. Thorac. Dis

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of cough was added; (V) the etiology and management of chronic cough in children was introduced; (VI) a section on uncommon causes of chronic cough; and (VII) added unexplained cough [refractory cough, cough hypersensitivity syndrome (CHS)]. Introduction of methodology (I) The target population: patients with cough. (II) The target users: respiratory specialists from all levels of hospitals, physicians of internal medicine and TCM, general practitioners, pediatricians, and other health-care providers.

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“…Many LMICs only provide wP vaccines. 26,[46][47][48][49] In the majority of countries where wP vaccines are used, 3 primary doses are administered at various intervals within the first year of life, followed by one or 2 boosters between 15 months and 5 y of age. Two primary immunization schedules are most commonly used.…”

Section: Pertussis Vaccinesmentioning

confidence: 99%

Immune persistence after pertussis vaccination

Chen

2017

Human Vaccines & Immunotherapeutics

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Pertussis is one of the most prevalent vaccine-preventable diseases worldwide. The true infection rate is significantly higher than the reported incidence rate. An increased prevalence of pertussis in older populations has been found, mainly caused by waning immunity after vaccination. Vaccine-induced immunity differs due to variation in vaccine content, schedule and coverage. Protection following acellular pertussis vaccines has been suggested to wane faster than whole cell pertussis vaccines. However, long-term immune persistence of whole cell pertussis vaccines may be confounded by a progressive acquisition of natural immunity. The World Health Organization has recommended that a switch from whole cell to acellular pertussis vaccines for primary immunization in infants should only be considered if additional periodic boosters or maternal immunization can be ensured and sustained in the national immunization schedules. In this review, we present data on immune persistence after different pertussis vaccinations and compare the findings from countries with different vaccination strategies. Future aspects in serological studies are briefly discussed.

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Serodiagnosis as Adjunct Assay for Pertussis Infection in São Paulo, Brazil

Cited by 13 publications

References 23 publications

Clinical evaluation and validation of laboratory methods for the diagnosis of Bordetella pertussis infection: Culture, polymerase chain reaction (PCR) and anti-pertussis toxin IgG serology (IgG-PT)

Clinical evaluation and validation of laboratory methods for the diagnosis of Bordetella pertussis infection: Culture, polymerase chain reaction (PCR) and anti-pertussis toxin IgG serology (IgG-PT)

Clinical Practice Guidelines for Diagnosis and Management of Cough—Chinese Thoracic Society (CTS) Asthma Consortium

Immune persistence after pertussis vaccination

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