Serial Propagation of Influenza B (Lee) Virus in a Transmissible Line of Canine Kidney Cells

Green, Irving J.

doi:10.1126/science.138.3536.42

Cited by 27 publications

(17 citation statements)

References 7 publications

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“…The allantoic sacs of embryonated eggs became an ideal substrate for influenza vaccines because they supported high antigen yields. The vast majority of current influenza vaccines are also produced in eggs from viruses propagated exclusively in eggs because vaccines produced in cell culture systems have not yet been licensed in most countries (22,33,50). Although the egg allantoic sac has been used successfully to produce influenza vaccines for 60 years, recent human H3N2 viruses have largely lost the ability to replicate in this host unless they acquire certain mutations in the HA (48,80).…”

Section: Discussionmentioning

confidence: 99%

Receptor Specificity of Influenza A H3N2 Viruses Isolated in Mammalian Cells and Embryonated Chicken Eggs

Stevens

Chen

Carney

et al. 2010

J Virol

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Isolation of human subtype H3N2 influenza viruses in embryonated chicken eggs yields viruses with amino acid substitutions in the hemagglutinin (HA) that often affect binding to sialic acid receptors. We used a glycan array approach to analyze the repertoire of sialylated glycans recognized by viruses from the same clinical specimen isolated in eggs or cell cultures. The binding profiles of whole virions to 85 sialoglycans on the microarray allowed the categorization of cell isolates into two groups. Group 1 cell isolates displayed binding to a restricted set of ␣2-6 and ␣2-3 sialoglycans, whereas group 2 cell isolates revealed receptor specificity broader than that of their egg counterparts. Egg isolates from group 1 showed binding specificities similar to those of cell isolates, whereas group 2 egg isolates showed a significantly reduced binding to ␣2-6-and ␣2-3-type receptors but retained substantial binding to specific O-and N-linked ␣2-3 glycans, including ␣2-3GalNAc and fucosylated ␣2-3 glycans (including sialyl Lewis x), both of which may be important receptors for H3N2 virus replication in eggs. These results revealed an unexpected diversity in receptor binding specificities among recent H3N2 viruses, with distinct patterns of amino acid substitution in the HA occurring upon isolation and/or propagation in eggs. These findings also suggest that clinical specimens containing viruses with group 1-like receptor binding profiles would be less prone to undergoing receptor binding or antigenic changes upon isolation in eggs. Screening cell isolates for appropriate receptor binding properties might help focus efforts to isolate the most suitable viruses in eggs for production of antigenically well-matched influenza vaccines.

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Section: Discussionmentioning

confidence: 99%

Receptor Specificity of Influenza A H3N2 Viruses Isolated in Mammalian Cells and Embryonated Chicken Eggs

Stevens

Chen

Carney

et al. 2010

J Virol

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“…They also derived the MDCK cell line from a seemingly normal adult female cocker spaniel but it appears they did not publish isolation of this line. However, MDCK cells were used to study viral infection [17], and subsequently characterised for the first time by Gaush et al in 1966 [1]. Then in the late 1970s/early 1980s the cell line started to become widely used for studying epithelial development and function [18].…”

Section: The Parental Mdck (Nbl-2) Linementioning

confidence: 99%

The MDCK variety pack: choosing the right strain

2011

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The MDCK cell line provides a tractable model for studying protein trafficking, polarity and junctions (tight, adherens, desmosome and gap) in epithelial cells. However, there are many different strains of MDCK cells available, including the parental line, MDCK I, MDCK II, MDCK.1, MDCK.2, superdome and supertube, making it difficult for new researchers to decide which strain to use. Furthermore, there is often inadequate reporting of strain types and where cells were obtained from in the literature. This review aims to provide new researchers with a guide to the different MDCK strains and a directory of where they can be obtained. We also hope to encourage experienced researchers to report the stain and origin of their MDCK cells.

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“…MDCK cells are readily permissive for a wide variety of flu strains [10,11] and lately optimal conditions for growing an influenza vaccine in MDCK cell line cultivated in a serum-free medium were determined [7]. Comparison of egg-to MDCK-grown influenza vaccine showed that the latter induced superior protection of ferrets against subsequent challenge with infectious virus grown in either host cell type [6].…”

Section: Page | 24mentioning

confidence: 99%

“…The original MDCK cell line was established in 1958 by Madin & Darby [9] and a few years later it was recognized that MDCK cells are readily permissive for a wide variety of flu strains [10,11].…”

Section: Introductionmentioning

confidence: 99%