Serial studies of autologous antibody reactivity to squamous cell carcinoma of the head and neck

Vlock, Daniel R.; Arnold, Beth; Humpierres, J.; Schwartz, Donald R.; Baker, Shan R.; Krause, Charles J.; Swanson, Neil A.; Carey, Thomas E.

doi:10.1007/bf01741554

Cited by 7 publications

(11 citation statements)

References 16 publications

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“…The presence of circulating antigen in patients and the resulting formation of immune complexes may explain this. We have demonstrated that dissociation of immune complexes by acidification and ultrafiltration of sera augments autologous antibody reactivity in the majority of cases studied [9,11]. These findings support the hypothesis that autologous antibody to SCCHN and melanoma may be obscured by circulating antigen and that immune complexes may provide a readily accessible source of relevant antigen and antibody.…”

Section: Introductionsupporting

confidence: 68%

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Isolation and purification of a squamous cell carcinoma of the head and neck-associated antigen identified by autologous antibody

Vlock

Toporowicz

Arnold

et al. 1993

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

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We have previously shown that detection of autologous antibody activity to squamous cell carcinoma of the head and neck may be augmented by dissociation of immune complexes. Western blot analysis with autologous antibody has identified a 60 kDa squamous cell carcinoma of the head and neck-associated antigen in spent media and immune complex-dissociated serum ultrafiltrate not recognized by normal human sera. Antigen-containing fractions of spent media were eluted from anion exchange columns immediately after serum albumin indicating that the antigen has an acidic pI < 4. Preparative purification of the squamous cell carcinoma antigen was accomplished by anion exchange of concentrated spent media (protein concentration 300 mg/ml) followed by lectin affinity chromatography with a Triticum vulgaris column. A single 60 kDa band was detected by silver stain and Western blot in antigen-containing fractions eluted following lectin affinity chromatography and SDS-PAGE. Final concentration of the antigen was determined to be 1 microgram/ml of protein with relative activity increased 1600 x over unfractionated spent media. We conclude that a squamous cell carcinoma of the head and neck-associated antigen, detected by autologous antibody, is an acidic 60 kDa glycoprotein.

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Section: Introductionsupporting

confidence: 68%

“…Previous studies have noted autologous antibody reactivity against SCCHN in 24 of 41 systems tested with a median titer of 1:4 [9,10]. In the majority of cases, autologous antibody reactivity could only be detected in undiluted serum precluding further analysis.…”

Section: Introductionmentioning

confidence: 92%

Isolation and purification of a squamous cell carcinoma of the head and neck-associated antigen identified by autologous antibody

Vlock

Toporowicz

Arnold

et al. 1993

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

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“…When performing this analysis, no loss of prognostic information occurred. Other serum proteins, e.g., cytokine receptors 22 or autoantibodies, 23 possibly relate to the observed differences of T‐lymphocyte function dependent on prognosis 24 and could explain the observed differences.…”

Section: Discussionmentioning

confidence: 99%

Peripheral Blood T‐Lymphocyte and Monocyte Function and Survival in Patients With Head and Neck Carcinoma

2000

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Objectives/Hypothesis: To determine if the Tlymphocyte and monocyte functions of peripheral blood mononuclear cells (PBMCs) from patients with head and neck squamous cell carcinomas (HNSCC) are predictive factors for outcome. Study Design: A prospective study describing the outcome, as to total survival and death by disease after at least 40 months observation, of 81 previously untreated male HNSCC patients in relation to PBMC T-lymphocyte and monocyte function. Methods: T-lymphocyte mitogenesis and the cytokine level in culture supernatants of PBMC as well as monocytes were analyzed. These parameters were related to survival by Cox regression and Kaplan-Meier survival analysis. Results: When patients with high versus low T-lymphocyte mitogen-stimulated proliferations were compared, a decreased proliferation was seen to be related to worse outcome. The predictive value of T-lymphocyte proliferation was shown to be an independent prognostic factor when adjusted for stage and stratified for anatomic location in survival analysis. The predictive value was also retained when the serum values of the major serum proteins and hormones and scores based on the smoking and alcohol history were added to the survival analysis with lymphocyte proliferation. Supernatant levels of ␥-interferon, interleukin (IL)-2, or IL-4 in PBMC cultures were not related to outcome. Monocyte function measured by endotoxinstimulated IL-1␤, IL-6, IL-12, and tumor necrosis factor-␣ secretion did not relate to outcome of the patients. Conclusion: The PBMC T-lymphocyte-stimulated proliferation is an independent prognostic factor for male HNSCC patients.

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“…The IgA-anti-Fab autoantibodies described herein may be the result of sequence homologies between human Fab molecules and EBV or other viruses. Anti-Fab autoantibodies might react with protective anti-tumor (Vlock and Kirkwood, 1985;Vlock et al, 1992) or antiviral antibodies and, by exerting anti-idiotypic blocking activity, contribute to tumor progression. The elimination of IgA-anti-Fab autoantibodies from the patient's circulation might be a useful approach in the therapy of head-and-neck cancer.…”

Section: Discussionmentioning

confidence: 99%

Association of IgA‐anti‐Fab autoantibodies with disease stage in head‐and‐neck cancer

Süsal

Maier

Lorenz

et al. 1994

Intl Journal of Cancer

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Patients with head-and-neck cancer commonly have immune defects. It was reported that these patients have raised serum IgA levels. We investigated whether IgA-anti-Fab autoantibodies, which occur in association with immune dysfunction, are present in patients with head-and-neck cancer. Sera of 101 patients with squamous-cell carcinoma (SCCHN) and 8 patients with adenoid cystic carcinoma (ACCHN) of the head and neck were tested in ELISA for IgA-anti-Fab autoantibody activity. IgA-anti-Fab serum activity was significantly higher in both SCCHN and ACCHN patients than in healthy controls. In patients with SCCHN, an association between disease stage and IgA-anti-Fab activity was established. Stage-IV patients had significantly higher IgA-anti-Fab than stage-I patients or healthy controls. Stage-II and stage-III patients had intermediate levels. Extremely high IgA-anti-Fab activity was observed in 7 patients who died within 6 months following testing, suggesting a relationship of autoimmunity with terminal disintegration of physiological body functions. IgA-anti-Fab autoantibodies may explain the occurrence of immune defects in patients with head-and-neck cancer.

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Serial studies of autologous antibody reactivity to squamous cell carcinoma of the head and neck

Cited by 7 publications

References 16 publications

Isolation and purification of a squamous cell carcinoma of the head and neck-associated antigen identified by autologous antibody

Isolation and purification of a squamous cell carcinoma of the head and neck-associated antigen identified by autologous antibody

Peripheral Blood T‐Lymphocyte and Monocyte Function and Survival in Patients With Head and Neck Carcinoma

Association of IgA‐anti‐Fab autoantibodies with disease stage in head‐and‐neck cancer

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