Changes in the Physical State of Membrane Lipids during Senescence of Rose Petals

Faragher, John D.; Wachtel, Ellen; Mayak, Shimon

doi:10.1104/pp.83.4.1037

Cited by 34 publications

(24 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This would be in line with findings for Ipomoea (2), Tradescantia (24), and rose (13) in which a decrease in the amount of phospholipid and its rate of synthesis can precede by several hours the ethylene climacteric and loss of turgor.…”

Section: Discussionsupporting

confidence: 75%

“…First, there is a decrease in the amount of phospholipid in Ipomoea (2), Tradescantia (24), and rose (7), due to both reduced synthesis and enhanced degradation. Second, there is an increase in the sterol to phospholipid ratio, leading to decreased membrane fluidity in Ipomoea (2), carnation (1,26), and rose (13) and to changes in the fatty acid component of the lipids (21). In our study, we looked at relative 32p incorporation into individual phospholipids rather than at their amounts.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Physiological Changes Accompanying Senescence in the Ephemeral Daylily Flower

Bieleski¹,

Reid²

1992

Plant Physiol.

View full text Add to dashboard Cite

The daylily flower, Hemerocallis hybrid cv Cradle Song, develops from the opening bud to full senescence in 36 hours. Unlike other ephemeral flowers studied to date, it does not respond to ethylene, but other senescence phenomena are similar. There was a small respiration climacteric coinciding with early flower senescence, and it was also observed in isolated petals and petal slices. Cycloheximide abolished the climacteric and delayed senescence in all three systems. Petal apparent free space increased from 30% at bud opening to 38% at the onset of senescence, and sugar efflux increased from 0.2 to 2.8 milligrams per gram of fresh weight per hour during the same period. A sharp increase in ion efflux from 0.8 to 4.0 micromoles of NaCI equivalents per gram of fresh weight per hour, coinciding with the climacteric, was abolished by cycloheximide. Uptake of radiolabeled inorganic phosphate by petal slices from 100 micromolar solution increased during onset of senescence from 6 to 10 nmoles per gram of fresh weight per hour. Half was esterified; of this, 14% went into ATP, and the cellular energy charge remained high at 0.86 during senescence. The proportion incorporated into phospholipid (2.2%) did not change during senescence, but the proportion in phosphatidyl choline increased and in phosphatidyl glycerol decreased during senescence. The general phosphate ester pattem in presenescent slices closely resembled that in other plant tissues except that phospholipid precursors were more prominent (approximately 20% of total organic 32p versus 5%). In senescent slices, the proportion of hexose phosphates decreased from 40 to 15% of total organic 32P and that of phospholipid precursors increased to approximately 50%, suggesting that phospholipid synthesis was blocked early in senescence.concentrated on five species: carnation, rose, morning glory (Ipomoea), hibiscus, and Tradescantia, the first four being dicotyledons and the last three ephemerals. Ephemeral flower senescence in particular has been an attractive and productive model system because events happen so quickly. Some features characterizing the process are ethylene production, increased respiration, induction ofcatabolic enzymes, a decrease in protein, polysaccharide and nucleic acid content, and a loss of membrane function. Perhaps the most central question to emerge has concerned the extent to which ethylene triggers petal senescence and is the driving force behind it or is simply one of its manifestations (8).Hemerocallis, the daylily, is also an ephemeral flower, in the Liliaceae, and one of an important group of cut flowers that has received little research attention. The feature of particular interest to us is that ethylene appears not to be involved in its senescence (16; M. Lay-Yee, A.D. Stead, and M.S. Reid, manuscript in preparation). Therefore, it may prove useful for studying non-ethylene-mediated senescence, particularly in relation to the nature of various membranerelated phenomena. The hypothesis addressed here is that the rapid wiltin...

show abstract

Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Physiological Changes Accompanying Senescence in the Ephemeral Daylily Flower

Bieleski¹,

Reid²

1992

Plant Physiol.

View full text Add to dashboard Cite

show abstract

“…Indeed, the accumulation of these metabolites in membranes and ensuing lipid-phase separations are well-established manifestations of senescence for a number of plant tissues (Barber and Thompson, 1983;Platt-Aloia and Thomson, 1985;Faragher et al, 1987) and have been shown to correlate temporally with impairment of blebbing (Yao et al, 1991b;Hudak et al, 1995). Particles of similar size and shape to those isolated from the cytosol are evident in the cytoplasm of intact cells in electron micrographs of thin-sectioned carnation petal tissue (Hudak and Thompson, 1996), which supports the contention that they are in situ elements of the cytosol.…”

Section: Discussionmentioning

confidence: 99%

Subcellular Localization of Secondary Lipid Metabolites Including Fragrance Volatiles in Carnation Petals

Hudak

Thompson

1997

Plant Physiology

View full text Add to dashboard Cite

Pulse-chase labeling of carnation (Dianfhus caryophyllus L. cv lmproved White Sim) petals with [14C]acetate has provided evidente for a hydrophobic subcompartment of lipid-protein particles within the cytosol that resemble oil bodies, are formed by blebbing from membranes, and are enriched i n lipid metabolites (including fragrance volatiles) derived from membrane fatty acids. Fractionation of the petals during pulse-chase labeling revealed that radiolabeled fatty acids appear first in microsomal membranes and subsequently i n cytosolic lipid-protein particles, indicating that the particles originate from membranes. This interpretation is supported by the finding that the cytosolic lipid-protein particles contain phospholipid as well as the same fatty acids found i n microsomal membranes. Radiolabeled polar lipid metabolites (methanol/ water-soluble) were detectable in both in situ lipid-protein particles isolated from the cytosol and those generated in vitro from isolated radiolabeled microsomal membranes. The lipid-protein particles were also enriched in hexanal, frans-2-hexenal, 1 -hexanol, 3-hexen-1-01, and 2-hexanol, volatiles of carnation flower fragrance that are derived from membrane fatty acids through the lipoxygenase pathway. Therefore, secondary lipid metabolites, including components of fragrance, appear t o be formed within membranes of peta1 tissue and are subsequently released from the membrane bilayers into the cytosol by blebbing of lipid-protein particles.

show abstract

“…In all plant tissues studied so far, aging cell membranes exhibit gradual changes in the physical properties of their component lipids, including a decrease in their fluidity (3) and an increasing proportion of lipid domains in the gel phase (6,14). These changes precede the loss in the capacity of the membranes to act as a hydrophobic barrier (6).…”

Section: Plant Materialsmentioning

confidence: 99%

Age-Related Changes in Petal Membranes from Attached and Detached Rose Flowers

Itzhaki¹,

Borochov²,

Mayak³

1990

Plant Physiol.

Self Cite

View full text Add to dashboard Cite

Changes in petal membrane properties during aging were studied in cut and in attached rose flowers (Rosa hybrida L., cv Mercedes). Both cut and attached flowers exhibited a growth phase characterized by an increase in fresh weight and an accumulation of membrane components. The growth phase, which was more pronounced in the attached than in the cut flowers, was followed by a senescence phase, characterized by a decrease in fresh weight and a decline in membrane components. In cut flowers, both the growth and the senescence phases were accompanied by a decrease in membrane fluidity and in the ratio of unsaturated to saturated fatty acids, but the ratio of sterol to phospholipid increased. In attached flowers, while both the membrane fluidity and the sterol-to-phospholipid ratio remained unchanged during the growth phase, the senescence phase was accompanied (as in cut flowers) by a decrease in membrane fluidity and an increase in the sterol-to-phospholipid ratio. Unlike in cut flowers, however, the age-related changes in the ratio of unsaturation of fatty acids were not correlated with those of fluidity. Changes in the saturation of phospholipid acyl chains are commonly thought to influence membrane fluidity. Our observations question this view and suggest instead that the ratio of sterol to phospholipid may play the major role in maintaining membrane lipid fluidity.The above changes have been found to correlate well with the age-related decrease in membrane lipid fluidity (3).In the present work, the aging processes in petal membranes were studied using both cut and attached rose flowers, which are known to age at different rates (8). In particular, the composition of the membranes during the life-span of the flower was analyzed in detail. MATERIALS AND METHODS Plant

show abstract

Changes in the Physical State of Membrane Lipids during Senescence of Rose Petals

Cited by 34 publications

References 16 publications

Physiological Changes Accompanying Senescence in the Ephemeral Daylily Flower

Physiological Changes Accompanying Senescence in the Ephemeral Daylily Flower

Subcellular Localization of Secondary Lipid Metabolites Including Fragrance Volatiles in Carnation Petals

Age-Related Changes in Petal Membranes from Attached and Detached Rose Flowers

Contact Info

Product

Resources

About