Sequential saccharification of corn fiber and ethanol production by the brown rot fungus Gloeophyllum trabeum

Rasmussen, Mary L.; Shrestha, Prachand; Khanal, Samir Kumar; Pometto, Anthony L.; Leeuwen, J. van

doi:10.1016/j.biortech.2009.12.115

Cited by 78 publications

(44 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Therefore, it is evident that both starch and hemi/cellulose fractions contributed significantly to enzyme induction and to saccharification and fermentation of corn fiber to ethanol. Similar results were also observed by Shrestha et al (5 ) and Rasmussen et al (6 ) for white-and brown-rot saccharification studies, respectively. There were no statistical differences between R-amylase and glucoamylase activities for all three fungal cultures ( Table 2, section b).…”

Section: Resultssupporting

confidence: 90%

Enzyme Production by Wood-Rot and Soft-Rot Fungi Cultivated on Corn Fiber Followed by Simultaneous Saccharification and Fermentation

Shrestha

Khanal

Pometto

et al. 2009

J. Agric. Food Chem.

View full text Add to dashboard Cite

This research aims at developing a biorefinery platform to convert lignocellulosic corn fiber into fermentable sugars at a moderate temperature (37 °C) with minimal use of chemicals. White-rot (Phanerochaete chrysosporium), brown-rot (Gloeophyllum trabeum), and soft-rot (Trichoderma reesei) fungi were used for in situ enzyme production to hydrolyze cellulosic and hemicellulosic components of corn fiber into fermentable sugars. Solid-substrate fermentation of corn fiber by either white-or brown-rot fungi followed by simultaneous saccharification and fermentation (SSF) with coculture of Saccharomyces cerevisiae has shown a possibility of enhancing wood rot saccharification of corn fiber for ethanol fermentation. The laboratory-scale fungal saccharification and fermentation process incorporated in situ cellulolytic enzyme induction, which enhanced overall enzymatic hydrolysis of hemi/cellulose components of corn fiber into simple sugars (mono-, di-, and trisaccharides). The yeast fermentation of the hydrolyzate yielded 7.8, 8.6, and 4.9 g ethanol per 100 g corn fiber when saccharified with the white-, brown-, and soft-rot fungi, respectively. The highest ethanol yield (8.6 g ethanol per 100 g initial corn fiber) is equivalent to 35% of the theoretical ethanol yield from starch and cellulose in corn fiber. This research has significant commercial potential to increase net ethanol production per bushel of corn through the utilization of corn fiber. There is also a great research opportunity to evaluate the remaining biomass residue (enriched with fungal protein) as animal feed. This research aims at developing a biorefinery platform to convert lignocellulosic corn fiber into fermentable sugars at a moderate temperature (37°C) with minimal use of chemicals. White-rot (Phanerochaete chrysosporium), brown-rot (Gloeophyllum trabeum), and soft-rot (Trichoderma reesei) fungi were used for in situ enzyme production to hydrolyze cellulosic and hemicellulosic components of corn fiber into fermentable sugars. Solid-substrate fermentation of corn fiber by either white-or brown-rot fungi followed by simultaneous saccharification and fermentation (SSF) with coculture of Saccharomyces cerevisiae has shown a possibility of enhancing wood rot saccharification of corn fiber for ethanol fermentation. The laboratory-scale fungal saccharification and fermentation process incorporated in situ cellulolytic enzyme induction, which enhanced overall enzymatic hydrolysis of hemi/cellulose components of corn fiber into simple sugars (mono-, di-, and trisaccharides). The yeast fermentation of the hydrolyzate yielded 7.8, 8.6, and 4.9 g ethanol per 100 g corn fiber when saccharified with the white-, brown-, and soft-rot fungi, respectively. The highest ethanol yield (8.6 g ethanol per 100 g initial corn fiber) is equivalent to 35% of the theoretical ethanol yield from starch and cellulose in corn fiber. This research has significant commercial potential to increase net ethanol production per bushel of corn through the utilization of corn...

show abstract

Section: Resultssupporting

confidence: 90%

Enzyme Production by Wood-Rot and Soft-Rot Fungi Cultivated on Corn Fiber Followed by Simultaneous Saccharification and Fermentation

Shrestha

Khanal

Pometto

et al. 2009

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…Take crops straw for example, it is not only valuable in improvement of soil properties and crop production (Shi et al, 2010;Tejada et al, 2008;Kasteel et al, 2007;Liu et al, 2006;Kumar and Goh, 2000), composted fertilizer (Lim et al, 2012), forage processing, biofuel production (Tian et al, 2013;Sun et al, 2010;Rasmussen et al, 2010;Han et al, 2009), and industrial utilization (Suzuki et al, 2007), but also in the research potential and utilizing prospects in the field of agricultural pests management (Cao et al, 2010;Han et al, 2010;Ma et al, 2013), environmental pollution abatement (Liu et al, 2012;Chen et al, 2008;Chen and Yuan, 2011;Jones et al, 2011), etc. (Duan et al, 2012Woolf et al, 2010).…”

Section: Agriculture Waste (Residue) Resources and Environmental Sustmentioning

confidence: 99%

Growth disturbance of extracts from several crops straw (residue) on Ageratina adenophora and biological-control implications in hazardous weed invasion for eco-restoration

Huang

et al. 2014

Ecological Engineering

View full text Add to dashboard Cite

a b s t r a c tLaboratory biological simulation experiment was conducted to investigate growth disturbance of high, moderate, low concentration of aqueous extracts (i.e. the original extracts with a solid-liquid ratio of 1:40 g mL −1 and its 5 times diluents and 25 times diluents) from several crops straw (residue) on Ageratina adenophora, a worldwide notorious invasive weed. The results showed: (a) aqueous extracts from several crops straw (residue) brought about different impacts on the single index for germination and growth of A. adenophora, e.g., high concentration of aqueous extracts from Brassica oleracea waste leaves showed a strong inhibition against the germination rate (GR) and germination index (GI) of A. adenophora, while high concentration of aqueous extracts from Vicia cracca straw showed a strong inhibition against radicle length (RL) and hypocotyl length (HL) of A. adenophora; (b) high concentration of aqueous extracts from B. oleracea waste leaves and high, moderate and low concentration of aqueous extracts from Oryza sativa straw and Triticum aestivum straw showed rather strong synthetic effects (inhibition) on GR and GI of A. adenophora, which could be chosen for the control over the seeds germination of A. adenophora; (c) high and moderate concentrations of aqueous extracts from V. cracca straw, high concentration of aqueous extracts from B. campestris waste leaves, and moderate and low concentrations of aqueous extracts from O. sativa straw and T. aestivum straw showed rather strong synthetic effects (inhibition) on RL and HL of A. adenophora, which could be selected as ideal materials for the control over the seedlings growth of A. adenophora; and (d) high concentrations of aqueous extracts from V. cracca straw, B. oleracea waste leaves and B. campestris waste leaves, and high, moderate and low concentrations of aqueous extracts from O. sativa straw and T. aestivum straw showed rather strong synthetic effects (inhibition) on GR, GI, RL and HL of A. adenophora, which could be selected as ideal materials for the control over the seeds germination and seedlings growth of A. adenophora. Thus, this study would provide a theoretic guidance and technical support for the resources utilization of crops straw (residue) and the prevention and control over invasive weeds as well.

show abstract

“…Two ml of fungal biomass [1.5% (w/v) P. chrysosporium and 1.0% (w/v) G. trabeum] in mineral salt solution was then added. The bottles were rolled on their sides and the marbles assisted in uniformly dispersing and coating the corn stover and fungi mixture along the inner surface [38,44]. Solid substrate fermentation was then performed for 4 days at 37 o C in a humidified incubator for in situ production of cellulases and hemicellulases prior to the addition of the ethanolic microorganism.…”

Section: Solid Substrate Fermentation For Enzyme Inductionmentioning

confidence: 99%

“…Unlike the SSF process in previous works, ours does not use pretreated corn stover samples [13,19,25,42] or the addition of expensive commercial enzymes [12,26,53]. Instead, cellulases and hemicellulases are produced by G. trabeum and P. chrysosporium in situ upon corn stover enzyme induction performed via solid substrate fermentation in a pH range of 4.5-4.8 at 37 o C for 4 days, conditions that are suitable not only for the growth of the fungi but also for production of cellulolytic enzymes [38,43,44]. As seen in Table 2, our assay using the NanoDrop 1000 spectrophotometer indicated that protein was produced during the induction stage, and production was higher in the stover and P. chrysosporium combination compared with the stover and G. trabeum combination, at 14.06 and 11.61 mg/ml, respectively.…”

Section: Enzyme Induction On Untreated Corn Stovermentioning

confidence: 99%

See 1 more Smart Citation

Simultaneous Saccharification and Fermentation of Ground Corn Stover for the Production of Fuel Ethanol Using Phanerochaete chrysosporium, Gloeophyllum trabeum, Saccharomyces cerevisiae, and Escherichia coli K011

Vincent¹

2011

J. Microbiol. Biotechnol.

View full text Add to dashboard Cite

Enzymatic saccharification of corn stover using Phanerochaete chrysosporium and Gloeophyllum trabeum and subsequent fermentation of the saccharification products to ethanol by Saccharomyces cerevisiae and Escherichia coli K011 were achieved. Prior to simultaneous saccharification and fermentation (SSF) for ethanol production, solid-state fermentation was performed for four days on ground corn stover using either P. chrysosporium or G. trabeum to induce in situ cellulase production. During SSF with S. cerevisiae or E. coli, ethanol production was the highest on day 4 for all samples. For corn stover treated with P. chrysosporium, the conversion to ethanol was 2.29 g/100 g corn stover with S. cerevisiae as the fermenting organism, whereas for the sample inoculated with E. coli K011, the ethanol production was 4.14 g/100 g corn stover. Corn stover treated with G. trabeum showed a conversion 1.90 and 4.79 g/100 g corn stover with S. cerevisiae and E. coli K011 as the fermenting organisms, respectively. Other fermentation co-products, such as acetic acid and lactic acid, were also monitored. Acetic acid production ranged between 0.45 and 0.78 g/100 g corn stover, while no lactic acid production was detected throughout the 5 days of SSF. The results of our experiment suggest that it is possible to perform SSF of corn stover using P. chrysosporium, G. trabeum, S. cerevisiae and E. coli K011 for the production of fuel ethanol.

show abstract

Sequential saccharification of corn fiber and ethanol production by the brown rot fungus Gloeophyllum trabeum

Cited by 78 publications

References 18 publications

Enzyme Production by Wood-Rot and Soft-Rot Fungi Cultivated on Corn Fiber Followed by Simultaneous Saccharification and Fermentation

Enzyme Production by Wood-Rot and Soft-Rot Fungi Cultivated on Corn Fiber Followed by Simultaneous Saccharification and Fermentation

Growth disturbance of extracts from several crops straw (residue) on Ageratina adenophora and biological-control implications in hazardous weed invasion for eco-restoration

Simultaneous Saccharification and Fermentation of Ground Corn Stover for the Production of Fuel Ethanol Using Phanerochaete chrysosporium, Gloeophyllum trabeum, Saccharomyces cerevisiae, and Escherichia coli K011

Contact Info

Product

Resources

About