Serial Studies of Bone Marrow-Derived Fibroblastoid Colony-Forming Cells and Granulocyte/Macrophage Precursor Cells in Patients with Acute Leukemia

Abstract

Bone marrow-derived fibroblastoid colony-forming cells (CFU-F) and granulocyte/macrophage precursor cells (CFU-GM) were studied in patients with acute leukemia. The numbers of CFU-F and CFU-GM were significantly lower in patients with acute myelogenous leukemia (AML) and acute lymphocytic leukemia (ALL) at diagnosis than in normal subjects, although patients with AML had a very wide range of CFU-F colony-forming efficiency. However, a suppressive effect of leukemic cells on normal CFU-F colony formation was not observed. CFU-F and CFU-GM in patients with acute leukemia recovered to normal levels when complete remission (CR) was achieved and decreased again at relapse. Serial studies showed that the increase in CFU-F preceded the recovery of CFU-GM. In AML, furthermore, patients who achieved CR had a higher number of CFU-F than patients without CR, suggesting that the CFU-F level at diagnosis may contribute to the prediction of the likelihood of remission induction in patients with AML.

Suppression of Normal Hematopoiesis in Acute Leukemia: Effect of Leukemic Cells on Bone Marrow Stromal Cells and Hematopoietic Progenitor Cells.

“…These results are inconsistent with some of the previous studies which demonstrated no inhibitory but rather an enhancing effect on either CFU-C (7, 8) or CFU-F (20,23). Such a discrepancy maybe due to differences in the experimental system used, especially in the source of LC.…”

“…Further, flbroblastoid colony forming cells (CFU-F), assayed by in vitro culture of bone marrowcells, are regarded as precursors of stromal cells, mainly of fibroblasts (18). Several studies have shownthat the number ofCFU-Fin the bone marrowin acute leukemia is decreased in vivo and in vitro (6,(19)(20)(21)(22)(23)(24), although there is considerable variation among patients according to the subtype of leukemia, especially between patients with acute myelogenous leukemia (AML)and acute lymphoblastic leukemia (ALL) (22,23). The growth of CFU-Fhas been suggested to be suppressed by leukemic cells (LC) and leukemic cell-conditioned medium (LC-CM) (6, 19), although controversy still exists (20,23 …”

Factors affecting mesenchymal stromal cells yield from bone marrow aspiration

“…After MSC was removed from its untoward microenvironment and cultured separately in vitro, the growth rate of MSC derived from leukemia patients was similar with the healthy donors. Several groups demonstrated that the yield of MSC colonies in patients with acute leukemia was significantly lower than that of normal group [28][29][30] . However, they all evaluated the number of MSC by counting the MSC colony per million nucleated cells.…”

Granulocyte-Macrophage Colony-Forming-Unit Assay and Liquid Suspension Culture of Bone Marrow and Peripheral Blood in Newly Diagnosed Childhood Acute Lymphoblastic Leukemia

“…In ALL, BM granulocyte-macrophage colony and cluster formations were invariably reported to be markedly decreased or showing no growth [8, 9, 10, 11, 12, 13, 14, 15] and PB colony formations to be increased [9, 15, 22] or variable [12, 23]. There was generally a lack of correlation between the results of CFU-GM in BM and in PB [24].…”

“…Marrow cultures exhibited abnormal growth patterns ranging from no growth to excessive microcluster formation in acute myeloid leukemia. By contrast, the granulocyte-macrophage colony and cluster formations in marrow culture were invariably reported to exhibit no or minimal growth in acute lymphoblastic leukemia (ALL) [8, 9, 10, 11, 12, 13, 14, 15]. However, studies in ALL have been limited, especially with respect to the in vitro cell growth patterns in liquid suspension culture.…”