Serine Protease Inhibitor A3K Protects Rabbit Corneal Endothelium From Barrier Function Disruption Induced by TNF-α

Hu, Jiaoyue; Zhang, Zhenhao; Xie, Haipeng; Chen, Lelei; Yan, Zhou; Chen, Wensheng; Liu, Zuguo

doi:10.1167/iovs.12-10145

Cited by 18 publications

(17 citation statements)

References 39 publications

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“…DNAJB13 is a member of the heat shock protein (HSP) family, and has recently been associated with defects in cell motility (El Khouri et al, 2016 ). Serine protease inhibitor A3 is likely involved in controlling elastase activity and members of this family have been implicated in maintaining barrier functions (Hu et al, 2013 ). Micro RNA 543 (mir543) has been associated with mesenchymal stem cell differentiation, and thus may be related to changes in the cellular differentiation pathways (Xu et al, 2017 ).…”

Section: Resultsmentioning

confidence: 99%

Deep Sequencing Transcriptome Analysis of Murine Wound Healing: Effects of a Multicomponent, Multitarget Natural Product Therapy-Tr14

Laurent

Seilheimer

Tackett

et al. 2017

Front. Mol. Biosci.

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Wound healing involves an orchestrated response that engages multiple processes, such as hemostasis, cellular migration, extracellular matrix synthesis, and in particular, inflammation. Using a murine model of cutaneous wound repair, the transcriptome was mapped from 12 h to 8 days post-injury, and in response to a multicomponent, multi-target natural product, Tr14. Using single-molecule RNA sequencing (RNA-seq), there were clear temporal changes in known transcripts related to wound healing pathways, and additional novel transcripts of both coding and non-coding genes. Tr14 treatment modulated >100 transcripts related to key wound repair pathways, such as response to wounding, wound contraction, and cytokine response. The results provide the most precise and comprehensive characterization to date of the transcriptome's response to skin damage, repair, and multicomponent natural product therapy. By understanding the wound repair process, and the effects of natural products, it should be possible to intervene more effectively in diseases involving aberrant repair.

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Section: Resultsmentioning

confidence: 99%

Deep Sequencing Transcriptome Analysis of Murine Wound Healing: Effects of a Multicomponent, Multitarget Natural Product Therapy-Tr14

Laurent

Seilheimer

Tackett

et al. 2017

Front. Mol. Biosci.

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“…[17][18][19]27 In ocular surface, we previously demonstrated the beneficial effects of SERPINA3K in corneal alkali burn, 20 the protection of corneal endothelium from barrier function disruption 28 and protection of corneal epithelium from oxidative stress. 29 In this study, we provided novel evidences that SERPINA3K ameliorated the severity of squamous metaplasia and suppressed the formation of dry eye in a murine model.…”

Section: Discussionmentioning

confidence: 99%

Serine Protease Inhibitor A3K Suppressed the Formation of Ocular Surface Squamous Metaplasia in a Mouse Model of Experimental Dry Eye

Zhong

Yan

Wang

et al. 2014

Invest. Ophthalmol. Vis. Sci.

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“…The permeability of the corneal endothelium was evaluated by measuring FITC-dextran accumulation in the corneal tissue 28 , 29 . Rats were anaesthetized with 10% chloral hydrate to establish the AOH model.…”

Section: Methodsmentioning

confidence: 99%

Acute ocular hypertension disrupts barrier integrity and pump function in rat corneal endothelial cells

Zhang

et al. 2017

Sci Rep

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Acute ocular hypertension (AOH) frequently compromises corneal endothelial cell (CEC) function in clinical practice. This type of stress induces corneal oedema and a decrease in the corneal endothelial cell density (ECD). The anterior chamber of the right eye of Sprague-Dawley rats was irrigated with Balanced Salt Solution (BSS) for two hours, and the left eye served as a control to determine the time-dependent effects of AOH on endothelial cell morphology and function. The average intraocular pressure (IOP) increased to 82.6 ± 2.3 mmHg (normal range: 10.2 ± 0.4 mmHg) during anterior irrigation. Very soon after initiating irrigation, corneal oedema became evident and the cornea exhibited a significant increase in permeability to FITC-dextran. The peripheral ECD was significantly reduced, and the morphology of CECs became irregular and multiform. The structures of the zonula occludens-1 (ZO-1) and F-actin were severely disrupted. In addtion, Na,K-ATPase exhibited a dispersed expression pattern. Two days after irrigation, obvious CEC proliferation was observed, the ECD recovered to a normal level, and F-actin was dispersed throughout the cytoplasm. Seven days later, the CEC structure and function were nearly normalized. Based on the results obtained using this model, an acute IOP crisis exerts transient deleterious effects on CEC structure and function in rats.

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Serine Protease Inhibitor A3K Protects Rabbit Corneal Endothelium From Barrier Function Disruption Induced by TNF-α

Abstract: Citation: Hu J, Zhang Z, Xie H, et al. Serine protease inhibitor A3K protects rabbit corneal endothelium from barrier function disruption induced by TNF-a. Invest Ophthalmol Vis Sci.

Cited by 18 publications

References 39 publications

Deep Sequencing Transcriptome Analysis of Murine Wound Healing: Effects of a Multicomponent, Multitarget Natural Product Therapy-Tr14

Deep Sequencing Transcriptome Analysis of Murine Wound Healing: Effects of a Multicomponent, Multitarget Natural Product Therapy-Tr14

Serine Protease Inhibitor A3K Suppressed the Formation of Ocular Surface Squamous Metaplasia in a Mouse Model of Experimental Dry Eye

Acute ocular hypertension disrupts barrier integrity and pump function in rat corneal endothelial cells

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