Synthesis of Ribonucleic Acids During the Germination of Botryodiplodia theobromae Pycnidiospores

Knight, Robert H.; Etten, James L. Van

doi:10.1099/00221287-95-2-257

Cited by 17 publications

(11 citation statements)

References 20 publications

(8 reference statements)

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“…What controls exist to prevent protein synthesis from occurring until germination is an open question. Our data, as well as the data of others (3,7,11,12,14,15,16,18,19,28,29,30,35), would argue that regulation must be at least partially at the level of translation. The observation that there is considerable poly(A)(+) RNA in yeast ascospores and that some of it is associated with polyribosomes suggests a number of molecular approaches to questions of maternal effects and to the persistence of information in RNA throughout the processes of meiosis and sporulation.…”

Section: J Bacteriolsupporting

confidence: 81%

Properties of polyadenylate-associated ribonucleic acid from Saccharomyces cerevisiae ascospores

Harper¹,

Clancy²,

Magee³

1980

J Bacteriol

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Bulk ribonucleic acid (RNA) was isolated from mechanically disrupted ascospores of Saccharomyces cerevisiae. After two passes over an oligo(dT1o) cellulose column, the portion which bound, called poly(A)(+)*, was characterized. It is heterodisperse in size with a mean molecular weight of approximately 4 x i05, but contains some species as large as 7 x 105. The base composition is similar to vegetative poly(A)(+) RNA. The polyadenylate segment is also heterogenous in size, ranging from 90 to 20 bases in length, with a peak at approximately 60 nucleotides in length. Pulse-labeling of asci with [3H-methyl]methionine yields two "caps," 7-methyl guanosine-5'-triphosphoryl-5'-adenosine (or guanosine), identical to that found in vegetative poly(A)(+) RNA. The poly(A)(+) RNA in spores is found in polyribosomes which are, on the average, smaller than vegetative ones. Long-term labeling studies indicate that the fraction of poly(A)(+) RNA in spores is similar to that in vegetative cells.

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Section: J Bacteriolsupporting

confidence: 81%

Properties of polyadenylate-associated ribonucleic acid from Saccharomyces cerevisiae ascospores

Harper¹,

Clancy²,

Magee³

1980

J Bacteriol

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“…A number of fungal species, such as Botryodiplodia theobromae (Knight et al ., 1976), Mucor racemosus (Linz et al ., 1982) and Neurospora tetrasperma (Plesofsky‐Vig et al ., 1992), are known to produce stored mRNA that is translated upon germination. Given the importance of Ptr ToxB in establishing a compatible interaction between host and pathogen, its presence in conidia presumably enables faster toxin production during the early stages of infection.…”

Section: Discussionmentioning

confidence: 99%

Quantification of ToxB gene expression and formation of appressoria by isolates of Pyrenophora tritici‐repentis differing in pathogenicity

et al. 2008

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Tan spot, caused by the fungus Pyrenophora tritici-repentis , is an important foliar disease of wheat. The pathogen produces at least three host-specific toxins, including Ptr ToxB, a 6·6 kDa protein that causes chlorosis in sensitive wheat genotypes and is encoded by the ToxB gene. Using quantitative reverse transcriptase (qRT)-PCR, transcript levels of ToxB homologues found in virulent ( ≡ pathogenic) and weakly virulent ( ≡ weakly pathogenic) race 5 isolates and an avirulent ( ≡ nonpathogenic) race 4 isolate of the fungus were compared in conidia, mycelia, and in host tissues after inoculation of resistant and susceptible wheat genotypes. Abundance of the ToxB transcript was greatest in the virulent isolate, followed by the weakly virulent and avirulent isolates, and was positively correlated with the development of chlorosis in the susceptible wheat genotype; transcript was detectable in mycelia and conidia of all isolates tested, and levels peaked at 24 h after inoculation of the race 5 isolates onto resistant and susceptible wheat hosts, before disease symptoms developed. Although all isolates could penetrate host epidermal cells to varying degrees, transcript abundance was positively correlated with the greater and more rapid development of appressoria. Therefore Ptr ToxB may have other roles in the basic pathogenic fitness of the fungus and/or in pre-penetration processes, suggesting that quantitative variation in the virulence of P. tritici-repentis is related to the extent of ToxB gene expression.

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“…The dormant spores of the fungus Botryodiplodia theobromae contain latent mRNA which is preserved during cell dormancy and which is translated immediately upon the initiation of spore germination in the absence of new mRNA synthesis (9,16). These early translational products have not been identified, and it would be of considerable interest to learn what genetic information is encoded within this mRNA and which of the proteins translated from this mRNA are essential for completion of spore germination.…”

mentioning

confidence: 99%

In Vitro Translation of Polyadenylate-Containing RNAs from Dormant and Germinating Spores of the Fungus Botryodiplodia theobromae

Wenzler

Brambl

1978

J Bacteriol

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Polyadenylated RNA isolated by oligodeoxythymidylate-cellulose chromatography from spores of the fungus Botryodiplodia theobromae was translated in a cell-free protein-synthesizing system derived from wheat embryo. Reaction conditions which would yield efficient and accurate in vitro translation of the spore RNA were established. Dual isotopically labeled mixtures of in vitro translation products from germinated and dornant spore polyadenylated RNA, as well as polyadenylated RNAs from intermediate stages of germination, produced qualitatively similar gel electrophoresis patterns, with polypeptides of 10,000 to 55,000 molecular weight. Proteins synthesized in vivo and extracted from germinating spores at three different stages possessed a greater size range, with molecular weights up to 85,000, although the in vitro synthesis apparently did yield the lower-molecular-weight proteins which were synthesized in vivo. Tryptic digest patterns of proteins translated in vitro from polyadenylated RNA of dormant and germinated spores were found to be identical in positions in only 40% of the spots. Furthermore, a dual-label comparison by isoelectric focusing of proteins translated from polyadenylated RNA of germinated and dormant spores also showed qualitative and quantitative differences among the in vitro translation products. We conclude that there are differences between the in vitro translation products of mRNA from dormant and germinated spores and that the mRNA preserved in the dormant spores contains genetic infornation which is qualitatively different from that of the germinated spores.on August 1, 2020 by guest

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Synthesis of Ribonucleic Acids During the Germination of Botryodiplodia theobromae Pycnidiospores

Cited by 17 publications

References 20 publications

Properties of polyadenylate-associated ribonucleic acid from Saccharomyces cerevisiae ascospores

Properties of polyadenylate-associated ribonucleic acid from Saccharomyces cerevisiae ascospores

Quantification of ToxB gene expression and formation of appressoria by isolates of Pyrenophora tritici‐repentis differing in pathogenicity

In Vitro Translation of Polyadenylate-Containing RNAs from Dormant and Germinating Spores of the Fungus Botryodiplodia theobromae

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