Factors affecting mesenchymal stromal cells yield from bone marrow aspiration

Abstract

Objective: This study was to investigate the variables in bone marrow harvesting procedure and individual donor factors which can potentially affect the yield of mesenchymal stromal cells (MSC).Methods: We determined the yield of MSC from bone marrow under different clinical conditions by comparing the MSC colony numbers from: (1) donors of different ages; (2) healthy donors and patients with leukemia; (3) bone marrow aspirated at different time points during marrow harvesting; (4) bone marrow harvested by different needles.Results: During the process of harvesting, the number of MSC significantly decreased with increase number of aspiration, from 675/ml at the initial decreased to 60/ml after 100 ml bone marrow aspirated, and 50/ml after 200 ml bone marrow aspirated. The number of MSC retrieved from leukemia patients (99/ml bone marrow) was significantly lower than that of healthy donors (708/ml bone marrow). However, there was no significant difference in growth rate. There was no significant age-related difference of MSC yielded from donors <55 years. And there was no significant difference in MSC number between the samples from single end-holed needle and those from multiple-side-hole needle.Conclusion: The optimal bone marrow samples for MSC collection should be obtained earlier in the process of harvesting procedure. Bone marrow from donors <55 years was equally good as MSC sources. The autologous MSC from leukemia patients can be utilized for in-vitro MSC expansion.

Oxidative status predicts quality in human mesenchymal stem cells

“…MSC can be isolated from diverse adult tissues, including bone marrow stroma [4]. The number of MSC in the bone marrow might be reduced by diseases such as osteoporosis [5], leukemia [6], or lung and breast cancers [7]. Aging has also been proposed as an underlying cause of MSC deficiency in the bone marrow; however, this is still a matter of debate.…”

“…Aging has also been proposed as an underlying cause of MSC deficiency in the bone marrow; however, this is still a matter of debate. While a decline in MSC number in bone marrow stroma has been linked to increased donor age in several papers [8, 9], others report the absence of any correlation between the two factors [6, 10]. The reasons for exhaustion of MSC in the bone marrow are still debated; however, oxidative stress has been shown to be one of the major factors influencing tissue aging [11, 12], and as such merits in-depth investigation.…”

Regenerative Engineering of Cartilage Using Adipose-Derived Stem Cells

“…However, autologous bone marrow procurement is not without its limitations; it is a painful and invasive procedure that is associated with significant morbidity [33]. Moreover, deriving MSCs from bone marrow yields low cell numbers upon processing (approximately 1 MSCs per 10 5 aspirated bone marrow cells) [34, 35]. Generously assuming, a concentration of 3×10 7 nucleated cells per mL of bone marrow aspirate [36] would result in approximately only 600 MSCs per mL of marrow.…”

“…As mentioned previously, approximately only 0.001 % of all bone marrow stromal cells aspirated have been shown to hold multipotent properties [34]. From a practical standpoint, this yields approximately 50–675 MSCs per mL of bone marrow aspirate [35]. In contrast, anywhere from 1×10 5–6 ASCs have been routinely obtained from 1 mL of lipoaspirate [39].…”

Cell therapy for bone fracture repair: A comparative preclinical review of mesenchymal stromal cells from bone marrow and from adipose tissue

“…Alternative harvesting sites are femoral heads (from total hip arthroplasty of an elderly patient population), sternum and less frequently vertebrae. The commonly applied preparation method for the generation of BM-MSC is density gradient centrifugation, but other methods rely on their properties to adhere to plastic [1,[61][62][63][64][65][66] . Ad-MSC are isolated from lipoaspirates obtained during liposuction, lipoplasty or lipectomy procedures.…”