Sequential Syntheses of Various Molecular Species of Ribonucleic Acid in the Early Phase of Conidia Germination in Aspergillus Oryzae

Ono, Tetsu; Kimura, Kôichi; Yanagita, Tomomichi

doi:10.2323/jgam.12.13

Cited by 17 publications

(14 citation statements)

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“…During germination of Aspergillus oryzae conidia the synthesis of rRNA began immediately, followed by tRNA and, lastly, mRNA (Ono, Kimura & Yanagita, 1966;Tanaka, Ono & Yanagita, 1966). In both these fungi as well as in B. theobromae it was at least 60 min after the start of the germination process before the synthesis of all classes of RNA was detected.…”

mentioning

confidence: 99%

Synthesis of Ribonucleic Acids During the Germination of Botryodiplodia theobromae Pycnidiospores

Knight¹,

Etten²

1976

Journal of General Microbiology

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Botryodiplodia theobromae pycnidiospores, protein synthesis was initiated in the absence of detectable ribonucleic acid (RNA) synthesis. The lack of RNA synthesis during the early stages of germination was not an artefact due to reduced precursor permeability or to changes in the nucleotide pool. Further studies showed that the synthesis of messenger RNA and transfer RNA began about 30 min after the initiation of germination and that of 5s RNA after 45 min, whereas ribosomal RNA synthesis was not detected until after I h of germination. Approximately 45 to 55 % of the messenger-like RNA synthesized during the first hour of germination was devoid of polyadenylate segments.

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confidence: 99%

Synthesis of Ribonucleic Acids During the Germination of Botryodiplodia theobromae Pycnidiospores

Knight¹,

Etten²

1976

Journal of General Microbiology

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“…7) is more evidence that dormant spores contained messenger RNA. Data on other fungal spores support the generality of this hypothesis (Ono, Kimura & Yanagita, 1966;Staples, App, McCarthy & Gerosa, 1966;Ramakrishnan & Staples, 1968;Hollomon, 1969Hollomon, , 1970.…”

Section: W B a C O N And A S S U S S M A Nmentioning

confidence: 51%

Effects of the Self-Inhibitor of Dictyostelium discoideum on Spore Metabolism

Bacon

Sussman

1973

Journal of General Microbiology

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The effects of the self-inhibitor, N,N-dimethylguanosine, on the metabolism of spores of Dictyostelium discoideum were investigated. It is concluded that since both self-inhibited and activated spores had similar initial Qo, values, the site of action of the self-inhibitor was not on respiratory metabolism. Incorporation of [U14C]glucose revealed that organic acids and amino acids accumulated in self-inhibited spores and that protein synthesis was inhibited. Succinic acid accumulated only when the activated self-inhibited spores became anaerobic so its accumulation was not directly related to self-inhibition. Phenylalanine, methionine and tryptophan were absent in dormant spores but appeared after heatactivation and accumulated in self-inhibited spores. The accumulation of amino acids was related to the inability of self-inhibited spores to synthesize protein.In viva analysis showed that protein synthesis began early in spore germination and that the self-inhibitor immediately arrested this process when added to activated spores and slowed it when added to germinating spores. No effect of self-inhibitor on RNA synthesis during germination was detected. Thus, we conclude that the self-inhibitor, a methylated base of guanine, regulated spore germination by preventing the synthesis of protein necessary for subsequent development.

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“…Previous observations in this laboratory showed that 14C02 was incorporated into RNA in germinating A. oryzae conidia (12)(13)(14) and that the rate of incorporation into nucleic acid bases was the highest in purine bases, especially in adenine (15).…”

Section: Results Effect Of Adenine On Germinationmentioning

confidence: 99%

“…Samples of RNA for sedimentationn analysis by sucrose density-gradient centrifugation were prepared by the . sodium dodecylsulfate (SDS)-phenol method according to ONO et al (12). Labeled frozen conidia were resuspended in 1 ml of extraction medium consisting of buffer I (5 x 10-2M acetate buffer, pH 5.5, containing 10i3M MgCl2),.…”

Section: Cultivation Of Conidiamentioning

confidence: 99%

Transient Effect of Exogenous Adenine on Germination of Asrergillus Oryzae Conidia

Tsay

Hanaoka

1972

J. Gen. Appl. Microbiol.

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Aspergillus oryzae conidia germinate with good synchrony in a modified Czapek's medium supplemented with L-alanine and adenine. The present report deals with the role of adenine in the process of germination.Exogenous adenine is required only transiently during a period of 40-90 min after inoculation.14C-Adenine incorporation experiments showed that inosine, AMP, ATP, and NAD comprised nearly 90% of radioactivity in the acid-soluble fraction.Incorporation of 14C-adenine into RNA was accelerated markedly in this transient period. The rate of subsequent protein synthesis was retarded if adenine was not added during the transient period.The present data suggest that the exogenous adenine serves as one of the initiation factors for vegetative growth but not as a trigger for germination.

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Sequential Syntheses of Various Molecular Species of Ribonucleic Acid in the Early Phase of Conidia Germination in Aspergillus Oryzae

Cited by 17 publications

References 0 publications

Synthesis of Ribonucleic Acids During the Germination of Botryodiplodia theobromae Pycnidiospores

Synthesis of Ribonucleic Acids During the Germination of Botryodiplodia theobromae Pycnidiospores

Effects of the Self-Inhibitor of Dictyostelium discoideum on Spore Metabolism

Transient Effect of Exogenous Adenine on Germination of Asrergillus Oryzae Conidia

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