Sequestration analysis for RNA polymerase I transcription factors with various deletion and point mutations reveals different functional regions of the mouse rRNA gene promoter.

Nagamine, M; Kishimoto, T; Aono, J; Kato, Haruyasu; Kominami, Ryo; Muramatsu, Masami

doi:10.1128/mcb.7.4.1486

Cited by 14 publications

(15 citation statements)

References 20 publications

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“…lanes 9 and 12). Previous studies indicate that, although both of these mutations reduce transcription, only the -16 mutant has lost the ability to compete with a wild-type promoter for the binding of mouse transcription factors (Nagamine et al 1987). These results suggest that the binding of mSL1 to the core promoter element plays an important role during mouse rRNA promoter recognition.…”

Section: Msl1 and M Ubf Form A Cooperative Dna-binding Complexsupporting

confidence: 47%

“…Bell, unpubl. ;Closet al 1986a, b;Nagamine et al 1987}. Thus, it is likely that mSL1 is an important component for TF-ID, TIF-IB, and factor D function.…”

Section: Role Of the Ubf-sl1 Complex In Promoter Recognitionmentioning

confidence: 99%

“…DNase I footprinting experiments with mSL1 map a protected region between -1 2 and -5 2 of the promoter {Fig. 3, lane 4), sequences previously shown to be important for the formation of stable transcription complexes at the mouse promoter (Clos et al 1986b;Tower et al 1986;Nagamine et al 1987). D N A binding experiments have also been performed by use of two point mutations at nucleotides -7 and -1 6 of the mouse core element {the generous gift of M. Muramatsu).…”

Section: Msl1 and M Ubf Form A Cooperative Dna-binding Complexmentioning

confidence: 99%

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Assembly of alternative multiprotein complexes directs rRNA promoter selectivity.

Bell¹,

Jantzen²,

Tjian³

1990

Genes Dev.

159

122

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How can trans-activators with the same DNA binding specificity direct different transcriptional programs? The rRNA transcriptional apparatus offers a useful model system to address this question and to dissect the mechanisms that generate alternative transcription complexes. Here, we compare the mouse and human transcription factors that govern species-specific RNA polymerase I promoter recognition. We find that both human and mouse rRNA transcription is mediated by a specific multiprotein complex. One component of this complex is the DNA-binding transcription factor, UBF. Paradoxically, human and mouse UBF display identical DNA binding specificities even though transcription of rRNA is species specific. Promoter selectivity is conferred by a second essential factor, SL1, which, for humans, does not bind DNA independently but, instead, cooperates with UBF in the formation of high-affinity DNA-binding complexes. In contrast, mouse SLI can selectively interact with DNA in the absence of UBF. Reconstituted transcription experiments establish that UBF and RNA polymerase I from the two species are functionally interchangeable, whereas mouse and human SL1 exhibit distinct DNA binding and transcription activities. Together, these results suggest a critical role for a specific multiprotein assembly in RNA polymerase I promoter recognition and reveal distinct mechanisms through which such complexes can generate functional diversity.[Key Words: RNA pol I; rRNA transcription; species specificity; transcription factors; UBF; SL1] Received February 7, 1990; revised version accepted March 14, 1990.Transcriptional initiation in eukaryotic cells is a highly regulated process requiring the correct association of numerous proteins into a specific complex with RNA polymerase {Mitchell and Tjian 1989; Saltzman and Weinmann 1989). Although mapping of essential promoter elements has identified multiple DNA sequences important for template recognition, how the different transcription factors work together with RNA polymerase to select these sequences as sites of initiation is poorly understood. Current results suggest strongly that the DNA sequence specificity of a single protein cannot account for promoter recognition by any of the three cellular RNA polymerases {Yoshinaga et al. 1987; Murphy et al. 1989; Smale and Baltimore 19891.Instead, it seems that the interactions of multiple proteins, both with the DNA and with one another, are required to generate the observed selectivity of initiation.The specificity of RNA polymerase I (RNA pol I} transcription is well suited for studies of promoter recognition. Unlike RNA pol II and RNA pol III, which recognize a wide variety of promoters, RNA pol I apparently initiates from only a single type of promoter in the cell, that of the large rRNA gene . This limited promoter range is reflected in the stringent species specificity of RNA pol I transcription.Studies using either intact cells or in vitro transcription extracts indicate that whereas very closely related species [e.g., mouse and rat)...

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Section: Msl1 and M Ubf Form A Cooperative Dna-binding Complexsupporting

confidence: 47%

“…Bell, unpubl. ;Closet al 1986a, b;Nagamine et al 1987}. Thus, it is likely that mSL1 is an important component for TF-ID, TIF-IB, and factor D function.…”

Section: Role Of the Ubf-sl1 Complex In Promoter Recognitionmentioning

confidence: 99%

Section: Msl1 and M Ubf Form A Cooperative Dna-binding Complexmentioning

confidence: 99%

See 1 more Smart Citation

Assembly of alternative multiprotein complexes directs rRNA promoter selectivity.

Bell¹,

Jantzen²,

Tjian³

1990

Genes Dev.

159

122

View full text Add to dashboard Cite

show abstract

“…This interaction is synergistic over an extended rDNA region, spanning the core promoter and an upstream control element (6, 34). It has also been shown that the core promoter sequence alone is sufficient to inhibit PolI transcription in trans, presumably competing for a factor(s) binding to it, and detection of such binding activity in cells supported this finding (23,30). A p70 protein that requires SL1 to bind the rRNA promoter has recently been isolated (47).…”

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confidence: 50%

Ribin, a Protein Encoded by a Message Complementary to rRNA, Modulates Ribosomal Transcription and Cell Proliferation

Kermekchiev¹,

Ivanova

2001

Molecular and Cellular Biology

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The control of rRNA transcription, tightly coupled to the cell cycle and growth state of the cell, is a key process for understanding the mechanisms that drive cell proliferation. Here we describe a novel protein, ribin, found in rodents, that binds to the rRNA promoter and stimulates its activity. The protein also interacts with the basal rRNA transcription factor UBF. The open reading frame encoding ribin is 96% complementary to a central region of the large rRNA. This demonstrates that ribosomal DNA-related sequences in higher eukaryotes can be expressed as protein-coding messages. Ribin contains two predicted nuclear localization sequence elements, and green fluorescent protein-ribin fusion proteins localize in the nucleus. Cell lines overexpressing ribin exhibit enhanced rRNA transcription and faster growth. Furthermore, these cells significantly overcome the suppression of rRNA synthesis caused by serum deprivation. On the other hand, the endogenous ribin level correlates positively with the amount of serum in the medium. The data show that ribin is a limiting stimulatory factor for rRNA synthesis in vivo and suggest its involvement in the pathway that adapts ribosomal transcription and cell proliferation to physiological changes.A major class of housekeeping genes, highly repeated and clustered in the nucleolus in eukaryotes, encode rRNA. The transcription of these genes, executed by RNA polymerase I (Pol I) and assisting factors, is strictly cell cycle and growth regulated (reviewed in references15, 32, and 34). It has been demonstrated that both activating factors and repressors are involved in a rapid adjustment of Pol I activity to the growth state of the cell (9,16,23,39). A Pol I-associated factor, TIF-IA/C*, implicated in the growth regulation of rRNA transcription in mammals, has recently been cloned (8). The activity of this factor fluctuates with the growth conditions, and its binding to the polymerase is required for a transcription-competent complex to form on the rRNA promoter (9, 39). Recent studies have shown that other Pol I transcription factors are also targets of regulation, finding that the phosphorylation status and activity of UBF, SL1, and TTFI are cell cycle controlled through cyclin-dependent kinases (20,24,41,44). Ribosomal transcription is a target of variety of factors affecting cell proliferation, such as hormones and phorbol esters, viral antigens, and the tumor suppressor proteins p53 and Rb (10,15,16,48,49).Pol I transcription machinery is generally species specific (15, 32). The structure divergence found in rRNA promoters of different species could partially account for this. SL1 has been shown to play a major role in selection of the rRNA promoter by homologous Pol I transcription apparatus, although UBF and Pol I can also contribute to this selectivity (5, 15, 37). The DNA-protein and protein-protein aspects of specific recognition of the rRNA promoter are not yet clear. Previous data demonstrated that UBF and SL1 can interact with the rRNA promoter. This interaction is...

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“…In this context, two observations appear to be relevant. First, both Muramatsu et al (50) and Sollner-Webb et al (51) reported that the 3Ј boundary of essential elements of the rDNA promoter extended to at least ϩ9. Second, we reported (52) that the sequences from ϩ2 to ϩ16 of the rat, mouse, and human rDNA promoters were identical.…”

Section: Discussionmentioning

confidence: 99%

DNA Binding by the Ribosomal DNA Transcription Factor Rrn3 Is Essential for Ribosomal DNA Transcription

Stepanchick

Zhi

Cavanaugh

et al. 2013

Journal of Biological Chemistry

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Background: Transcription initiation by RNA polymerase I requires protein-protein interactions between Rrn3, polymerase, and core factors. Results: Mutagenesis of a putative DNA binding domain in Rrn3 had no effect on essential protein-protein interactions, but abrogated DNA binding and inactivated Rrn3 function in transcription. Conclusion: DNA binding is essential for Rrn3 to function in transcription. Significance: DNA binding by Rrn3 may provide an additional target to regulate rDNA transcription.

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Sequestration analysis for RNA polymerase I transcription factors with various deletion and point mutations reveals different functional regions of the mouse rRNA gene promoter.

Cited by 14 publications

References 20 publications

Assembly of alternative multiprotein complexes directs rRNA promoter selectivity.

Assembly of alternative multiprotein complexes directs rRNA promoter selectivity.

Ribin, a Protein Encoded by a Message Complementary to rRNA, Modulates Ribosomal Transcription and Cell Proliferation

DNA Binding by the Ribosomal DNA Transcription Factor Rrn3 Is Essential for Ribosomal DNA Transcription

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