Sero-epidemiological study of Taenia saginata cysticercosis in Belgian cattle

Dorny, Pierre; Vercammen, Francis; Brandt, Jef; Vansteenkiste, W; Berkvens, Dirk; Geerts, S.

doi:10.1016/s0304-4017(99)00196-x

Cited by 161 publications

(146 citation statements)

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“…The TCA treatment has been found to dissociate immuno-complexes in a number of studies (De Jonge et al 1987, Draelants et al 1995, Dorny et al 2000, Nguekam et al 2003.…”

Section: Discussionmentioning

confidence: 99%

“…Similar results were reported by Nguekam et al (2003), who found that the same happens with animals harboring small number of viable cysts (<100). The sensitivity of the test drops from 92.3% to 12.8% when less than 50 cysticerci are present (Van Kerchoven et al 1998, Dorny et al, 2000. According to Brandt et al (1992), differences in the sensitivity of the test may be related to the permeability of the host capsule around the cysticercus, which inϐluences the amount of excretory-secretory products released into the circulation.…”

Section: Discussionmentioning

confidence: 99%

“…The Ag-ELISA was performed as described by Dorny et al (2002), with minor modiϐications. Serum samples were pre--tre ated with 5% trich loroacetic acid (Dorny et al 2000, Nguekam et al 2003 and incubated for 20 min at room temperature to dissociate immune-complexes. After 5 minutes of centrifugation (10000g), the supernatant was neutralized using the same volume of a 0.6 M sodium carbonate/ bicarbonate buffer (pH 9.6).…”

Section: Methodsmentioning

confidence: 99%

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Cysticercosis in experimentally and naturally infected pigs: parasitological and immunological diagnosis

et al. 2012

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Our objective was to evaluate the diagnosis of swine cysticercosis by examining "ante mortem" (inspection of the tongue), "post mortem" (inspection and detailed necropsy) and ELISA for research in serum of antibodies (Ab-ELISA) and antigens (Ag-ELISA). Seven (7) pigs were experimentally infected orally with eggs of Taenia solium and another 10 were naturally infected. In the pigs experimentally infected, inspection of the tongue was negative in all animals, in the routine inspection detailed necropsy and cysticercis were identified in all of them. In pigs with heavy natural infection, inspection of the tongue identified cysticerci in two (20%), while at inspection with necropsy the parasites were identified in large quantities in all animals. In ELISA for antibody search (Ab-ELISA) TS-14 recombinant protein was used, and in search for antigen (Ag-ELISA) a monoclonal antibody against this protein. In animals experimentally infected, blood was collected weekly for 140 days. The Ab-ELISA identified an increase in titers of antibody to cysticerci 21 days after infection, and at the end of the experimental period six animals (86%) were positive to the test. The search for circulating antigens (Ag-ELISA) was positive in two pigs 28 to 91 days after infection. All naturally infected pigs were positive for Ag-ELISA and Ab-ELISA. The search for antibodies and antigens by ELISA in serum from 30 pigs of a local farm and without history of cysticercosis was negative. Thus, the use of TS-14 antigen in ELISA test (Ab-ELISA) can be useful for the diagnosis of cysticercosis in pigs with low infection.

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“…The TCA treatment has been found to dissociate immuno-complexes in a number of studies (De Jonge et al 1987, Draelants et al 1995, Dorny et al 2000, Nguekam et al 2003.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Cysticercosis in experimentally and naturally infected pigs: parasitological and immunological diagnosis

et al. 2012

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“…Sera from humans were tested in an Ag-ELISA (Brandt et al 1992) with the modifications, including pre-treatment of the sera by trichloroacetic acid and the interpretation of the results, as described by Dorny et al (2000) and Erhart et al (2002). Because a preliminary experiment, consisting in the dissection of the entire carcass of five pigs that were found positive in the Ag-ELISA, revealed that two pigs were infected with metacestodes of T. solium, another two with T. hydatigena and one pig with both parasites, it was decided not to use this test for serodiagnosis.…”

Section: Methodsmentioning

confidence: 99%

Taeniasis-cysticercosis in Southern Ecuador: assessment of infection status using multiple laboratory diagnostic tools

Rodríguez‐Hidalgo

Benítez‐Ortiz

Praet

et al. 2006

Mem. Inst. Oswaldo Cruz

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“…The Ag-ELISA as described by Dorny et al (2000) was used in this survey. Briefly, polystyrene ELISA plates were coated with a monoclonal Ab (B158C 11 A 10 ) and blocked with 1% heat inactivated newborn calf serum in PBS with 0.05% Tween 20 (0.05% PBS-Tw20).…”

Section: Circulating Antigen Detecting Elisa (Ag-elisa)mentioning

confidence: 99%

Combined use of an antigen and antibody detection enzyme‐linked immunosorbent assay for cysticercosis as tools in an epidemiological study of epilepsy in Burundi

Prado-Jean

Kanobana

Druet‐Cabanac

et al. 2007

Tropical Med Int Health

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Summaryobjective To evaluate the benefits of the detection of both circulating antibodies (Ab) and antigens (Ag) for the diagnosis of cysticercosis in people with epilepsy. Neurocysticercosis is a cause of neurological diseases world-wide, especially epilepsy. The clinical symptoms of neurocysticercosis are non-specific and diagnosis is often difficult.methods Serum samples were collected from subjects in a matched case-control study for epilepsy in the Kiremba area, Burundi, between March and April 2001 (epileptic cases ¼ 303; controls without epilepsy ¼ 606). The enzyme-linked immunosorbent assay (ELISA) was used for the detection of antibodies (Ab-ELISA) and circulating Ag (Ag-ELISA).results The Ab-ELISA revealed 58.7% positivity in epilepsy cases and 31.4% in healthy controls; and Ag-ELISA revealed 38.3% positivity in epilepsy cases and 20.0% in controls. The matched odds ratios were 3.6 (95%CI: 2.5-4.9) for Ab-ELISA, and 2.9 (95%CI: 2.1-4.3) for Ag-ELISA.conclusion Both Ag-and Ab-ELISA detected a significantly higher number of seropositives among people with epilepsy than among controls. The risk of epilepsy was high in cases with a positive Ag-ELISA, although less important than in cases with positivity for Ab-ELISA. Dead or degenerating cysticerci appear to be more frequently associated with epilepsy than living cysts. The high number of people with circulating Ag of Taenia solium suggests that the study area is a focus of active transmission of the parasite.

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Sero-epidemiological study of Taenia saginata cysticercosis in Belgian cattle

Cited by 161 publications

References 14 publications

Cysticercosis in experimentally and naturally infected pigs: parasitological and immunological diagnosis

Cysticercosis in experimentally and naturally infected pigs: parasitological and immunological diagnosis

Taeniasis-cysticercosis in Southern Ecuador: assessment of infection status using multiple laboratory diagnostic tools

Combined use of an antigen and antibody detection enzyme‐linked immunosorbent assay for cysticercosis as tools in an epidemiological study of epilepsy in Burundi

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