Sequential determination of fat- and water-soluble vitamins in Rhodiola imbricata root from trans-Himalaya with rapid resolution liquid chromatography/tandem mass spectrometry

Tayade, Amol B.; Dhar, Priyanka; Kumar, Jatinder; Sharma, Manu; Chaurasia, O. P.; Srivastava, Roshni

doi:10.1016/j.aca.2013.05.062

Cited by 27 publications

(16 citation statements)

References 51 publications

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“…These were the prime raw ingredient of the phytococktail and may contribute to the total antioxidant capacity. In addition, apricot and roseroot also possess a diverse array of bioactive compounds [6,18-20] which could be responsible for the elevated antioxidant properties of the phytococktail extracts.…”

Section: Discussionmentioning

confidence: 99%

“…The advancement in the health promoting properties has led to use them as nutritional supplements [13-16]. The bioactive phytochemical components, medicinal values, therapeutic potential and nutritional properties of these three plants have been extensively studied by previous researchers [6,17-20] and the plant parts having medicinal properties were also reported to be safe and non-toxic [21-26]. We studied the methanol (hydrophilic) and n-hexane (lypophilic) extracts of the phytococktail to measure the total antioxidant capacity because both hydrophilic and lypophilic antioxidants contribute to the total antioxidant capacity.…”

Section: Introductionmentioning

confidence: 99%

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Chemical composition and antioxidant capacities of phytococktail extracts from trans-Himalayan cold desert

Dhar

Bajpai

Tayade

et al. 2013

BMC Complement Altern Med

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BackgroundHimalayan plants are widely used in traditional system of medicine both as prophylactics and therapeutics for high altitude maladies. Our aim was to evaluate the antioxidant capacities and bioactive compounds of methanol and n-hexane extracts of the phytococktail comprising of sea buckthorn (Hippophae rhamnoides), apricot (Prunus armeniaca) and roseroot (Rhodiola imbricata) from trans-Himalaya.MethodsThe 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and nitric oxide (NO) radical scavenging capacities and lipid peroxidation inhibition (LPI) property of the extracts were determined. Total antioxidant power was determined by ferric reducing/antioxidant power (FRAP) assay. Total polyphenol, flavonoid, flavonol, proanthocyanidin and carotenoid were also estimated for both extracts. We have identified and quantified the phyto-chemotypes present in the methanol and n-hexane extracts by hyphenated gas chromatography/mass spectrometry (GC/MS) technique.ResultsAntioxidant capacity assays using DPPH, ABTS, NO, LPI and FRAP exhibited analogous results where the phytococktail showed high antioxidant action. The phytococktail was also found to possess high quantity of total polyphenol, flavonoid, flavonol and carotenoid. A significant and linear correlation was found between the antioxidant capacities and bioactive principles. A total of 32 phyto-chemotypes were identified from these extracts by GC/MS chemometric fingerprinting. Major phyto-chemotypes identified by GC/MS were glycosides, phenylpropanoids and derivatives, terpenoids, alkaloids, phytosterols, fatty acids and esters, alkaloids and derivatives, organic acid esters and aromatic ethers with positive biological and pharmacological actions.ConclusionThe phytococktail extracts were found to contain considerable amount of diverse bioactive compounds with high antioxidant capacities. The presence of hydrophilic and lipophilic antioxidants in the phytococktail could have contributed to the higher antioxidant values. Hence, the phytococktail could be used as natural source of antioxidants to ameliorate disorders associated with oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Chemical composition and antioxidant capacities of phytococktail extracts from trans-Himalayan cold desert

Dhar

Bajpai

Tayade

et al. 2013

BMC Complement Altern Med

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“…We aimed to formulate the phytococktail comprising of sea buckthorn ( Hippophae rhamnoides L., Elaeagnaceae), apricot ( Prunus armeniaca L., Rosaceae) and roseroot ( Rhodiola imbricata Edgew., Crassulaceae) which could be capable of providing additional physiological benefits and meeting basic nutritional requirements in this extreme environment. The medicinal and therapeutic potential of the three plants have been extensively studied by previous researchers and these were reported to possess diverse bioactive constituents that ameliorate chronic diseases [34]–[38]. In our recent study, we have reported the antioxidant capacities and chemical composition of this phytococktail [39], [40].…”

Section: Introductionmentioning

confidence: 88%

“…Determination of vitamin content in the phytococktail was achieved according to the recent report from our institute [38].…”

Section: Methodsmentioning

confidence: 99%

Nutritional Profile of Phytococktail from Trans-Himalayan Plants

et al. 2013

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We estimated the nutritive value, vitamin content, amino acid composition, fatty acid content, and mineral profile of a phytococktail comprising sea buckthorn (Hippophae rhamnoides), apricot (Prunus armeniaca), and roseroot (Rhodiola imbricata) from trans-Himalaya. The free vitamin forms in the phytococktail were determined by rapid resolution liquid chromatography/tandem mass spectrometry (RRLC-MS/MS). Vitamin E and B-complex vitamins were detected as the principle vitamins. Reversed-phase high performance liquid chromatography (RP-HPLC) with pre-column derivatization was used for identification and quantification of amino acids. Eight essential and eleven non-essential amino acids were quantified, and the content ranged between 76.33 and 9485.67 µg/g. Among the essential amino acids, L-methionine, L-phenylalanine, L-lysine, L-leucine, and L-histidine were found to be the dominant contributors. We also quantified the fatty acids in the phytococktail by using gas chromatography coupled with a flame ionization detector (GC-FID) with fatty acid methyl esters (FAMEs) derivatization. The analysis revealed the presence of 4 major fatty acids contributing to the total lipid content. Palmitic acid was found to be the rich source of saturated fatty acid (SFA) and constituted ∼31% of the total lipid content. Among the unsaturated fatty acids (UFAs), palmitoleic acid (43.47%), oleic acid (20.89%), and linoleic acid (4.31%) were prominent. The mineral profiling was carried out by inductively coupled plasma optical emission spectrometer (ICP-OES), and it was found to contain a number of important dietary mineral elements. The harsh climatic conditions, difficult terrain, and logistic constraints at high altitude regions of Indian trans-Himalayan cold desert lead to the scarcity of fresh fruits and vegetables. Therefore, the source of multiple vitamins, essential amino acids, fatty acids, and dietary minerals from the phytococktail would provide great health benefit in the stressful environment and could be used as a high value nutritional supplement.

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“…Water-soluble vitamins were extracted by a combination of acid and enzymatic hydrolysis according to the method of Tayade et al (2013) with some modifications. Gooseberry pulp (10 g) was suspended in 10 mL of 0.1 N HCl and then treated at 100 °C for 20 min.…”

Section: Determinations Of Vitamins Bmentioning

confidence: 99%

Assessment of quality parameters and microbial characteristics of Cape gooseberry pulp (Physalis peruviana L.) subjected to high hydrostatic pressure treatment

Vega‐Gálvez

Díaz

López

et al. 2016

Food and Bioproducts Processing

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Sequential determination of fat- and water-soluble vitamins in Rhodiola imbricata root from trans-Himalaya with rapid resolution liquid chromatography/tandem mass spectrometry

Cited by 27 publications

References 51 publications

Chemical composition and antioxidant capacities of phytococktail extracts from trans-Himalayan cold desert

Chemical composition and antioxidant capacities of phytococktail extracts from trans-Himalayan cold desert

Nutritional Profile of Phytococktail from Trans-Himalayan Plants

Assessment of quality parameters and microbial characteristics of Cape gooseberry pulp (Physalis peruviana L.) subjected to high hydrostatic pressure treatment

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