Sequential Changes in Lipid Fluidity and Phase Properties of Microsomal Membranes from Senescing Rose Petals

Legge, Raymond L.; Thompson, John E.; Murr, Dennis P.; Tsujita, M. J.

doi:10.1093/jxb/33.2.303

Cited by 30 publications

(22 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Legge et al (13) also observed this increase in gel lipid with flower age. An increase in gel phase in the heterogenous membrane lipids at 22°C can also be inferred from the increase in the phase transition temperature of the isolated membrane lipids with flower age (14,16,22).…”

Section: Discussionmentioning

confidence: 68%

“…Such formation of gel phase lipid in cell membranes is characteristic of senescence in several leaves, cotyledons, and fruits (22). It also occurs in petal microsomal membranes ofanother rose cultivar (13). In that work with intact membranes it was possible that gel phase lipid was influenced by other membrane components such as protein.…”

Section: Discussionmentioning

confidence: 99%

“…This could be the cause of the observed increase in membrane permeability in rose petals ifthe observed phase separation occurred primarily in either the plasma membrane or tonoplast. At the same time as gel phase forms in the microsomal membranes there is a parallel change in the related, but different, physical property of the membrane lipids: lipid microviscosity increases (11) and conversely lipid fluidity decreases (13). Increased microviscosity was also suggested to lead to membrane malfunction and death (7).…”

Section: Discussionmentioning

confidence: 99%

“…Two different, but related, changes in physical properties of microsomal membranes have been reported in senescing rose petals: an increase in microviscosity and a corresponding decrease in fluidity (5,13); and an increase in lipid phase transition (gel to liquid-crystalline) temperature (13). However Flowers and Treatments.…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Changes in the Physical State of Membrane Lipids during Senescence of Rose Petals

Faragher

Wachtel²,

Mayak³

1987

Plant Physiol.

View full text Add to dashboard Cite

Changes in the physical state of microsomal membrane lipids during senescence of rose flower petals (Rosa hyb. L. cv Mercedes) were measured by x-ray diffraction analysis. During senescence of cut flowers held at 22°C, lipid in the ordered, gel phase appeared in the otherwise disordered, liquid-crystalline phase lipids of the membranes. This was due to an increase in the phase transition temperature of the lipids. The proportion of gel phase in the membrane lipids of 2-day-old flowers was estimated as about 20% at 22°C. Ethylene may be responsible, at least in part, for the increase in lipid transition temperature during senescence since aminooxyacetic acid and silver thiosulfate inhibited the rise in transition temperature. When flowers were stored at 3°C for 10 to 17 days and then transferrd to 22°C, gel phase lipid appeared in membranes earlier than in freshly cut flowers. This advanced senescence was the result of aging at 3°C, indicated by increases in membrane lipid transition temperature and ethylene production rate during the time at 3°C. It is concluded that changes in the physical state of membrane lipids are an integral part of senescence of rose petals, that they are caused, at least in part, by ethylene action and that they are responsible, at least in part, for the increase in membrane permeability which precedes flower death.cence processes have not been investigated. For example, it is not known whether the membrane changes precede, or are a result of, the increase in ethylene production. It is known that ethylene increases membrane permeability in rose petals (10) and that changes in the physical state of membrane lipids can increase membrane permeability (2,8). Therefore, it is reasonable to propose that ethylene may alter the physical properties of rose petal membranes and hence increase their permeability.In the work reported here we investigated changes in the physical state of microsomal membrane lipids during senescence of rose petals. The physical state of lipids was measured by a direct method, x-ray diffraction analysis, which reveals the phase properties and ordering of the lipid acyl chains ( 14). The changes in the membrane lipids have been related to other senescence processes (ethylene production and membrane permeability) during senescence, and after treatments which hastened or slowed senescence. In particular, the possible role of ethylene in regulating changes in membrane lipids was investigated. MATERIALS AND METHODSSenescence of rose petals involves a climacteric-like rise in ethylene production followed by increased membrane permeability to electrolytes, petal wilting, and death (10). Ethylene action is, at least in part, responsible for the increased membrane permeability which presumably leads to death (10,20). During plant senescence critical changes occur in the physical state of membrane lipids which alter the functional integrity of membranes and lead to cell death (7,16,22 Flowers and Treatments. Flowers of the rose (Rosa hyb L.) cv Mercedes were grown, cut, a...

show abstract

Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Changes in the Physical State of Membrane Lipids during Senescence of Rose Petals

Faragher

Wachtel²,

Mayak³

1987

Plant Physiol.

View full text Add to dashboard Cite

show abstract

“…Fluorescence depolarization measurements first revealed the gradual decrease in fluidity that occurs during senescence in rose and carnation petals (Borochov et al, 1976;Thompson, 1984;Itzhaki et al, 1990). Similar results were obtained using electron spin resonance (Legge et al, 1982). The increase in the sterol-to-phospholipid ratio observed in membranes of senescing tissues is thought to be responsible for this decrease in lipid fluidity (Borochov et al, 1978;Borochov and Woodson, 1989;Itzhaki et al, 1990).…”

mentioning

confidence: 79%

Deuterium Magnetic Resonance Studies of Senescence-Related Changes in the Physical Properties of Rose Petal Membrane Lipids

et al. 1995

View full text Add to dashboard Cite

The physical properties of membrane lipids in senescing rose (Rosa hybrida L., cv Mercedes) petals were studied by deuterium nuclear magnetic resonance ('H-NMR) and fluorescence depolarization. AI1 of the 'H-NMR spectra arising from deuterated dimyristoylphosphatidylcholine mixed with whole-lipid extracts from membranes of petals of different ages had a shape that is characteristic of liquid-crystalline lipid at 30°C. Arrhenius plots of the moments of the 'H spectra and fluorescence depolarization values measured from 1,6-diphenyl hexatriene-labeled rose petal membrane lipid samples indicated that membrane lipid order increased with decreasing temperature as well as with increasing age of the petals. The latter trend is explained by previously observed increases in fatty acid saturation and increases in the sterol-to-phospholipid ratio that occur in rose petals during senescence. The 'H-NMR spectra obtained at 0°C also contained quadrupolar splitting lines from lipid in the gel phase, confirming the occurrence of this phase in membranes from this tissue.

show abstract

The Role of Cell Wall Hydrolases in Fruit Softening

Huber¹

1983

Horticultural Reviews

View full text Add to dashboard Cite

Sequential Changes in Lipid Fluidity and Phase Properties of Microsomal Membranes from Senescing Rose Petals

Cited by 30 publications

References 18 publications

Changes in the Physical State of Membrane Lipids during Senescence of Rose Petals

Changes in the Physical State of Membrane Lipids during Senescence of Rose Petals

Deuterium Magnetic Resonance Studies of Senescence-Related Changes in the Physical Properties of Rose Petal Membrane Lipids

The Role of Cell Wall Hydrolases in Fruit Softening

Contact Info

Product

Resources

About