Abstract:Alfalfa (Medicago sativa) is the most important leguminous forage crop. Its yield and forage quality will be severely affected by the outbreak of defoliator insects, especially Spodoptera litura. Here, we found that the expression of alfalfa precursor miR396 genes (pre-MsmiR396a/b) was significantly down-regulated firstly and then up-regulated in wound treatment that simulating feeding injury of defoliator insects. To verify the function of microRNA396 (miR396) in insect resistance of alfalfa, we generated MIM… Show more
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